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You searched for: 2012 (Year of publication)
Showing 151 - 200 of 268
Showing 151 - 200 of 268
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120119 | 1/2 | Homo sapiens | Serum | (d)(U)C | Ciravolo V | 2012 | 14% | |
Study summaryFull title
All authors
Ciravolo V, Huber V, Ghedini GC, Venturelli E, Bianchi F, Campiglio M, Morelli D, Villa A, Della Mina P, Menard S, Filipazzi P, Rivoltini L, Tagliabue E, Pupa SM
Journal
J Cell Physiol
Abstract
Exosomes are endosome-derived nanovesicles actively released into the extracellular environment and (show more...)
EV-METRIC
14% (55th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
253.9 (pelleting) / 60.38 (washing)
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW28
Pelleting: adjusted k-factor
253.9
Wash: Rotor Type
TLA100.3
Wash: adjusted k-factor
60.38
|
||||||||
EV120054 | 1/1 | Homo sapiens | NAY |
(d)(U)C DG |
Cho JA | 2012 | 14% | |
Study summaryFull title
All authors
Cho JA, Park H, Lim EH, Lee KW
Journal
Int J Oncol
Abstract
Exosomes are small membrane vesicles secreted into the extracellular environment by various types of (show more...)
EV-METRIC
14% (44th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2.5
Orientation
Bottom-up
Speed (g)
100000
|
||||||||
EV120115 | 1/1 | Vibrio cholerae | Bacteria |
(d)(U)C Filtration |
Bishop AL | 2012 | 14% | |
Study summaryFull title
All authors
Bishop AL, Tarique AA, Patimalla B, Calderwood SB, Qadri F, Camilli A
Journal
J Infect Dis
Abstract
BACKGROUND: Vibrio cholerae excreted by cholera patients is hyperinfectious (HI), which can be model (show more...)
EV-METRIC
14% (56th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bacteria
Sample origin
NAY
Focus vesicles
OMV
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
182.8 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Vibrio cholerae
Sample Type
Bacteria
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
240
Pelleting: rotor type
SW32;50.2Ti
Pelleting: adjusted k-factor
182.8
Filtration steps
0.45µm > x > 0.22µm, 0.22µm or 0.2µm
|
||||||||
EV210239 | 1/3 | Homo sapiens | Blood plasma | (d)(U)C | Marques FK | 2012 | 13% | |
Study summaryFull title
All authors
Marques FK, Campos FM, Filho OA, Carvalho AT, Dusse LM, Gomes KB
Journal
Clin Chim Acta
Abstract
The present study aimed to evaluate microparticles (MPs) from different sources in women with severe (show more...)
EV-METRIC
13% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Healthy non-pregnant
Focus vesicles
microparticle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD66/ CD51/ CD14/ CD41/ CD45/ CD235a/ CD3
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
Not specified
Pelleting: speed (g)
14000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry aspecific beads
Antibody details provided?
Yes
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Detected EV-associated proteins
CD66/ CD51/ CD14/ CD41/ CD45/ CD235a/ CD3
Characterization: Lipid analysis
No
|
||||||||
EV210239 | 2/3 | Homo sapiens | Blood plasma | (d)(U)C | Marques FK | 2012 | 13% | |
Study summaryFull title
All authors
Marques FK, Campos FM, Filho OA, Carvalho AT, Dusse LM, Gomes KB
Journal
Clin Chim Acta
Abstract
The present study aimed to evaluate microparticles (MPs) from different sources in women with severe (show more...)
EV-METRIC
13% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Healthy pregnant
Focus vesicles
microparticle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD66/ CD51/ CD14/ CD41/ CD45/ CD235a/ CD3
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
Not specified
Pelleting: speed (g)
14000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry aspecific beads
Antibody details provided?
Yes
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Detected EV-associated proteins
CD66/ CD51/ CD14/ CD41/ CD45/ CD235a/ CD3
Characterization: Lipid analysis
No
|
||||||||
EV210239 | 3/3 | Homo sapiens | Blood plasma | (d)(U)C | Marques FK | 2012 | 13% | |
Study summaryFull title
All authors
Marques FK, Campos FM, Filho OA, Carvalho AT, Dusse LM, Gomes KB
Journal
Clin Chim Acta
Abstract
The present study aimed to evaluate microparticles (MPs) from different sources in women with severe (show more...)
