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You searched for: EV220119 (EV-TRACK ID)
Showing 1 - 14 of 14
Showing 1 - 14 of 14
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV220119 | 3/14 | Schistosoma mansoni | whole parasite culture |
(d)(U)C DG |
Kuipers, Marije | 2022 | 56% | |
Study summaryFull title
All authors
Marije E Kuipers, Roman I Koning, Erik Bos, Cornelis H Hokke, Hermelijn H Smits, Esther N M Nolte-'t Hoen
Journal
J immunol Res
Abstract
In the past decade, the interest in helminth-derived extracellular vesicles (EVs) increased owing to (show more...)
EV-METRIC
56% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
whole parasite culture
Sample origin
adult worm
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: S. mansoni TSP-2
non-EV: None Proteomics
no
EV density (g/ml)
1.07-1.15
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Schistosoma mansoni
Sample Type
whole parasite culture
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
96,808
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
10%
Highest density fraction
60% (58,7% with sample)
Total gradient volume, incl. sample (mL)
11.97
Sample volume (mL)
0.07
Orientation
Bottom-up
Rotor type
SW 41 Ti
Speed (g)
169,044
Duration (min)
>960
Fraction volume (mL)
0.997
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
S. mansoni TSP-2
Characterization: Lipid analysis
No
|
||||||||
EV220119 | 4/14 | Schistosoma mansoni | whole parasite culture |
(d)(U)C DG |
Kuipers, Marije | 2022 | 56% | |
Study summaryFull title
All authors
Marije E Kuipers, Roman I Koning, Erik Bos, Cornelis H Hokke, Hermelijn H Smits, Esther N M Nolte-'t Hoen
Journal
J immunol Res
Abstract
In the past decade, the interest in helminth-derived extracellular vesicles (EVs) increased owing to (show more...)
EV-METRIC
56% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
whole parasite culture
Sample origin
adult worm
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: S. mansoni TSP-2
non-EV: None Proteomics
no
EV density (g/ml)
1.07-1.21
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Schistosoma mansoni
Sample Type
whole parasite culture
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
96,808
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
10%
Highest density fraction
60% (56.4 % with sample)
Total gradient volume, incl. sample (mL)
4.25
Sample volume (mL)
0.07
Orientation
Bottom-up
Rotor type
SW 55 Ti
Speed (g)
169,639
Duration (min)
>960
Fraction volume (mL)
0.354
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
S. mansoni TSP-2
Characterization: Lipid analysis
No
|
||||||||
EV220119 | 5/14 | Schistosoma mansoni | whole parasite culture |
(d)(U)C DG |
Kuipers, Marije | 2022 | 56% | |
Study summaryFull title
All authors
Marije E Kuipers, Roman I Koning, Erik Bos, Cornelis H Hokke, Hermelijn H Smits, Esther N M Nolte-'t Hoen
Journal
J immunol Res
Abstract
In the past decade, the interest in helminth-derived extracellular vesicles (EVs) increased owing to (show more...)
EV-METRIC
56% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
whole parasite culture
Sample origin
adult worm
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: S. mansoni TSP-2
non-EV: None Proteomics
no
EV density (g/ml)
1.07-1.21
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Schistosoma mansoni
Sample Type
whole parasite culture
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
96,808
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
10%
Highest density fraction
60% (51.8 % with sample)
Total gradient volume, incl. sample (mL)
1.742
Sample volume (mL)
0.07
Orientation
Bottom-up
Rotor type
TLS-55
Speed (g)
166,180
Duration (min)
120
Fraction volume (mL)
0.145
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
S. mansoni TSP-2
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Cryo-EM
Image type
Wide-field
|
||||||||
EV220119 | 6/14 | Schistosoma mansoni | whole parasite culture |
(d)(U)C DG |
Kuipers, Marije | 2022 | 56% | |
Study summaryFull title
All authors
Marije E Kuipers, Roman I Koning, Erik Bos, Cornelis H Hokke, Hermelijn H Smits, Esther N M Nolte-'t Hoen
Journal
J immunol Res
Abstract
In the past decade, the interest in helminth-derived extracellular vesicles (EVs) increased owing to (show more...)
