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You searched for: EV200076 (EV-TRACK ID)
Showing 1 - 5 of 5
Showing 1 - 5 of 5
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200076 | 1/5 | microalgae | Dinoflagellate | (d)(U)C | Picciotto, Sabrina | 2021 | 67% | |
Study summaryFull title
All authors
Sabrina Picciotto, Maria E. Barone, David Fierli, Anita Aranyos, Giorgia Adamo, Darja Božič, Daniele P. Romancino, Christopher Stanly, Rachel Parkes, Svenja Morsbach, Samuele Raccosta, Carolina Paganini, Antonella Cusimano, Vincenzo Martorana, Rosina Noto, Rita Carrotta, Fabio Librizzi, Umberto Capasso Palmiero, Pamela Santonicola, Ales Iglič, Meiyu Gai, Laura Corcuera, Annamaria Kisslinger, Elia Di Schiavi, Katharina Landfester, Giovanna L. Liguori, Veronika Kralj-Iglič, Paolo Arosio, Gabriella Pocsfalvi, Mauro Manno, Nicolas Touzet, Antonella Bongiovanni
Journal
Biomaterials science
Abstract
Safe, efficient and specific nano-delivery systems are essential for current and emerging therapeuti (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Alix/ HSP70/ beta-actin/ enolase
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/novel EV type
Sample
Species
microalgae
Sample Type
Cell culture supernatant
EV-producing cells
Dinoflagellate
EV-harvesting Medium
Serum free medium
Cell viability (%)
90
Cell count
1 mg dry weight biomass/ml
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 28
Pelleting: speed (g)
118000
Wash: volume per pellet (ml)
32
Wash: time (min)
70
Wash: Rotor Type
SW 28
Wash: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
beta-actin/ enolase/ HSP70/ Alix
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
92
NTA
Report type
Size range/distribution
Reported size (nm)
125
EV concentration
Yes
Particle yield
number of particles per mg dry weight microalgal mass 6.00E+09
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
|
||||||||
EV200076 | 2/5 | microalgae | Diatom | (d)(U)C | Picciotto, Sabrina | 2021 | 67% | |
Study summaryFull title
All authors
Sabrina Picciotto, Maria E. Barone, David Fierli, Anita Aranyos, Giorgia Adamo, Darja Božič, Daniele P. Romancino, Christopher Stanly, Rachel Parkes, Svenja Morsbach, Samuele Raccosta, Carolina Paganini, Antonella Cusimano, Vincenzo Martorana, Rosina Noto, Rita Carrotta, Fabio Librizzi, Umberto Capasso Palmiero, Pamela Santonicola, Ales Iglič, Meiyu Gai, Laura Corcuera, Annamaria Kisslinger, Elia Di Schiavi, Katharina Landfester, Giovanna L. Liguori, Veronika Kralj-Iglič, Paolo Arosio, Gabriella Pocsfalvi, Mauro Manno, Nicolas Touzet, Antonella Bongiovanni
Journal
Biomaterials science
Abstract
Safe, efficient and specific nano-delivery systems are essential for current and emerging therapeuti (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Alix/ HSP70/ enolase
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/novel EV type
Sample
Species
microalgae
Sample Type
Cell culture supernatant
EV-producing cells
Diatom
EV-harvesting Medium
Serum free medium
Cell viability (%)
90
Cell count
1 mg dry weight biomass/ml
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 28
Pelleting: speed (g)
118000
Wash: volume per pellet (ml)
32
Wash: time (min)
70
Wash: Rotor Type
SW 28
Wash: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
enolase/ HSP70/ Alix
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
135
NTA
Report type
Size range/distribution
Reported size (nm)
90
EV concentration
Yes
Particle yield
number of particles per mg dry weight microalgal mass 2.40E+08
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
|
||||||||
EV200076 | 3/5 | microalgae | Glaucophyte | (d)(U)C | Picciotto, Sabrina | 2021 | 67% | |
Study summaryFull title
All authors
Sabrina Picciotto, Maria E. Barone, David Fierli, Anita Aranyos, Giorgia Adamo, Darja Božič, Daniele P. Romancino, Christopher Stanly, Rachel Parkes, Svenja Morsbach, Samuele Raccosta, Carolina Paganini, Antonella Cusimano, Vincenzo Martorana, Rosina Noto, Rita Carrotta, Fabio Librizzi, Umberto Capasso Palmiero, Pamela Santonicola, Ales Iglič, Meiyu Gai, Laura Corcuera, Annamaria Kisslinger, Elia Di Schiavi, Katharina Landfester, Giovanna L. Liguori, Veronika Kralj-Iglič, Paolo Arosio, Gabriella Pocsfalvi, Mauro Manno, Nicolas Touzet, Antonella Bongiovanni
Journal
Biomaterials science
Abstract
Safe, efficient and specific nano-delivery systems are essential for current and emerging therapeuti (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Alix/ HSP70/ beta-actin/ enolase
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/novel EV type
Sample
Species
microalgae
Sample Type