EV-METRIC
13% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Pregnant, severe preeclampsia
Focus vesicles
microparticle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD66/ CD51/ CD14/ CD41/ CD45/ CD235a/ CD3
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
Not specified
Pelleting: speed (g)
14000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry aspecific beads
Antibody details provided?
Yes
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Detected EV-associated proteins
CD66/ CD51/ CD14/ CD41/ CD45/ CD235a/ CD3
Characterization: Lipid analysis
No
|
||||||||
EV120102 | 2/2 | Mus musculus | Blood plasma | Microfluidics | Davies RT | 2012 | 13% | |
Study summaryFull title
All authors
Davies RT, Kim J, Jang SC, Choi EJ, Gho YS, Park J
Journal
Lab Chip
Abstract
Extracellular vesicles are released by various cell types, particularly tumor cells, and may be pote (show more...)
EV-METRIC
13% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
Microfluidics
Protein markers
EV: CD9
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Mus musculus
Sample Type
Blood plasma
Separation Method
Other
Name other separation method
Microfluidics
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV120167 | 1/2 | Mus musculus | NAY | ExoQuick | Wang G | 2012 | 13% | |
Study summaryFull title
All authors
Wang G, Dinkins M, He Q, Zhu G, Poirier C, Campbell A, Mayer-Proschel M, Bieberich E
Journal
J Biol Chem
Abstract
Amyloid protein is well known to induce neuronal cell death, whereas only little is known about its (show more...)
EV-METRIC
13% (34th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: Alix/ PKC/ TSG101/ PAR-4
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ TSG101/ PAR-4/ PKC
ELISA
Antibody details provided?
No
Detected EV-associated proteins
PAR-4/ PKC
Characterization: Particle analysis
None
|
||||||||
EV120087 | 3/3 | Mus musculus | Serum | ExoQuick | Singh PP | 2012 | 13% | |
Study summaryFull title
All authors
Singh PP, Smith VL, Karakousis PC, Schorey JS
Journal
J Immunol
Abstract
More than 2 billion people are infected with Mycobacterium. tuberculosis; however, only 5-10% of tho (show more...)
EV-METRIC
13% (47th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Serum
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD9
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120153 | 1/1 | Mus musculus | NAY |
DC Filtration UF |
Qi H | 2012 | 13% | |
Study summaryFull title
All authors
Qi H, Liu JP, Deng CY, Zhou HX, Deng SP, Li FR
Journal
Immunol Res
Abstract
Selective interference with CD45RB isoform by monoclonal antibody (anti-CD45RBmAb) reliably induces (show more...)
EV-METRIC
13% (34th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
DC
Filtration UF Protein markers
EV: CD80/ CD83/ MHC2/ CD86
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
Separation Method
Filtration steps
> 0.45 µm,
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD80/ CD83/ CD86/ MHC2
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD80/ CD83/ CD86/ MHC2
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120072 | 1/1 | Mus musculus | NAY |
(d)(U)C DC UF |
Li X | 2012 | 13% | |
Study summaryFull title
All authors
Li X, Li JJ, Yang JY, Wang DS, Zhao W, Song WJ, Li WM, Wang JF, Han W, Zhang ZC, Yu Y, Cao DY, Dou KF
Journal
PLoS One
Abstract
BACKGROUND: Dendritic cells (DCs) release bioactive exosomes that play an important role in immune r (show more...)
EV-METRIC
13% (34th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DC UF Protein markers
EV: MFGE8/ CD80/ HSP90/ CD86/ ICAM1/ HSP70/ Beta-actin/ MHC2/ MHC1
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Pelleting performed
No
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
HSP90/ HSP70/ Beta-actin/ MFGE8/ MHC1/ MHC2/ CD80/ CD86/ ICAM1
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Beta-actin/ MFGE8/ MHC1/ MHC2/ CD80/ CD86/ ICAM1
Characterization: Particle analysis
EM
EM-type
transmission EM
|
||||||||
EV120031 | 1/2 | Homo sapiens | Serum | ExoQuick | Epple LM | 2012 | 13% | |
Study summaryFull title
All authors
Epple LM, Griffiths SG, Dechkovskaia AM, Dusto NL, White J, Ouellette RJ, Anchordoquy TJ, Bemis LT, Graner MW
Journal
PLoS One
Abstract
Medulloblastomas are the most prevalent malignant pediatric brain tumors. Survival for these patient (show more...)
