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You searched for: EV220069 (EV-TRACK ID)
Showing 1 - 11 of 11
Showing 1 - 11 of 11
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV220069 | 1/11 | Homo sapiens | MHCC97L | (d)(U)C | Tey, Sze Kong | 2022 | 56% | |
Study summaryFull title
All authors
Sze Keong Tey, Samuel Wan Ki Wong, Cherlie Lot Sum Yeung, Jason Ying Ki Li, Xiaowen Mao, Clive Yik Sham Chung, Judy Wai Ping Yam
Journal
Journal of Extracellular Biology
Abstract
MET receptor tyrosine kinase is a cell surface receptor that plays important role in embryonic devel (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ CD9/ TSG101
non-EV: GM130/ tubulin-alpha/ Calreticulin/ Albumin/ Argonaute2/ PMP70/ Prohibitin/ Tamm-Horsfall protein Proteomics
yes
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MHCC97L
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
70
Wash: time (min)
70
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ CD9/ TSG101
Not detected contaminants
GM130/ tubulin-alpha
Proteomics database
ProteomeXchange
Detected contaminants
Calreticulin
Not detected contaminants
Albumin/ Argonaute2/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
156.5
EM
EM-type
Immuno-EM
EM protein
CD63
Image type
Close-up
|
||||||||
EV220069 | 2/11 | Homo sapiens | MHCC97L | (d)(U)C | Tey, Sze Kong | 2022 | 56% | |
Study summaryFull title
All authors
Sze Keong Tey, Samuel Wan Ki Wong, Cherlie Lot Sum Yeung, Jason Ying Ki Li, Xiaowen Mao, Clive Yik Sham Chung, Judy Wai Ping Yam
Journal
Journal of Extracellular Biology
Abstract
MET receptor tyrosine kinase is a cell surface receptor that plays important role in embryonic devel (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
nMet overexpressing
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ CD9/ TSG101
non-EV: GM130/ tubulin-alpha/ Calreticulin/ Albumin/ Argonaute2/ PMP70/ Prohibitin/ Tamm-Horsfall protein Proteomics
yes
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MHCC97L
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
70
Wash: time (min)
70
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ CD9/ TSG101
Not detected contaminants
GM130/ tubulin-alpha
Proteomics database
ProteomeXchange
Detected contaminants
Calreticulin
Not detected contaminants
Albumin/ Argonaute2/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
155.1
EM
EM-type
Immuno-EM
EM protein
CD63
Image type
Close-up
|
||||||||
EV220069 | 3/11 | Homo sapiens | MHCC97L | (d)(U)C | Tey, Sze Kong | 2022 | 44% | |
Study summaryFull title
All authors
Sze Keong Tey, Samuel Wan Ki Wong, Cherlie Lot Sum Yeung, Jason Ying Ki Li, Xiaowen Mao, Clive Yik Sham Chung, Judy Wai Ping Yam
Journal
Journal of Extracellular Biology
Abstract
MET receptor tyrosine kinase is a cell surface receptor that plays important role in embryonic devel (show more...)
EV-METRIC
44% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
nMet overexpressing/ shControl
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ TSG101
non-EV: GM130/ tubulin-alpha Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MHCC97L
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
70
Wash: time (min)
70
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ TSG101
Not detected contaminants
GM130/ tubulin-alpha
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
158.1
EM
EM-type
Immuno-EM
EM protein
CD63
Image type
Close-up
|
||||||||
EV220069 | 4/11 | Homo sapiens | MHCC97L | (d)(U)C | Tey, Sze Kong | 2022 | 44% | |
Study summaryFull title
All authors
Sze Keong Tey, Samuel Wan Ki Wong, Cherlie Lot Sum Yeung, Jason Ying Ki Li, Xiaowen Mao, Clive Yik Sham Chung, Judy Wai Ping Yam
Journal
Journal of Extracellular Biology
Abstract
MET receptor tyrosine kinase is a cell surface receptor that plays important role in embryonic devel (show more...)
