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You searched for: EV210210 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210210 | 1/1 | Homo sapiens | Expi293F |
(d)(U)C Tangential flow filtration qEV10 Filtration UF |
Driedonks T | 2022 | 63% | |
Study summaryFull title
All authors
Driedonks T, Jiang L, Carlson B, Han Z, Liu G, Queen SE, Shirk EN, Gololobova O, Liao Z, Nyberg LH, Lima G, Paniushkina L, Garcia-Contreras M, Schonvisky K, Castell N, Stover M, Guerrero-Martin S, Richardson R, Smith B, Machairaki V, Lai CP, Izzi JM, Hutchinson EK, Pate KAM, Witwer KW
Journal
J Extracell Biol
Abstract
Extracellular vesicles (EVs) have potential in disease treatment since they can be loaded with thera (show more...)
EV-METRIC
63% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
pLenti-palmGRET (Addgene #158221) transient expression
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Tangential flow filtration Commercial method Filtration Ultrafiltration Protein markers
EV: CD9/ CD63/ CD81/ TSG101/ CD29/ CD146/ CD326/ CD44/ ROR1/ CD24
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Biodistribution/pharmacokinetics/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Expi293F
Cell count
3.30E+09
Separation Method
Filtration steps
0.2 or 0.22 µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Regenerated cellulose
Commercial kit
qEV10
Other
Name other separation method
Tangential flow filtration
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ TSG101/ CD47
Not detected contaminants
Calnexin
Other 1
SP-IRIS (Exoview)
Detected EV-associated proteins
CD63/ CD81
Other 2
MACSPlex
Detected EV-associated proteins
CD9/ CD63/ CD81/ CD29/ CD146/ CD326/ CD44/ ROR1/ CD24
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
122.6
EV concentration
Yes
Particle analysis: flow cytometry
Flow cytometer type
Amnis ImageStream MKII
Hardware adjustment
EM
EM-type
Transmission-EM
Image type
Wide-field
Extra information
Size-exclusion chromatography details: Izon qEV10, first 20 ml after the void fraction were considered EV-enriched fractions/ Tangential Flow Filtration: Sartorius Vivaflow 50R cassettes, 100 kD
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EV-TRACK ID | EV210210 |
---|---|
species | Homo sapiens |
sample type | Cell culture |
cell type | Expi293F |
condition | pLenti-palmGRET (Addgene #158221) transient expression |
separation protocol | dUC/ Tangential flow filtration/ qEV10/ Filtration/ Ultrafiltration |
Exp. nr. | 1 |
EV-METRIC % | 63 |