EV-METRIC
13% (47th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes / extracellular vesicles
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: HSP70/ CD63/ CD9/ HSC70
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD9/ HSP70/ HSC70
ELISA
Antibody details provided?
No
Detected EV-associated proteins
HSC70
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV120056 | 1/1 | Mus musculus | NAY | Deng Z | 2012 | 13% | ||
Study summaryFull title
All authors
Deng Z, Cheng Z, Xiang X, Yan J, Zhuang X, Liu C, Jiang H, Ju S, Zhang L, Grizzle W, Mobley J, Roman J, Miller D, Zhang HG
Journal
Am J Pathol
Abstract
Exosomes participate in intercellular communication, but most data published are based on exosomes r (show more...)
EV-METRIC
13% (34th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
Protein markers
EV: TSG101/ CD63/ CD9
non-EV: Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
Separation Method
Other
Name other separation method
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD9/ TSG101
Characterization: Particle analysis
None
|
||||||||
EV120102 | 1/2 | Mus musculus | Blood plasma | (d)(U)C | Davies RT | 2012 | 11% | |
Study summaryFull title
All authors
Davies RT, Kim J, Jang SC, Choi EJ, Gho YS, Park J
Journal
Lab Chip
Abstract
Extracellular vesicles are released by various cell types, particularly tumor cells, and may be pote (show more...)
EV-METRIC
11% (25th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD9
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Mus musculus
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
120
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120100 | 1/1 | Mus musculus | BALF | (d)(U)C | Zhu M | 2012 | 11% | |
Study summaryFull title
All authors
Zhu M, Li Y, Shi J, Feng W, Nie G, Zhao Y
Journal
Small
Abstract
Evaluation of systemic biosafety of nanomaterials urgently demands a comprehensive understanding of (show more...)
EV-METRIC
11% (35th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
BALF
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ CD80/ MHC2/ MHC1
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
BALF
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TSG101/ MHC1/ MHC2/ CD80
ELISA
Antibody details provided?
No
Detected EV-associated proteins
MHC1/ MHC2/ CD80
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120099 | 1/2 | Mus musculus | NAY | (d)(U)C | Yuyama K | 2012 | 11% | |
Study summaryFull title
All authors
Yuyama K, Sun H, Mitsutake S, Igarashi Y
Journal
J Biol Chem
Abstract
Amyloid ?-peptide (A?), the pathogenic agent of Alzheimer disease, is a physiological metabolite who (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Alix/ TSG101/ GM1
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ TSG101/ GM1
ELISA
Antibody details provided?
No
Detected EV-associated proteins
GM1
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120098 | 1/2 | Mus musculus | Blood plasma |
(d)(U)C IAF |
Yang C | 2012 | 11% | |
Study summaryFull title
All authors
Yang C, Ruffner MA, Kim SH, Robbins PD
Journal
Eur J Immunol
Abstract
Tumor-specific immunosuppression is frequently observed in tumor-bearing hosts. Exosomes are nano-si (show more...)
EV-METRIC
11% (25th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
IAF Protein markers
EV: MHC2/ CD9/ MFGE8
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Immunoaffinity capture
Selected surface protein(s)
MHC2
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ MFGE8/ MHC2
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD9/ MFGE8/ MHC2
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV120098 | 2/2 | Mus musculus | NAY |
(d)(U)C Filtration UF |
Yang C | 2012 | 11% | |
Study summaryFull title
All authors
Yang C, Ruffner MA, Kim SH, Robbins PD
Journal
Eur J Immunol
Abstract
Tumor-specific immunosuppression is frequently observed in tumor-bearing hosts. Exosomes are nano-si (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Protein markers
EV: Alix/ MHC2/ MFGE8
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ MFGE8/ MHC2
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Alix/ MFGE8/ MHC2
Characterization: Particle analysis
None
|
||||||||
EV120096 | 1/2 | Homo sapiens | Serum | (d)(U)C | Welker MW | 2012 | 11% | |
Study summaryFull title
All authors
Welker MW, Reichert D, Susser S, Sarrazin C, Martinez Y, Herrmann E, Zeuzem S, Piiper A, Kronenberger B
Journal
PLoS One
Abstract
AIM: Cellular CD81 is a well characterized hepatitis C virus (HCV) entry factor, while the relevance (show more...)