EV-METRIC
44% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
nMet overexpressing/ shEIF3I-1
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ TSG101
non-EV: GM130/ tubulin-alpha Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MHCC97L
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
70
Wash: time (min)
70
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ TSG101
Not detected contaminants
GM130/ tubulin-alpha
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
151.6
EM
EM-type
Immuno-EM
EM protein
CD63
Image type
Close-up
|
||||||||
EV220069 | 5/11 | Homo sapiens | MHCC97L | (d)(U)C | Tey, Sze Kong | 2022 | 44% | |
Study summaryFull title
All authors
Sze Keong Tey, Samuel Wan Ki Wong, Cherlie Lot Sum Yeung, Jason Ying Ki Li, Xiaowen Mao, Clive Yik Sham Chung, Judy Wai Ping Yam
Journal
Journal of Extracellular Biology
Abstract
MET receptor tyrosine kinase is a cell surface receptor that plays important role in embryonic devel (show more...)
EV-METRIC
44% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
nMet overexpressing/ shEIF3I-2
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ TSG101
non-EV: GM130/ tubulin-alpha Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MHCC97L
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
70
Wash: time (min)
70
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ TSG101
Not detected contaminants
GM130/ tubulin-alpha
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
158.7
EM
EM-type
Immuno-EM
EM protein
CD63
Image type
Close-up
|
||||||||
EV220069 | 6/11 | Homo sapiens | MHCC97L | (d)(U)C | Tey, Sze Kong | 2022 | 44% | |
Study summaryFull title
All authors
Sze Keong Tey, Samuel Wan Ki Wong, Cherlie Lot Sum Yeung, Jason Ying Ki Li, Xiaowen Mao, Clive Yik Sham Chung, Judy Wai Ping Yam
Journal
Journal of Extracellular Biology
Abstract
MET receptor tyrosine kinase is a cell surface receptor that plays important role in embryonic devel (show more...)
EV-METRIC
44% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
nMet overexpressing/ shEIF4G2-1
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ TSG101
non-EV: GM130/ tubulin-alpha Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MHCC97L
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
70
Wash: time (min)
70
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ TSG101
Not detected contaminants
GM130/ tubulin-alpha
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
157.7
EM
EM-type
Immuno-EM
EM protein
CD63
Image type
Close-up
|
||||||||
EV220069 | 7/11 | Homo sapiens | MHCC97L | (d)(U)C | Tey, Sze Kong | 2022 | 44% | |
Study summaryFull title
All authors
Sze Keong Tey, Samuel Wan Ki Wong, Cherlie Lot Sum Yeung, Jason Ying Ki Li, Xiaowen Mao, Clive Yik Sham Chung, Judy Wai Ping Yam
Journal
Journal of Extracellular Biology
Abstract
MET receptor tyrosine kinase is a cell surface receptor that plays important role in embryonic devel (show more...)
EV-METRIC
44% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
nMet overexpressing/ shEIF4G2-2
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ TSG101
non-EV: GM130/ tubulin-alpha Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MHCC97L
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
70
Wash: time (min)
70
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ TSG101
Not detected contaminants
GM130/ tubulin-alpha
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
160.3
EM
EM-type
Immuno-EM
EM protein
CD63
Image type
Close-up
|
||||||||
EV220069 | 8/11 | Homo sapiens | MHCC97L | (d)(U)C | Tey, Sze Kong | 2022 | 44% | |
Study summaryFull title
All authors
Sze Keong Tey, Samuel Wan Ki Wong, Cherlie Lot Sum Yeung, Jason Ying Ki Li, Xiaowen Mao, Clive Yik Sham Chung, Judy Wai Ping Yam
Journal
Journal of Extracellular Biology
Abstract
MET receptor tyrosine kinase is a cell surface receptor that plays important role in embryonic devel (show more...)