Cell culture supernatant
EV-producing cells
Glaucophyte
EV-harvesting Medium
Serum free medium
Cell viability (%)
90
Cell count
1 mg dry weight biomass/ml
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 28
Pelleting: speed (g)
118000
Wash: volume per pellet (ml)
32
Wash: time (min)
70
Wash: Rotor Type
SW 28
Wash: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ HSP70/ beta-actin/ enolase
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
125
NTA
Report type
Size range/distribution
Reported size (nm)
122
EV concentration
Yes
Particle yield
number of particles per mg dry weight microalgal mass 2.00E+09
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
|
||||||||
EV200076 | 4/5 | microalgae | Haptophyte | (d)(U)C | Picciotto, Sabrina | 2021 | 67% | |
Study summaryFull title
All authors
Sabrina Picciotto, Maria E. Barone, David Fierli, Anita Aranyos, Giorgia Adamo, Darja Božič, Daniele P. Romancino, Christopher Stanly, Rachel Parkes, Svenja Morsbach, Samuele Raccosta, Carolina Paganini, Antonella Cusimano, Vincenzo Martorana, Rosina Noto, Rita Carrotta, Fabio Librizzi, Umberto Capasso Palmiero, Pamela Santonicola, Ales Iglič, Meiyu Gai, Laura Corcuera, Annamaria Kisslinger, Elia Di Schiavi, Katharina Landfester, Giovanna L. Liguori, Veronika Kralj-Iglič, Paolo Arosio, Gabriella Pocsfalvi, Mauro Manno, Nicolas Touzet, Antonella Bongiovanni
Journal
Biomaterials science
Abstract
Safe, efficient and specific nano-delivery systems are essential for current and emerging therapeuti (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Alix/ HSP70/ enolase
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/novel EV type
Sample
Species
microalgae
Sample Type
Cell culture supernatant
EV-producing cells
Haptophyte
EV-harvesting Medium
Serum free medium
Cell viability (%)
90
Cell count
1 mg dry weight biomass/ml
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 28
Pelleting: speed (g)
118000
Wash: volume per pellet (ml)
32
Wash: time (min)
70
Wash: Rotor Type
SW 28
Wash: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ enolase
Not detected EV-associated proteins
HSP70
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
87
NTA
Report type
Size range/distribution
Reported size (nm)
165
EV concentration
Yes
Particle yield
number of particles per mg dry weight microalgal mass 1.30E+08
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
|
||||||||
EV200076 | 5/5 | microalgae | Chlorophyte | (d)(U)C | Picciotto, Sabrina | 2021 | 67% | |
Study summaryFull title
All authors
Sabrina Picciotto, Maria E. Barone, David Fierli, Anita Aranyos, Giorgia Adamo, Darja Božič, Daniele P. Romancino, Christopher Stanly, Rachel Parkes, Svenja Morsbach, Samuele Raccosta, Carolina Paganini, Antonella Cusimano, Vincenzo Martorana, Rosina Noto, Rita Carrotta, Fabio Librizzi, Umberto Capasso Palmiero, Pamela Santonicola, Ales Iglič, Meiyu Gai, Laura Corcuera, Annamaria Kisslinger, Elia Di Schiavi, Katharina Landfester, Giovanna L. Liguori, Veronika Kralj-Iglič, Paolo Arosio, Gabriella Pocsfalvi, Mauro Manno, Nicolas Touzet, Antonella Bongiovanni
Journal
Biomaterials science
Abstract
Safe, efficient and specific nano-delivery systems are essential for current and emerging therapeuti (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Alix/ beta-actin/ enolase
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/novel EV type
Sample
Species
microalgae
Sample Type
Cell culture supernatant
EV-producing cells
Chlorophyte
EV-harvesting Medium
Serum free medium
Cell viability (%)
90
Cell count
1 mg dry weight biomass/ml
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 28
Pelleting: speed (g)
118000
Wash: volume per pellet (ml)
32
Wash: time (min)
70
Wash: Rotor Type
SW 28
Wash: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
beta-actin/ enolase/ Alix
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
75
NTA
Report type
Size range/distribution
Reported size (nm)
137
EV concentration
Yes
Particle yield
number of particles per mg dry weight microalgal mass 2.60E+08
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
|
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1 - 5 of 5 |
EV-TRACK ID | EV200076 | ||||
---|---|---|---|---|---|
species | microalgae | ||||
sample type | Cell culture | ||||
cell type | Dinoflagellate | Diatom | Glaucophyte | Haptophyte | Chlorophyte |
condition | Control condition | Control condition | Control condition | Control condition | Control condition |
separation protocol | (d)(U)C | (d)(U)C | (d)(U)C | (d)(U)C | (d)(U)C |
Exp. nr. | 1 | 2 | 3 | 4 | 5 |
EV-METRIC % | 67 | 67 | 67 | 67 | 67 |