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You searched for: EV200111 (EV-TRACK ID)
Showing 1 - 4 of 4
Showing 1 - 4 of 4
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200111 | 2/4 | Homo sapiens | SH-SY5Y |
(d)(U)C Filtration |
Tortolici, Flavia | 2021 | 56% | |
Study summaryFull title
All authors
Flavia Tortolici, Simone Vumbaca, Bernadette Incocciati, Renu Dayal, Katia Aquilano, Anna Giovanetti, Stefano Rufini
Journal
Cells
Abstract
Radiation therapy is one of the most effective methods of tumor eradication; however, in some forms (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Irradiated with 0.1 Gy (X-Ray)
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD81/ CD63/ Flotillin1/ CD9
non-EV: BAX Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
SH-SY5Y
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
150
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Other;absorbance of 215 nm, using known protein concentrations of commercial freeze-dried exosomes
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Flotillin1/ CD9/ CD63/ CD81
Not detected contaminants
BAX
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
Cytoflex
Hardware adjustment
The single vesicles dimensional analysis was performed by the Cytoflex high-resolution flow cytometer based on the side scatter of the violet laser (405 nm) that allows the detection of particles below 300 nm of diameter, corresponding to the size-range of exosomes.
Calibration bead size
3
Report type
Size range/distribution
Reported size (nm)
3
EV concentration
Yes
|
||||||||
EV200111 | 3/4 | Homo sapiens | SH-SY5Y |
(d)(U)C Filtration |
Tortolici, Flavia | 2021 | 56% | |
Study summaryFull title
All authors
Flavia Tortolici, Simone Vumbaca, Bernadette Incocciati, Renu Dayal, Katia Aquilano, Anna Giovanetti, Stefano Rufini
Journal
Cells
Abstract
Radiation therapy is one of the most effective methods of tumor eradication; however, in some forms (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Irradiated with 1 Gy (X-Ray)
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD81/ CD63/ Flotillin1/ CD9
non-EV: BAX Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
SH-SY5Y
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
150
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Other;absorbance of 215 nm, using known protein concentrations of commercial freeze-dried exosomes
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Flotillin1/ CD9/ CD63/ CD81
Not detected contaminants
BAX
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
Cytoflex
Hardware adjustment
The single vesicles dimensional analysis was performed by the Cytoflex high-resolution flow cytometer based on the side scatter of the violet laser (405 nm) that allows the detection of particles below 300 nm of diameter, corresponding to the size-range of exosomes.
Calibration bead size
3
Report type
Size range/distribution
Reported size (nm)
3
|
||||||||
EV200111 | 4/4 | Homo sapiens | SH-SY5Y |
(d)(U)C Filtration |
Tortolici, Flavia | 2021 | 56% | |
Study summaryFull title
All authors
Flavia Tortolici, Simone Vumbaca, Bernadette Incocciati, Renu Dayal, Katia Aquilano, Anna Giovanetti, Stefano Rufini
Journal
Cells
Abstract
Radiation therapy is one of the most effective methods of tumor eradication; however, in some forms (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Irradiated with 10 Gy (X-Ray)
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD81/ CD63/ Flotillin1/ CD9
non-EV: BAX Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
SH-SY5Y
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
150
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Other;absorbance of 215 nm, using known protein concentrations of commercial freeze-dried exosomes
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Flotillin1/ CD9/ CD63/ CD81
Not detected contaminants
BAX
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
Cytoflex
Hardware adjustment
The single vesicles dimensional analysis was performed by the Cytoflex high-resolution flow cytometer based on the side scatter of the violet laser (405 nm) that allows the detection of particles below 300 nm of diameter, corresponding to the size-range of exosomes.
Calibration bead size
3
Report type
Size range/distribution
Reported size (nm)
3
EV concentration
Yes
|
||||||||
EV200111 | 1/4 | Homo sapiens | SH-SY5Y |
(d)(U)C Filtration |
Tortolici, Flavia | 2021 | 43% | |
Study summaryFull title
All authors
Flavia Tortolici, Simone Vumbaca, Bernadette Incocciati, Renu Dayal, Katia Aquilano, Anna Giovanetti, Stefano Rufini
Journal
Cells
Abstract
Radiation therapy is one of the most effective methods of tumor eradication; however, in some forms (show more...)
EV-METRIC
43% (82nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD81/ CD63/ Flotillin1/ CD9
non-EV: BAX Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
SH-SY5Y
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
150
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Other;absorbance of 215 nm, using known protein concentrations of commercial freeze-dried exosomes
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Flotillin1/ CD9/ CD63/ CD81
Not detected contaminants
BAX
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
Cytoflex
Hardware adjustment
The single vesicles dimensional analysis was performed by the Cytoflex high-resolution flow cytometer based on the side scatter of the violet laser (405 nm) that allows the detection of particles below 300 nm of diameter, corresponding to the size-range of exosomes.
Calibration bead size
3
EV concentration
Yes
|
||||||||
1 - 4 of 4 |
EV-TRACK ID | EV200111 | |||
---|---|---|---|---|
species | Homo sapiens | |||
sample type | Cell culture | |||
cell type | SH-SY5Y | |||
condition | Irradiated with 0.1 Gy (X-Ray) | Irradiated with 1 Gy (X-Ray) | Irradiated with 10 Gy (X-Ray) | Control condition |
separation protocol | dUC Filtration | dUC Filtration | dUC Filtration | dUC Filtration |
Exp. nr. | 2 | 3 | 4 | 1 |
EV-METRIC % | 56 | 56 | 56 | 43 |