EV-METRIC
11% (40th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD81
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81
Characterization: Particle analysis
None
|
||||||||
EV120096 | 2/2 | Homo sapiens | Blood plasma | (d)(U)C | Welker MW | 2012 | 11% | |
Study summaryFull title
All authors
Welker MW, Reichert D, Susser S, Sarrazin C, Martinez Y, Herrmann E, Zeuzem S, Piiper A, Kronenberger B
Journal
PLoS One
Abstract
AIM: Cellular CD81 is a well characterized hepatitis C virus (HCV) entry factor, while the relevance (show more...)
EV-METRIC
11% (25th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD81
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81
Characterization: Particle analysis
None
|
||||||||
EV120167 | 2/2 | Mus musculus | NAY |
(d)(U)C Filtration IAF |
Wang G | 2012 | 11% | |
Study summaryFull title
All authors
Wang G, Dinkins M, He Q, Zhu G, Poirier C, Campbell A, Mayer-Proschel M, Bieberich E
Journal
J Biol Chem
Abstract
Amyloid protein is well known to induce neuronal cell death, whereas only little is known about its (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration IAF Protein markers
EV: TSG101/ PAR-4
non-EV: Actin/ GAPDH Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Filtration steps
0.22µm or 0.2µm
Immunoaffinity capture
Selected surface protein(s)
ceramide, CD9
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TSG101/ PAR-4
Detected contaminants
GAPDH/ Actin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
PAR-4
Characterization: Particle analysis
None
|
||||||||
EV120094 | 2/2 | Homo sapiens | Blood plasma |
(d)(U)C Filtration |
Wahlgren J | 2012 | 11% | |
Study summaryFull title
All authors
Wahlgren J, De L Karlson T, Brisslert M, Vaziri Sani F, Telemo E, Sunnerhagen P, Valadi H
Journal
Nucleic Acids Res
Abstract
Despite the promise of RNA interference (RNAi) and its potential, e.g. for use in cancer therapy, se (show more...)
EV-METRIC
11% (25th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: CD81/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ CD9
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Selected surface protein(s)
Yes
Characterization: Particle analysis
DLS
|
||||||||
EV120163 | 1/1 | Homo sapiens | NAY |
(d)(U)C DC |
Truman JP | 2012 | 11% | |
Study summaryFull title
All authors
Truman JP, Al Gadban MM, Smith KJ, Jenkins RW, Mayroo N, Virella G, Lopes-Virella MF, Bielawska A, Hannun YA, Hammad SM
Journal
Immunology
Abstract
Oxidized low-density lipoprotein (oxLDL) and oxLDL-containing immune complexes (oxLDL-IC) contribute (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
DC Adj. k-factor
255.8 (pelleting)
Protein markers
EV: HSP70
non-EV: Proteomics
no
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
240
Pelleting: rotor type
SW41
Pelleting: adjusted k-factor
255.8
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
HSP70
Characterization: Particle analysis
None
|
||||||||
EV120091 | 1/1 | Homo sapiens | BALF |
(d)(U)C IAF |
Torregrosa Paredes P | 2012 | 11% | |
Study summaryFull title
All authors
Torregrosa Paredes P, Esser J, Admyre C, Nord M, Rahman QK, Lukic A, Rådmark O, Grönneberg R, Grunewald J, Eklund A, Scheynius A, Gabrielsson S
Journal
Allergy
Abstract
BACKGROUND: Leukotrienes (LTs) are potent pro-inflammatory mediators involved in asthma. Exosomes, n (show more...)
