Search > Results
You searched for: EV210161 (EV-TRACK ID)
Showing 1 - 1 of 1
Showing 1 - 1 of 1
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210161 | 1/1 | Homo sapiens | HEK-293T |
(d)(U)C Filtration |
Fu, Yuxuan | 2021 | 44% | |
Study summaryFull title
All authors
Yuxuan Fu, Sidong Xiong
Journal
J Control Release
Abstract
The worldwide spread of COVID-19 highlights the urgent need for an efficient approach to rapidly dev (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: Alix/ CD9
non-EV: GM130/ Calreticulin Proteomics
no
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HEK-293T
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
50
Wash: time (min)
70
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ Alix
Not detected contaminants
Calreticulin/ GM130
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
128,1
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 360000000
|
||||||||
1 - 1 of 1 |
EV-TRACK ID | EV210161 |
---|---|
species | Homo sapiens |
sample type | Cell culture |
cell type | HEK-293T |
condition | Control condition |
separation protocol | (d)(U)C Filtration |
Exp. nr. | 1 |
EV-METRIC % | 44 |