EV-METRIC
44% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
nMet overexpressing/ shRPS3A-1
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ TSG101
non-EV: GM130/ tubulin-alpha Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MHCC97L
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
70
Wash: time (min)
70
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ TSG101
Not detected contaminants
GM130/ tubulin-alpha
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
154.3
EM
EM-type
Immuno-EM
EM protein
CD63
Image type
Close-up
|
||||||||
EV220069 | 9/11 | Homo sapiens | MHCC97L | (d)(U)C | Tey, Sze Kong | 2022 | 44% | |
Study summaryFull title
All authors
Sze Keong Tey, Samuel Wan Ki Wong, Cherlie Lot Sum Yeung, Jason Ying Ki Li, Xiaowen Mao, Clive Yik Sham Chung, Judy Wai Ping Yam
Journal
Journal of Extracellular Biology
Abstract
MET receptor tyrosine kinase is a cell surface receptor that plays important role in embryonic devel (show more...)
EV-METRIC
44% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
nMet overexpressing/ shRPS3A-2
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ TSG101
non-EV: GM130/ tubulin-alpha Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MHCC97L
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
70
Wash: time (min)
70
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ TSG101
Not detected contaminants
GM130/ tubulin-alpha
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
159.6
EM
EM-type
Immuno-EM
EM protein
CD63
Image type
Close-up
|
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EV220069 | 10/11 | Homo sapiens | MHCC97L | (d)(U)C | Tey, Sze Kong | 2022 | 44% | |
Study summaryFull title
All authors
Sze Keong Tey, Samuel Wan Ki Wong, Cherlie Lot Sum Yeung, Jason Ying Ki Li, Xiaowen Mao, Clive Yik Sham Chung, Judy Wai Ping Yam
Journal
Journal of Extracellular Biology
Abstract
MET receptor tyrosine kinase is a cell surface receptor that plays important role in embryonic devel (show more...)
EV-METRIC
44% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
nMet overexpressing/ shRPL10-1
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ TSG101
non-EV: GM130/ tubulin-alpha Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MHCC97L
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
70
Wash: time (min)
70
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ TSG101
Not detected contaminants
GM130/ tubulin-alpha
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
159.1
EM
EM-type
Immuno-EM
EM protein
CD63
Image type
Close-up
|
||||||||
EV220069 | 11/11 | Homo sapiens | MHCC97L | (d)(U)C | Tey, Sze Kong | 2022 | 44% | |
Study summaryFull title
All authors
Sze Keong Tey, Samuel Wan Ki Wong, Cherlie Lot Sum Yeung, Jason Ying Ki Li, Xiaowen Mao, Clive Yik Sham Chung, Judy Wai Ping Yam
Journal
Journal of Extracellular Biology
Abstract
MET receptor tyrosine kinase is a cell surface receptor that plays important role in embryonic devel (show more...)
EV-METRIC
44% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
nMet overexpressing/ shRPL10-2
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ TSG101
non-EV: GM130/ tubulin-alpha Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MHCC97L
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
70
Wash: time (min)
70
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ TSG101
Not detected contaminants
GM130/ tubulin-alpha
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
158.5
EM
EM-type
Immuno-EM
EM protein
CD63
Image type
Close-up
|
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1 - 11 of 11 |
EV-TRACK ID | EV220069 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
species | Homo sapiens | ||||||||||
sample type | Cell culture | ||||||||||
cell type | MHCC97L | ||||||||||
condition | Control condition | nMet overexpressing | nMet overexpressing/ shControl | nMet overexpressing/ shEIF3I-1 | nMet overexpressing/ shEIF3I-2 | nMet overexpressing/ shEIF4G2-1 | nMet overexpressing/ shEIF4G2-2 | nMet overexpressing/ shRPS3A-1 | nMet overexpressing/ shRPS3A-2 | nMet overexpressing/ shRPL10-1 | nMet overexpressing/ shRPL10-2 |
separation protocol | dUC | dUC | dUC | dUC | dUC | dUC | dUC | dUC | dUC | dUC | dUC |
Exp. nr. | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 |
EV-METRIC % | 56 | 56 | 44 | 44 | 44 | 44 | 44 | 44 | 44 | 44 | 44 |