EV-METRIC
56% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
whole parasite culture
Sample origin
adult worm
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: S. mansoni TSP-2
non-EV: None Proteomics
no
EV density (g/ml)
1.07-1.19
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Schistosoma mansoni
Sample Type
whole parasite culture
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
96,808
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
10%
Highest density fraction
60%
Total gradient volume, incl. sample (mL)
1.742
Sample volume (mL)
0.07
Orientation
Top-down
Rotor type
TLS-55
Speed (g)
166,180
Duration (min)
120
Fraction volume (mL)
0.145
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
S. mansoni TSP-2
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220119 | 7/14 | Schistosoma mansoni | whole parasite culture |
(d)(U)C DG |
Kuipers, Marije | 2022 | 56% | |
Study summaryFull title
All authors
Marije E Kuipers, Roman I Koning, Erik Bos, Cornelis H Hokke, Hermelijn H Smits, Esther N M Nolte-'t Hoen
Journal
J immunol Res
Abstract
In the past decade, the interest in helminth-derived extracellular vesicles (EVs) increased owing to (show more...)
EV-METRIC
56% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
whole parasite culture
Sample origin
adult worm
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: S. mansoni TSP-2
non-EV: None Proteomics
no
EV density (g/ml)
1.25-1.11
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Schistosoma mansoni
Sample Type
whole parasite culture
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
96,808
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
15
Lowest density fraction
0.4M
Highest density fraction
2.5M (2.05M with sample)
Total gradient volume, incl. sample (mL)
1.86
Sample volume (mL)
0.07
Orientation
Bottom-up
Rotor type
TLS-55
Speed (g)
166,180
Duration (min)
120
Fraction volume (mL)
0.155
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
S. mansoni TSP-2
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220119 | 8/14 | Schistosoma mansoni | whole parasite culture |
(d)(U)C DG |
Kuipers, Marije | 2022 | 56% | |
Study summaryFull title
All authors
Marije E Kuipers, Roman I Koning, Erik Bos, Cornelis H Hokke, Hermelijn H Smits, Esther N M Nolte-'t Hoen
Journal
J immunol Res
Abstract
In the past decade, the interest in helminth-derived extracellular vesicles (EVs) increased owing to (show more...)
EV-METRIC
56% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
whole parasite culture
Sample origin
adult worm
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: S. mansoni TSP-2
non-EV: None Proteomics
no
EV density (g/ml)
1.20-1.12
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Schistosoma mansoni
Sample Type
whole parasite culture
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
96,808
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
15
Lowest density fraction
0.4M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
1.86
Sample volume (mL)
0.07
Orientation
Top-down
Rotor type
TLS-55
Speed (g)
166,180
Duration (min)
120
Fraction volume (mL)
0.155
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
S. mansoni TSP-2
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220119 | 9/14 | Schistosoma mansoni | whole parasite culture |
(d)(U)C DG |
Kuipers, Marije | 2022 | 56% | |
Study summaryFull title
All authors
Marije E Kuipers, Roman I Koning, Erik Bos, Cornelis H Hokke, Hermelijn H Smits, Esther N M Nolte-'t Hoen
Journal
J immunol Res
Abstract
In the past decade, the interest in helminth-derived extracellular vesicles (EVs) increased owing to (show more...)
EV-METRIC
56% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
whole parasite culture
Sample origin
adult worm
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: S. mansoni TSP-2
non-EV: None Proteomics
no
EV density (g/ml)
1.17-1.12
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Schistosoma mansoni
Sample Type
whole parasite culture
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
96,808
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
15
Lowest density fraction
0.4M
Highest density fraction
2.5M (2.05M with sample)
Total gradient volume, incl. sample (mL)
1.86
Sample volume (mL)
0.07
Orientation
Bottom-up
Rotor type
TLS-55
Speed (g)
166,180
Duration (min)
810
Fraction volume (mL)
0.155
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
S. mansoni TSP-2
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220119 | 11/14 | Schistosoma mansoni | whole parasite culture |
(d)(U)C DG |
Kuipers, Marije | 2022 | 56% | |
Study summaryFull title
All authors
Marije E Kuipers, Roman I Koning, Erik Bos, Cornelis H Hokke, Hermelijn H Smits, Esther N M Nolte-'t Hoen
Journal
J immunol Res
Abstract
In the past decade, the interest in helminth-derived extracellular vesicles (EVs) increased owing to (show more...)
