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You searched for: EV210251 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210251 | 1/1 | Sus scrofa | Follicular fluid | Exo-spin | Gad A | 2022 | 75% | |
Study summaryFull title
All authors
Gad A, Murin M, Bartkova A, Kinterova V, Marcollova K, Laurincik J, Prochazka R
Journal
J Anim Sci Biotechnol
Abstract
Ovarian follicular fluids (FFs) contain several kinds of regulatory factors that maintain a suitable (show more...)
EV-METRIC
75% (95th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Follicular fluid
Sample origin
Control condition
Focus vesicles
small extracellular vesicle
Separation protocol
Separation protocol
Commercial method
Protein markers
EV: Alix/ CD63/ TSG101
non-EV: ATP5A/ CytC Proteomics
no
Show all info
Study aim
Biomarker/Identification of content (omics approaches)
Sample
Species
Sus scrofa
Sample Type
Follicular fluid
Separation Method
Commercial kit
Exo-spin
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
particles per milliliter of starting sample
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ CD63/ TSG101
Not detected contaminants
ATP5A/ CytC
Characterization: RNA analysis
RNA analysis
Type
Capillary electrophoresis (e.g. Bioanalyzer)
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Median
Reported size (nm)
135
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 9.00e+9
EM
EM-type
Transmission EM
Image type
Close-up, Wide-field
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1 - 1 of 1 |
EV-TRACK ID | EV210251 |
---|---|
species | Sus scrofa |
sample type | Follicular fluid |
condition | Control condition |
separation protocol | Exo-spin |
Exp. nr. | 1 |
EV-METRIC % | 75 |