EV-METRIC
11% (35th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
BALF
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
IAF Protein markers
EV: CD63/ CD81/ CD86/ CD107a/ Beta-actin/ MHC2
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
BALF
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Immunoaffinity capture
Selected surface protein(s)
MHC2
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ CD86/ CD107a/ MHC2/ Beta-actin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD86/ CD107a/ MHC2/ Beta-actin
Characterization: Particle analysis
None
|
||||||||
EV120090 | 1/3 | Homo sapiens | NAY | (d)(U)C | Tolosa JM | 2012 | 11% | |
Study summaryFull title
All authors
Tolosa JM, Schjenken JE, Clifton VL, Vargas A, Barbeau B, Lowry P, Maiti K, Smith R
Journal
Placenta
Abstract
OBJECTIVES: To examine whether syncytin-1 has immune regulatory functions and is carried by human pl (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
No
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TSG101
Characterization: Particle analysis
None
|
||||||||
EV120160 | 1/2 | Homo sapiens | NAY |
(d)(U)C DC Filtration |
Taverna S | 2012 | 11% | |
Study summaryFull title
All authors
Taverna S, Flugy A, Saieva L, Kohn EC, Santoro A, Meraviglia S, De Leo G, Alessandro R
Journal
Int J Cancer
Abstract
Our study is designed to assess if exosomes released from chronic myelogenous leukemia (CML) cells m (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DC Filtration Protein markers
EV: AChE/ CD63/ HSC70
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ HSC70/ AChE
ELISA
Antibody details provided?
No
Detected EV-associated proteins
HSC70/ AChE
Characterization: Particle analysis
EM
EM-type
scanning EM
Image type
Wide-field
Report size (nm)
Not reported
|
||||||||
EV120107 | 1/2 | Homo sapiens | NAY | (d)(U)C | Saman S | 2012 | 11% | |
Study summaryFull title
All authors
Saman S, Kim W, Raya M, Visnick Y, Miro S, Saman S, Jackson B, McKee AC, Alvarez VE, Lee NC, Hall GF
Journal
J Biol Chem
Abstract
Recent demonstrations that the secretion, uptake, and interneuronal transfer of tau can be modulated (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: EEA1/ Alix/ Flotilin1
non-EV: Proteomics
yes
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ Flotilin1/ EEA1
ELISA
Antibody details provided?
No
Detected EV-associated proteins
EEA1
Characterization: Particle analysis
EM
EM-type
immune EM
EM protein
Alix
Image type
Wide-field
|
||||||||
EV120107 | 2/2 | Homo sapiens | "Cerebrospinal fluid" |
(d)(U)C DG |
Saman S | 2012 | 11% | |
Study summaryFull title
All authors
Saman S, Kim W, Raya M, Visnick Y, Miro S, Saman S, Jackson B, McKee AC, Alvarez VE, Lee NC, Hall GF
Journal
J Biol Chem
Abstract
Recent demonstrations that the secretion, uptake, and interneuronal transfer of tau can be modulated (show more...)
EV-METRIC
11% (31st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
"Cerebrospinal fluid"
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Alix
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
"Cerebrospinal fluid"
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Density gradient
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Bottom-up
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix
Characterization: Particle analysis
None
|
||||||||
EV120082 | 1/1 | Ovis aries | NAY |
(d)(U)C DG |
Racicot K | 2012 | 11% | |
Study summaryFull title
All authors
Racicot K, Schmitt A, Ott T
Journal
Am J Reprod Immunol
Abstract
PROBLEM: Dairy cattle suffer from high percentages of early embryonic loss, and therefore, it is cri (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Alix
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Ovis aries
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
10
Highest density fraction
60
Orientation
Bottom-up
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120154 | 1/1 | Homo sapiens | NAY | (d)(U)C | Qiu S | 2012 | 11% | |
Study summaryFull title
All authors
Qiu S, Duan X, Geng X, Xie J, Gao H
Journal
Asian Pac J Allergy Immunol
Abstract
BACKGROUND: The prevalence of chronic rhinitis is increasing rapidly. Its pathogenesis is not fully (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: SEB/ Derp1
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
SEB/ Derp1
ELISA
Antibody details provided?
No
Detected EV-associated proteins
SEB/ Derp1
Characterization: Particle analysis
EM
EM-type
immune EM
EM protein
Derp1; SEB
Image type
Wide-field
|
||||||||
EV120147 | 1/1 | Acinetobacter baumannii | Bacteria |
(d)(U)C Filtration UF |
Moon DC | 2012 | 11% | |
Study summaryFull title
Acinetobacter baumannii outer membrane protein A modulates the biogenesis of outer membrane vesicles
All authors
Moon DC, Choi CH, Lee JH, Choi CW, Kim HY, Park JS, Kim SI, Lee JC
Journal
J Microbiol
Abstract
Acinetobacter baumannii secretes outer membrane vesicles (OMVs) during both in vitro and in vivo gro (show more...)
