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You searched for: EV200189 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200189 | 1/2 | Homo sapiens | Cerebrospinal Fluid |
(d)(U)C Sonication Filtration UF |
Manek, Rachna | 2018 | 44% | |
Study summaryFull title
All authors
Rachna Manek, Ahmed Moghieb, Zhihui Yang, Dhwani Kumar, Firas Kobessiy, George Anis Sarkis, Vijaya Raghavan, Kevin K W Wang
Journal
Molecular Neurobiology
Abstract
Recently, there have been emerging interests in the area of microvesicles and exosome (MV/E) release (show more...)
EV-METRIC
44% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cerebrospinal Fluid
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Sonication Filtration Ultrafiltration Protein markers
EV: Spectrin/ UCH-L1/ Synaptophysin/ GFAP/ Alix
non-EV: None Proteomics
yes
Show all info
Study aim
Biomarker/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cerebrospinal Fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 55 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
3
Wash: time (min)
70
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
100000
Filtration steps
0.45µm > x > 0.22µm,
Ultra filtration
Cut-off size (kDa)
Not spec
Membrane type
Not specified
Other
Name other separation method
Sonication
Characterization: Protein analysis
Protein Concentration Method
No
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Spectrin/ Alix
Not detected EV-associated proteins
UCH-L1/ Synaptophysin/ GFAP
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mode
Reported size (nm)
99-104
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200189 | 2/2 | Homo sapiens | Cerebrospinal Fluid |
(d)(U)C Sonication Filtration UF |
Manek, Rachna | 2018 | 44% | |
Study summaryFull title
All authors
Rachna Manek, Ahmed Moghieb, Zhihui Yang, Dhwani Kumar, Firas Kobessiy, George Anis Sarkis, Vijaya Raghavan, Kevin K W Wang
Journal
Molecular Neurobiology
Abstract
Recently, there have been emerging interests in the area of microvesicles and exosome (MV/E) release (show more...)
EV-METRIC
44% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cerebrospinal Fluid
Sample origin
Traumatic brain injury
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Sonication Filtration Ultrafiltration Protein markers
EV: Spectrin/ UCH-L1/ Synaptophysin/ GFAP/ Alix
non-EV: None Proteomics
yes
Show all info
Study aim
Biomarker/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cerebrospinal Fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 55 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
3
Wash: time (min)
70
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
100000
Filtration steps
0.45µm > x > 0.22µm,
Ultra filtration
Cut-off size (kDa)
Not spec
Membrane type
Not specified
Other
Name other separation method
Sonication
Characterization: Protein analysis
Protein Concentration Method
No
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Spectrin/ UCH-L1/ Synaptophysin/ GFAP/ Alix
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mode
Reported size (nm)
74-98
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
|
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1 - 2 of 2 |
EV-TRACK ID | EV200189 | |
---|---|---|
species | Homo sapiens | |
sample type | Cerebrospinal Fluid | |
condition | Control condition | Traumatic brain injury |
separation protocol | dUC Sonication Filtration Ultrafiltration | dUC Sonication Filtration Ultrafiltration |
Exp. nr. | 1 | 2 |
EV-METRIC % | 44 | 44 |