EV-METRIC
56% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
whole parasite culture
Sample origin
schistosomula
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: S. mansoni TSP-2
non-EV: None Proteomics
no
EV density (g/ml)
1.07-1.21
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Schistosoma mansoni
Sample Type
whole parasite culture
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
96,808
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
10%
Highest density fraction
60% (51.8 % with sample)
Total gradient volume, incl. sample (mL)
1.742
Sample volume (mL)
0.07
Orientation
Bottom-up
Rotor type
TLS-55
Speed (g)
166,180
Duration (min)
120
Fraction volume (mL)
0.145
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
S. mansoni TSP-2
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220119 | 12/14 | Schistosoma mansoni | whole parasite culture |
(d)(U)C DG |
Kuipers, Marije | 2022 | 56% | |
Study summaryFull title
All authors
Marije E Kuipers, Roman I Koning, Erik Bos, Cornelis H Hokke, Hermelijn H Smits, Esther N M Nolte-'t Hoen
Journal
J immunol Res
Abstract
In the past decade, the interest in helminth-derived extracellular vesicles (EVs) increased owing to (show more...)
EV-METRIC
56% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
whole parasite culture
Sample origin
schistosomula
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: S. mansoni TSP-2
non-EV: None Proteomics
no
EV density (g/ml)
1.07-1.19
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Schistosoma mansoni
Sample Type
whole parasite culture
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
96,808
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
10%
Highest density fraction
60%
Total gradient volume, incl. sample (mL)
1.742
Sample volume (mL)
0.07
Orientation
Top-down
Rotor type
TLS-55
Speed (g)
166,180
Duration (min)
120
Fraction volume (mL)
0.145
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
S. mansoni TSP-2
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220119 | 13/14 | Schistosoma mansoni | whole parasite culture |
(d)(U)C DG |
Kuipers, Marije | 2022 | 56% | |
Study summaryFull title
All authors
Marije E Kuipers, Roman I Koning, Erik Bos, Cornelis H Hokke, Hermelijn H Smits, Esther N M Nolte-'t Hoen
Journal
J immunol Res
Abstract
In the past decade, the interest in helminth-derived extracellular vesicles (EVs) increased owing to (show more...)
EV-METRIC
56% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
whole parasite culture
Sample origin
schistosomula
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: S. mansoni TSP-2
non-EV: None Proteomics
no
EV density (g/ml)
1.26-1.25
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Schistosoma mansoni
Sample Type
whole parasite culture
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
96,808
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
15
Lowest density fraction
0.4M
Highest density fraction
2.5M (2.05M with sample)
Total gradient volume, incl. sample (mL)
1.86
Sample volume (mL)
0.07
Orientation
Bottom-up
Rotor type
TLS-55
Speed (g)
166,180
Duration (min)
810
Fraction volume (mL)
0.155
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
S. mansoni TSP-2
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220119 | 14/14 | Schistosoma mansoni | whole parasite culture |
(d)(U)C DG |
Kuipers, Marije | 2022 | 56% | |
Study summaryFull title
All authors
Marije E Kuipers, Roman I Koning, Erik Bos, Cornelis H Hokke, Hermelijn H Smits, Esther N M Nolte-'t Hoen
Journal
J immunol Res
Abstract
In the past decade, the interest in helminth-derived extracellular vesicles (EVs) increased owing to (show more...)
EV-METRIC
56% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
whole parasite culture
Sample origin
schistosomula
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: S. mansoni TSP-2
non-EV: None Proteomics
no
EV density (g/ml)
1.28-1.21
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Schistosoma mansoni
Sample Type
whole parasite culture
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
96,808
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
15
Lowest density fraction
0.4M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
1.86
Sample volume (mL)
0.07
Orientation
Top-down
Rotor type
TLS-55
Speed (g)
166,180
Duration (min)
810
Fraction volume (mL)
0.155
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
S. mansoni TSP-2
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220119 | 1/14 | Schistosoma mansoni | whole parasite culture | (d)(U)C | Kuipers, Marije | 2022 | 44% | |
Study summaryFull title
All authors
Marije E Kuipers, Roman I Koning, Erik Bos, Cornelis H Hokke, Hermelijn H Smits, Esther N M Nolte-'t Hoen
Journal
J immunol Res
Abstract
In the past decade, the interest in helminth-derived extracellular vesicles (EVs) increased owing to (show more...)
