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You searched for: EV210160 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210160 | 1/3 | Rattus norvegicus | Blood plasma |
(d)(U)C Other;ExoEasy Maxi Kit, Qiagen, Hilden, Germany |
Xu, Kaiyuan | 2021 | 38% | |
Study summaryFull title
Altered protein profile of plasma extracellular vesicles in oral squamous cell carcinoma development
All authors
Kaiyuan Xu, Liu Liu, Kaihui Wu, Miaomiao Zhang, Ruiqi Xie, Ruowei Li, Maomao Zhao, Hui Yang, Ning Duan, Xiang Wang, Wenmei Wang
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) are involved in a wide range of pathological processes and recognized a (show more...)
EV-METRIC
38% (72nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Commercial method Protein markers
EV: TSG101/ CD63/ CD9
non-EV: None Proteomics
yes
Show all info
Study aim
Function/Biomarker/Identification of content (omics approaches)
Sample
Species
Rattus norvegicus
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Commercial kit
Other;ExoEasy Maxi Kit, Qiagen, Hilden, Germany
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ TSG101
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
196,6
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV210160 | 2/3 | Rattus norvegicus | Blood plasma |
(d)(U)C Other;ExoEasy Maxi Kit, Qiagen, Hilden, Germany |
Xu, Kaiyuan | 2021 | 38% | |
Study summaryFull title
Altered protein profile of plasma extracellular vesicles in oral squamous cell carcinoma development
All authors
Kaiyuan Xu, Liu Liu, Kaihui Wu, Miaomiao Zhang, Ruiqi Xie, Ruowei Li, Maomao Zhao, Hui Yang, Ning Duan, Xiang Wang, Wenmei Wang
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) are involved in a wide range of pathological processes and recognized a (show more...)
EV-METRIC
38% (72nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
moderate dysplasia in tongue
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Commercial method Protein markers
EV: TSG101/ CD63/ CD9
non-EV: None Proteomics
yes
Show all info
Study aim
Function/Biomarker/Identification of content (omics approaches)
Sample
Species
Rattus norvegicus
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Commercial kit
Other;ExoEasy Maxi Kit, Qiagen, Hilden, Germany
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ TSG101
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210160 | 3/3 | Rattus norvegicus | Blood plasma |
(d)(U)C Other;ExoEasy Maxi Kit, Qiagen, Hilden, Germany |
Xu, Kaiyuan | 2021 | 38% | |
Study summaryFull title
Altered protein profile of plasma extracellular vesicles in oral squamous cell carcinoma development
All authors
Kaiyuan Xu, Liu Liu, Kaihui Wu, Miaomiao Zhang, Ruiqi Xie, Ruowei Li, Maomao Zhao, Hui Yang, Ning Duan, Xiang Wang, Wenmei Wang
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) are involved in a wide range of pathological processes and recognized a (show more...)
EV-METRIC
38% (72nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
oral squamous cell carcinoma in tongue
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Commercial method Protein markers
EV: TSG101/ CD63/ CD9
non-EV: None Proteomics
yes
Show all info
Study aim
Function/Biomarker/Identification of content (omics approaches)
Sample
Species
Rattus norvegicus
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Commercial kit
Other;ExoEasy Maxi Kit, Qiagen, Hilden, Germany
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ TSG101
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
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1 - 3 of 3 |
EV-TRACK ID | EV210160 | ||
---|---|---|---|
species | Rattus norvegicus | ||
sample type | Blood plasma | ||
condition | Control condition | moderate dysplasia in tongue | oral squamous cell carcinoma in tongue |
separation protocol | (d)(U)C Other ExoEasy Maxi Kit Qiagen Hilden Germany | (d)(U)C Other ExoEasy Maxi Kit Qiagen Hilden Germany | (d)(U)C Other ExoEasy Maxi Kit Qiagen Hilden Germany |
Exp. nr. | 1 | 2 | 3 |
EV-METRIC % | 38 | 38 | 38 |