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You searched for: EV210078 (EV-TRACK ID)
Showing 1 - 5 of 5
Showing 1 - 5 of 5
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210078 | 1/5 | Homo sapiens | KHOS |
(d)(U)C Filtration ExoQuick |
Macklin, Rebecca | 2016 | 38% | |
Study summaryFull title
All authors
Rebecca Macklin, Haolu Wang, Dorothy Loo, Sally Martin, Andrew Cumming, Na Cai, Rebecca Lane, Natalia Saenz Ponce, Eleni Topkas, Kerry Inder, Nicholas A Saunders, Liliana Endo-Munoz
Journal
Oncotarget
Abstract
Osteosarcoma (OS) is the most common pediatric bone tumor and is associated with the emergence of pu (show more...)
EV-METRIC
38% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
HiMet-C6
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Commercial method Protein markers
EV: CD81/ HSP70/ CD63
non-EV: None Proteomics
yes
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
KHOS
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Overnight (16h) at >=100,000g + 0.2 µm filtration
Cell count
Not specified
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ HSP70
Not detected EV-associated proteins
CD81
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Modus
Reported size (nm)
85
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV210078 | 3/5 | Homo sapiens | KHOS |
(d)(U)C Filtration ExoQuick |
Macklin, Rebecca | 2016 | 25% | |
Study summaryFull title
All authors
Rebecca Macklin, Haolu Wang, Dorothy Loo, Sally Martin, Andrew Cumming, Na Cai, Rebecca Lane, Natalia Saenz Ponce, Eleni Topkas, Kerry Inder, Nicholas A Saunders, Liliana Endo-Munoz
Journal
Oncotarget
Abstract
Osteosarcoma (OS) is the most common pediatric bone tumor and is associated with the emergence of pu (show more...)
EV-METRIC
25% (64th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Commercial method Protein markers
EV: CD81/ HSP70/ CD63
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
KHOS
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Overnight (16h) at >=100,000g + 0.2 µm filtration
Cell count
Not specified
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ HSP70/ CD81
Characterization: Lipid analysis
No
|
||||||||
EV210078 | 4/5 | Homo sapiens | KHOS |
(d)(U)C Filtration ExoQuick |
Macklin, Rebecca | 2016 | 25% | |
Study summaryFull title
All authors
Rebecca Macklin, Haolu Wang, Dorothy Loo, Sally Martin, Andrew Cumming, Na Cai, Rebecca Lane, Natalia Saenz Ponce, Eleni Topkas, Kerry Inder, Nicholas A Saunders, Liliana Endo-Munoz
Journal
Oncotarget
Abstract
Osteosarcoma (OS) is the most common pediatric bone tumor and is associated with the emergence of pu (show more...)
EV-METRIC
25% (64th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
LoMet-C4
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Commercial method Protein markers
EV: CD81/ HSP70/ CD63
non-EV: None Proteomics
yes
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
KHOS
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Overnight (16h) at >=100,000g + 0.2 µm filtration
Cell count
Not specified
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ HSP70/ CD81
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Modus
Reported size (nm)
99
|
||||||||
EV210078 | 5/5 | Homo sapiens | HOS |
(d)(U)C Filtration ExoQuick |
Macklin, Rebecca | 2016 | 25% | |
Study summaryFull title
All authors
Rebecca Macklin, Haolu Wang, Dorothy Loo, Sally Martin, Andrew Cumming, Na Cai, Rebecca Lane, Natalia Saenz Ponce, Eleni Topkas, Kerry Inder, Nicholas A Saunders, Liliana Endo-Munoz
Journal
Oncotarget
Abstract
Osteosarcoma (OS) is the most common pediatric bone tumor and is associated with the emergence of pu (show more...)
EV-METRIC
25% (64th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Commercial method Protein markers
EV: CD81/ HSP70/ CD63
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HOS
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Overnight (16h) at >=100,000g + 0.2 µm filtration
Cell count
Not specified
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81
Not detected EV-associated proteins
HSP70/
Characterization: Lipid analysis
No
|
||||||||
EV210078 | 2/5 | Homo sapiens | KHOS |
(d)(U)C Filtration UF |
Macklin, Rebecca | 2016 | 0% | |
Study summaryFull title
All authors
Rebecca Macklin, Haolu Wang, Dorothy Loo, Sally Martin, Andrew Cumming, Na Cai, Rebecca Lane, Natalia Saenz Ponce, Eleni Topkas, Kerry Inder, Nicholas A Saunders, Liliana Endo-Munoz
Journal
Oncotarget
Abstract
Osteosarcoma (OS) is the most common pediatric bone tumor and is associated with the emergence of pu (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
HiMet-C6
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
KHOS
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Overnight (16h) at >=100,000g + 0.2 µm filtration
Cell count
Not specified
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100 000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
70
Wash: Rotor Type
Not specified
Wash: speed (g)
100 000
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Not specified
Protein Concentration Method
Bradford
Characterization: Lipid analysis
No
|
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1 - 5 of 5 |
EV-TRACK ID | EV210078 | ||||
---|---|---|---|---|---|
species | Homo sapiens | ||||
sample type | Cell culture | ||||
cell type | KHOS | KHOS | KHOS | HOS | KHOS |
condition | HiMet-C6 | Control condition | LoMet-C4 | Control condition | HiMet-C6 |
separation protocol | (d)(U)C Filtration ExoQuick | (d)(U)C Filtration ExoQuick | (d)(U)C Filtration ExoQuick | (d)(U)C Filtration ExoQuick | (d)(U)C Filtration UF |
Exp. nr. | 1 | 3 | 4 | 5 | 2 |
EV-METRIC % | 38 | 25 | 25 | 25 | 0 |