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You searched for: EV210056 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210056 | 1/2 | Mus musculus | WEHI3B |
DG (d)(U)C |
Gu, Xiaoyu | 2015 | 25% | |
Study summaryFull title
All authors
Xiaoyu Gu, Ulrike Erb, Markus W Büchler, Margot Zöller
Journal
Int J Cancer
Abstract
Leukemia immunotherapy frequently does not meet expectation, one of the handicaps being tumor exosom (show more...)
EV-METRIC
25% (64th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: CD24/ CD34/ CD90/ CD117/ SCA1/ CD16/32/ CD31/ CD44/ CD9/ CD63/ CD81/ MFGE8/ HSP70/ IL3/ IL6/ IL7/ CCR9/ CXCR3/ CXCR4
non-EV: None Proteomics
no
EV density (g/ml)
Not specified
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
WEHI3B
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
90
Wash: Rotor Type
Not specified
Wash: speed (g)
100000
Density gradient
Type
Continuous
Lowest density fraction
0.25M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
Not specified
Sample volume (mL)
Not spec
Orientation
Bottom-up
Rotor type
Not specified
Speed (g)
150000
Duration (min)
900
Fraction volume (mL)
Not specified
Fraction processing
Not specified
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry aspecific beads
Antibody details provided?
No
Detected EV-associated proteins
CD24/ CD34/ CD90/ CD117/ SCA1/ CD16/32/ CD31/ CD44/ CD9/ CD63/ CD81/ MFGE8/ HSP70/ IL3/ IL6/ IL7/ CCR9/ CXCR3/ CXCR4
Characterization: Lipid analysis
No
|
||||||||
EV210056 | 2/2 | Mus musculus | RENCA |
DG (d)(U)C |
Gu, Xiaoyu | 2015 | 14% | |
Study summaryFull title
All authors
Xiaoyu Gu, Ulrike Erb, Markus W Büchler, Margot Zöller
Journal
Int J Cancer
Abstract
Leukemia immunotherapy frequently does not meet expectation, one of the handicaps being tumor exosom (show more...)
EV-METRIC
14% (44th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: None
non-EV: None Proteomics
no
EV density (g/ml)
Not specified
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
RENCA
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
90
Wash: Rotor Type
Not specified
Wash: speed (g)
100000
Density gradient
Type
Continuous
Lowest density fraction
0.25M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
Not specified
Sample volume (mL)
Not spec
Orientation
Bottom-up
Rotor type
Not specified
Speed (g)
150000
Duration (min)
900
Fraction volume (mL)
Not specified
Fraction processing
Not specified
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: Lipid analysis
No
|
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1 - 2 of 2 |
EV-TRACK ID | EV210056 | |
---|---|---|
species | Mus musculus | |
sample type | Cell culture | |
cell type | WEHI3B | RENCA |
condition | Control condition | Control condition |
separation protocol | DG (d)(U)C | DG (d)(U)C |
Exp. nr. | 1 | 2 |
EV-METRIC % | 25 | 14 |