EV-METRIC
11% (40th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bacteria
Sample origin
NAY
Focus vesicles
OMV
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Protein markers
EV: OmpA
non-EV: Proteomics
yes
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Acinetobacter baumannii
Sample Type
Bacteria
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
180
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
OmpA
ELISA
Antibody details provided?
No
Detected EV-associated proteins
OmpA
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120078 | 1/1 | Homo sapiens | NAY |
(d)(U)C Filtration |
Mineo M | 2012 | 11% | |
Study summaryFull title
All authors
Mineo M, Garfield SH, Taverna S, Flugy A, De Leo G, Alessandro R, Kohn EC
Journal
Angiogenesis
Abstract
Exosomes, microvesicles of endocytic origin released by normal and tumor cells, play an important ro (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: CD81/ TSG101/ CD63
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ TSG101
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV120075 | 1/1 | Homo sapiens | NAY | (d)(U)C | Makhadiyeva D | 2012 | 11% | |
Study summaryFull title
All authors
Makhadiyeva D, Lam L, Moatari M, Vallance J, Zheng Y, Campbell EC, Powis SJ
Journal
Immunology
Abstract
Many MHC class I molecules contain unpaired cysteine residues in their cytoplasmic tail domains, the (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: MHC1
non-EV: Proteomics
no
Show all info
Study aim
Other/MHC1 assembly
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
MHC1
ELISA
Antibody details provided?
No
Detected EV-associated proteins
MHC1
Characterization: Particle analysis
None
|
||||||||
EV120074 | 1/1 | Homo sapiens | NAY |
(d)(U)C Filtration UF |
Lim JW | 2012 | 11% | |
Study summaryFull title
All authors
Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ
Journal
Electrophoresis
Abstract
Mutations in the adenomatous polyposis coli (APC) tumor suppressor gene are common in both inherited (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Protein markers
EV: Alix/ HSP70/ TSG101/ CD9
non-EV: Proteomics
yes
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Filtration steps
0.2µm > x > 0.1µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD9/ HSP70/ TSG101
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120073 | 1/1 | Homo sapiens | Urine | (d)(U)C | Li ZZ | 2012 | 11% | |
Study summaryFull title
All authors
Li ZZ, Zhao ZZ, Wen JG, Xing L, Zhang H, Zhang Y
Journal
J Pediatr Surg
Abstract
BACKGROUND/PURPOSE: Down-regulation of aquaporin 1 (AQP1) and up-regulation of transforming growth f (show more...)
EV-METRIC
11% (33rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: AQP1
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
AQP1
ELISA
Antibody details provided?
No
Detected EV-associated proteins
AQP1
Characterization: Particle analysis
None
|
||||||||
EV120069 | 1/1 | Homo sapiens | NAY |
(d)(U)C Filtration |
Lau CS | 2012 | 11% | |
Study summaryFull title
All authors
Lau CS, Wong DT
Journal
PLoS One
Abstract
Saliva is a useful biofluid for the early detection of disease, but how distal tumors communicate wi (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes / microvesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: Beta-actin/ CD63
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ Beta-actin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Beta-actin
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120022 | 2/2 | Homo sapiens | Trophoblasts; PMBC | (d)(U)C | Kshirsagar SK | 2012 | 11% | |
Study summaryFull title
All authors
Kshirsagar SK, Alam SM, Jasti S, Hodes H, Nauser T, Gilliam M, Billstrand C, Hunt JS, Petroff MG
Journal
Placenta
Abstract
The semiallogenic fetus is tolerated by the maternal immune system through control of innate and ada (show more...)
EV-METRIC
11% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Trophoblasts; PMBC
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: B7H-1/ CD63/ LAMP1/ MHC1
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Trophoblasts; PMBC
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ LAMP1/ MHC1/ B7H-1
ELISA
Antibody details provided?
