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You searched for: EV210080 (EV-TRACK ID)

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Experiment number
  • If needed, multiple experiments were identified in a single publication based on differing sample types, separation protocols and/or vesicle types of interest.
Species
  • Species of origin of the EVs.
Separation protocol
  • Gives a short, non-chronological overview of the different steps of the separation protocol.
    • (d)(U)C = (differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
Details EV-TRACK ID Experiment nr. Species Sample type Separation protocol First author Year EV-METRIC
EV210080 1/2 Homo sapiens Serum (d)(U)C
ExoQuick
Shen, Rong-Kai 2016 25%

Study summary

Full title
All authors
Rong-Kai Shen, Xia Zhu, Huan Yi, Chao-Yang Wu, Fei Chen, Li-Qun Dai, Jian-Hua Lin
Journal
Int J Clin Exp Pathol
Abstract
http://www.ijcep.com/files/ijcep0019652.pdf Osteosarcoma (OS) is the most common primary bone tumo (show more...)http://www.ijcep.com/files/ijcep0019652.pdf Osteosarcoma (OS) is the most common primary bone tumor in children and adolescents. Combined therapy has not improved the prognosis of osteosarcoma in the past five years, while new strategies need to be explored. We extracted serum exosomes from OS and healthy people. Contrasting to exosomes from healthy ones, those derived from patients suffering from OS significantly promoted the ability of adhesion, migration and viability of the MG63 in vitro study. Osteosarcoma-derived exosomes package proteins were mainly intracellular ones, while these proteins exclusively expressed glucose-6-phospate dehydrogenase (G6PD), phosphofructokinase, transaldolase 1 and transketolase that reprogrammed tumor metabolism and prompted progression of osteosarcoma. Western-blot assay confirmed osteosarcoma-derived exosomes contained generous G6PD than health people. In conclusion, serum exosomal proteins originated from osteosarcoma and healthy people were quite different from each other. Exosomes derived from osteosarcoma could promote tumor progression, for G6PD were largely packaged by serum exosomes. These special serum exosomes, which are abundant in G6PD, can be a promising target for diagnostic and therapeutic strategy for osteosarcoma. (hide)
EV-METRIC
25% (67th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Serum
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(d)(U)C
Commercial method
Protein markers
EV: CD63/ Actin/ G6PD
non-EV: None
Proteomics
yes
Show all info
Study aim
Function/Biomarker/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Commercial kit
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ Actin/ G6PD
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
30-100
EV210080 2/2 Homo sapiens Serum (d)(U)C
ExoQuick
Shen, Rong-Kai 2016 25%

Study summary

Full title
All authors
Rong-Kai Shen, Xia Zhu, Huan Yi, Chao-Yang Wu, Fei Chen, Li-Qun Dai, Jian-Hua Lin
Journal
Int J Clin Exp Pathol
Abstract
http://www.ijcep.com/files/ijcep0019652.pdf Osteosarcoma (OS) is the most common primary bone tumo (show more...)http://www.ijcep.com/files/ijcep0019652.pdf Osteosarcoma (OS) is the most common primary bone tumor in children and adolescents. Combined therapy has not improved the prognosis of osteosarcoma in the past five years, while new strategies need to be explored. We extracted serum exosomes from OS and healthy people. Contrasting to exosomes from healthy ones, those derived from patients suffering from OS significantly promoted the ability of adhesion, migration and viability of the MG63 in vitro study. Osteosarcoma-derived exosomes package proteins were mainly intracellular ones, while these proteins exclusively expressed glucose-6-phospate dehydrogenase (G6PD), phosphofructokinase, transaldolase 1 and transketolase that reprogrammed tumor metabolism and prompted progression of osteosarcoma. Western-blot assay confirmed osteosarcoma-derived exosomes contained generous G6PD than health people. In conclusion, serum exosomal proteins originated from osteosarcoma and healthy people were quite different from each other. Exosomes derived from osteosarcoma could promote tumor progression, for G6PD were largely packaged by serum exosomes. These special serum exosomes, which are abundant in G6PD, can be a promising target for diagnostic and therapeutic strategy for osteosarcoma. (hide)
EV-METRIC
25% (67th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Serum
Sample origin
Osteosarcoma
Focus vesicles
exosome
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(d)(U)C
Commercial method
Protein markers
EV: CD63/ Actin/ G6PD
non-EV: None
Proteomics
yes
Show all info
Study aim
Function/Biomarker/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Commercial kit
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ Actin/ G6PD
Proteomics database
No
Characterization: Lipid analysis
No
1 - 2 of 2
  • CM = Commercial method
  • dUC = differential ultracentrifugation
  • DG = density gradient
  • UF = ultrafiltration
  • SEC = size-exclusion chromatography
EV-TRACK ID
EV210080
species
Homo sapiens
sample type
Serum
condition
Control condition
Osteosarcoma
separation protocol
(d)(U)C
ExoQuick
(d)(U)C
ExoQuick
Exp. nr.
1
2
EV-METRIC %
25
25