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You searched for: EV100021 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV100021 | 1/2 | Homo sapiens | Cell culture supernatant | (d)(U)C Filtration |
Lenassi M | 2010 | 33% | |
Study summaryFull title
All authors
Lenassi M, Cagney G, Liao M, Vaupotic T, Bartholomeeusen K, Cheng Y, Krogan NJ, Plemenitas A, Peterlin BM
Journal
Traffic
Abstract
The HIV accessory protein negative factor (Nef) is one of the earliest and most abundantly expressed (show more...)
EV-METRIC
33% (61st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C + Filtration
Protein markers
EV: TSG101/ CD63/ HSC70/ CD81/ Alix/ Annexin2/ LAMP2/ ICAM1/ AChE/ Beta-actin/ CD9/ MHC1
non-EV: Cell organelle protein Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
60
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ CD81/ CD9/ TSG101/ HSC70/ LAMP2/ AChE/ Beta-actin/ Annexin2/ ICAM1/ MHC1
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
HSC70/ LAMP2/ AChE/ Beta-actin/ Annexin2/ ICAM1/ MHC1
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
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EV100021 | 2/2 | Homo sapiens | Cell culture supernatant | DG (d)(U)C Filtration |
Lenassi M | 2010 | 25% | |
Study summaryFull title
All authors
Lenassi M, Cagney G, Liao M, Vaupotic T, Bartholomeeusen K, Cheng Y, Krogan NJ, Plemenitas A, Peterlin BM
Journal
Traffic
Abstract
The HIV accessory protein negative factor (Nef) is one of the earliest and most abundantly expressed (show more...)
EV-METRIC
25% (51st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C + Filtration
Protein markers
EV: AChE
non-EV: Proteomics
no
EV density (g/ml)
1.037-1.055
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Density gradient
Density medium
Iodixanol
Lowest density fraction
6
Highest density fraction
18
Orientation
Top-down
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
AChE
ELISA
Detected EV-associated proteins
AChE
Characterization: Particle analysis
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