No
Detected EV-associated proteins
LAMP1/ MHC1/ B7H-1
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV120068 | 1/1 | Homo sapiens | NAY | (d)(U)C | Kloft N | 2012 | 11% | |
Study summaryFull title
All authors
Kloft N, Neukirch C, von Hoven G, Bobkiewicz W, Weis S, Boller K, Husmann M
Journal
J Biol Chem
Abstract
The constitutive reverter of eIF2? phosphorylation (CReP)/PPP1r15B targets the catalytic subunit of (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: AChE/ Beta-actin
non-EV: Proteomics
no
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Beta-actin/ AChE
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Beta-actin/ AChE
Characterization: Particle analysis
None
|
||||||||
EV120062 | 1/1 | Homo sapiens | NAY |
(d)(U)C filter |
Goda T | 2012 | 11% | |
Study summaryFull title
All authors
Goda T, Masuno K, Nishida J, Kosaka N, Ochiya T, Matsumoto A, Miyahara Y
Journal
Chem Commun
Abstract
We report a method for detecting microRNAs encapsulated in exosomes using a microelectrode array in (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
filter Protein markers
EV: CD63
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
No
Other
Name other separation method
filter
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63
Characterization: Particle analysis
DLS
NTA
|
||||||||
EV120059 | 1/1 | Homo sapiens | NAY | (d)(U)C | Fleming JM | 2012 | 11% | |
Study summaryFull title
All authors
Fleming JM, Ginsburg E, Oliver SD, Goldsmith P, Vonderhaar BK
Journal
BMC Cancer
Abstract
BACKGROUND: Recent evidence suggests an emerging role for S100 protein in breast cancer and tumor pr (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: GAPDH
non-EV: Proteomics
no
Show all info
Study aim
Other/Hornerin expression/distribution
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
80
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
GAPDH
ELISA
Antibody details provided?
No
Detected EV-associated proteins
GAPDH
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120058 | 1/1 | Homo sapiens | NAY | (d)(U)C | Fang YT | 2012 | 11% | |
Study summaryFull title
All authors
Fang YT, Lin CF, Wang CY, Anderson R, Lin YS
Journal
J Cell Physiol
Abstract
Annexin A2 (p36) is usually present together with its natural ligand p11 as a heterotetramer complex (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ Annexin2
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Other/Annexin2 surface translocation mechanism
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TSG101/ Annexin2
Detected contaminants
Cell organelle protein
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Annexin2
Characterization: Particle analysis
None
|
||||||||
EV120055 | 1/1 | Homo sapiens | Urine | (d)(U)C | Del Boccio P | 2012 | 11% | |
Study summaryFull title
All authors
Del Boccio P, Raimondo F, Pieragostino D, Morosi L, Cozzi G, Sacchetta P, Magni F, Pitto M, Urbani A
Journal
Electrophoresis
Abstract
Urinary exosomes are released from every renal epithelial cell type facing the urinary space and the (show more...)
EV-METRIC
11% (33rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Alix/ TSG101
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ TSG101
Characterization: Particle analysis
None
|
||||||||
EV120122 | 1/1 | Homo sapiens Mus musculus |
NAY |
(d)(U)C Filtration |
Danzer KM | 2012 | 11% | |
Study summaryFull title
All authors
Danzer KM, Kranich LR, Ruf WP, Cagsal-Getkin O, Winslow AR, Zhu L, Vanderburg CR, McLean PJ
Journal
Mol Neurodegener
Abstract
BACKGROUND: Aggregation of alpha-synuclein (?syn) and resulting cytotoxicity is a hallmark of sporad (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: Alix/ Flotillin
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens / Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Filtration steps
0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ Flotillin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Flotillin
Characterization: Particle analysis
EM
EM-type
transmission EM/ immune EM
EM protein
CD63
Image type
Wide-field
|
||||||||
EV120121 | 1/1 | Homo sapiens | NAY | (d)(U)C | Corrado C | 2012 | 11% | |
Study summaryFull title
All authors
Corrado C, Flugy AM, Taverna S, Raimondo S, Guggino G, Karmali R, De Leo G, Alessandro R
Journal
PLoS One
Abstract
The Bcr/Abl kinase has been targeted for the treatment of chronic myelogenous leukaemia (CML) by ima (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: AChE/ CD63/ Hsc70
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ Hsc70/ AChE
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Hsc70/ AChE
Characterization: Particle analysis
EM
EM-type
scanning EM
Image type
Wide-field
Report size (nm)
Not reported
|
||||||||
EV120120 | 1/2 | Homo sapiens | NAY | (d)(U)C | Corcoran C | 2012 | 11% | |
Study summaryFull title
All authors
Corcoran C, Rani S, O'Brien K, O'Neill A, Prencipe M, Sheikh R, Webb G, McDermott R, Watson W, Crown J, O'Driscoll L
Journal
PLoS One
Abstract
BACKGROUND: Hormone-refractory prostate cancer remains hindered by inevitable progression of resista (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Alix/ TSG101/ GAPDH
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ TSG101/ GAPDH
ELISA
Antibody details provided?