EV-METRIC
44% (12th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
whole parasite culture
Sample origin
adult worm
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: S. mansoni TSP-2
non-EV: None Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Schistosoma mansoni
Sample Type
whole parasite culture
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
96,808
Wash: volume per pellet (ml)
11
Wash: time (min)
65
Wash: Rotor Type
SW 41 Ti
Wash: speed (g)
96,808
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
S. mansoni TSP-2
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
Extra information
In some cases, 2 collected EV fractions from protocol 3 and 4 were pooled and subjected to a washing step to re-pellet the EVs. Washing UC step of fractions from protocol 3 were performed in SW32 (average 125,755 ×g) and fractions from protocol 4 in SW41 (average 126,444 ×g) for 65 minutes. In another case, the collected fractions from protocol 3, 4 and 5 were subjected to TCA. In addition, in one experiment, adult worm EVs obtained by protocol 5 were concentrated and washed by ultrafiltration (10kDa, regenerated cellulose). Finally, density gradient fractions from protocol 5-10 were directly mixed with sample buffer.
|
||||||||
EV220119 | 10/14 | Schistosoma mansoni | whole parasite culture | (d)(U)C | Kuipers, Marije | 2022 | 44% | |
Study summaryFull title
All authors
Marije E Kuipers, Roman I Koning, Erik Bos, Cornelis H Hokke, Hermelijn H Smits, Esther N M Nolte-'t Hoen
Journal
J immunol Res
Abstract
In the past decade, the interest in helminth-derived extracellular vesicles (EVs) increased owing to (show more...)
EV-METRIC
44% (12th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
whole parasite culture
Sample origin
schistosomula
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: S. mansoni TSP-2
non-EV: None Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Schistosoma mansoni
Sample Type
whole parasite culture
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
96,808
Wash: volume per pellet (ml)
11
Wash: time (min)
65
Wash: Rotor Type
SW 41 Ti
Wash: speed (g)
96,808
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
S. mansoni TSP-2
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV220119 | 2/14 | Schistosoma mansoni | whole parasite culture |
(d)(U)C UF qEV |
Kuipers, Marije | 2022 | 25% | |
Study summaryFull title
All authors
Marije E Kuipers, Roman I Koning, Erik Bos, Cornelis H Hokke, Hermelijn H Smits, Esther N M Nolte-'t Hoen
Journal
J immunol Res
Abstract
In the past decade, the interest in helminth-derived extracellular vesicles (EVs) increased owing to (show more...)
EV-METRIC
25% (3 percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
whole parasite culture
Sample origin
adult worm
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Ultrafiltration qEV Protein markers
EV: S. mansoni TSP-2
non-EV: None Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Schistosoma mansoni
Sample Type
whole parasite culture
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Ultra filtration
Cut-off size (kDa)
10 & 3
Membrane type
Regenerated cellulose
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
S. mansoni TSP-2
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
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1 - 14 of 14 |
EV-TRACK ID | EV220119 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
species | Schistosoma mansoni | |||||||||||||
sample type | whole parasite culture | |||||||||||||
condition | adult worm | adult worm | adult worm | adult worm | adult worm | adult worm | adult worm | schistosomula | schistosomula | schistosomula | schistosomula | adult worm | schistosomula | adult worm |
separation protocol | dUC/ Density gradient | dUC/ Density gradient | dUC/ Density gradient | dUC/ Density gradient | dUC/ Density gradient | dUC/ Density gradient | dUC/ Density gradient | dUC/ Density gradient | dUC/ Density gradient | dUC/ Density gradient | dUC/ Density gradient | dUC | dUC | dUC/ Ultrafiltration/ qEV |
Exp. nr. | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 11 | 12 | 13 | 14 | 1 | 10 | 2 |
EV-METRIC % | 56 | 56 | 56 | 56 | 56 | 56 | 56 | 56 | 56 | 56 | 56 | 44 | 44 | 25 |