No
Detected EV-associated proteins
GAPDH
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV120052 | 2/2 | Mus musculus | Spleen, lymph node and tumor suspensions |
(d)(U)C Filtration IAF |
Cai Z | 2012 | 11% | |
Study summaryFull title
All authors
Cai Z, Yang F, Yu L, Yu Z, Jiang L, Wang Q, Yang Y, Wang L, Cao X, Wang J
Journal
J Immunol
Abstract
Activated T cells release bioactive Fas ligand (FasL) in exosomes, which subsequently induce self-ap (show more...)
EV-METRIC
11% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Spleen, lymph node and tumor suspensions
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration IAF Protein markers
EV: TSG101/ HSP70/ Beta-actin
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Spleen, lymph node and tumor suspensions
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Filtration steps
0.22µm or 0.2µm
Immunoaffinity capture
Selected surface protein(s)
CD8
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
HSP70/ TSG101/ Beta-actin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Beta-actin
Characterization: Particle analysis
None
|
||||||||
EV210063 | 1/3 | Homo sapiens | Blood plasma | (d)(U)C | Lok, Christine A R | 2012 | 0% | |
Study summaryFull title
All authors
Christine A R Lok, Karin S Snijder, Rienk Nieuwland, Joris A M Van Der Post, Paul de Vos, Marijke M Faas
Journal
Am J Reprod Immunol
Abstract
Problem: Preeclampsia is a pregnancy-specific disorder that may result from an adverse maternal resp (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Non-pregnant
Focus vesicles
microparticle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: time(min)
30
Pelleting: rotor type
Not specified
Pelleting: speed (g)
18890
Wash: volume per pellet (ml)
500
Wash: time (min)
30
Wash: Rotor Type
Not specified
Wash: speed (g)
18890
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210063 | 2/3 | Homo sapiens | Blood plasma | (d)(U)C | Lok, Christine A R | 2012 | 0% | |
Study summaryFull title
All authors
Christine A R Lok, Karin S Snijder, Rienk Nieuwland, Joris A M Van Der Post, Paul de Vos, Marijke M Faas
Journal
Am J Reprod Immunol
Abstract
Problem: Preeclampsia is a pregnancy-specific disorder that may result from an adverse maternal resp (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Pregnant
Focus vesicles
microparticle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: time(min)
30
Pelleting: rotor type
Not specified
Pelleting: speed (g)
18890
Wash: volume per pellet (ml)
500
Wash: time (min)
30
Wash: Rotor Type
Not specified
Wash: speed (g)
18890
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210063 | 3/3 | Homo sapiens | Blood plasma | (d)(U)C | Lok, Christine A R | 2012 | 0% | |
Study summaryFull title
All authors
Christine A R Lok, Karin S Snijder, Rienk Nieuwland, Joris A M Van Der Post, Paul de Vos, Marijke M Faas
Journal
Am J Reprod Immunol
Abstract
Problem: Preeclampsia is a pregnancy-specific disorder that may result from an adverse maternal resp (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Pre-eclampsia
Focus vesicles
microparticle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: time(min)
30
Pelleting: rotor type
Not specified
Pelleting: speed (g)
18890
Wash: volume per pellet (ml)
500
Wash: time (min)
30
Wash: Rotor Type
Not specified
Wash: speed (g)
18890
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV120187 | 1/1 | Paracoccidioides brasiliensis | Yeast |
(d)(U)C UF |
Vallejo MC | 2012 | 0% | |
Study summaryFull title
All authors
Vallejo MC, Nakayasu ES, Longo LV, Ganiko L, Lopes FG, Matsuo AL, Almeida IC, Puccia R
Journal
PLoS One
Abstract
BACKGROUND: Fungal extracellular vesicles are able to cross the cell wall and transport molecules th (show more...)
EV-METRIC
0% (median: 20% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Yeast
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
UF Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Paracoccidioides brasiliensis
Sample Type
Yeast
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Characterization: Particle analysis
None
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