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You searched for: EV200054 (EV-TRACK ID)
Showing 1 - 6 of 6
Showing 1 - 6 of 6
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200054 | 1/7 | Homo sapiens | Brain tissue |
(d)(U)C Filtration DC |
Huang, Yiyao | 2020 | 67% | |
Study summaryFull title
All authors
Yiyao Huang, Lesley Cheng, Andrey Turchinovich, Vasiliki Mahairaki, Juan C Troncoso, Olga Pletniková, Norman J Haughey, Laura J Vella, Andrew F Hill, Lei Zheng, Kenneth W Witwer
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) are involved in a wide range of physiological and pathological processe (show more...)
EV-METRIC
67% (81st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Brain tissue
Sample origin
Control condition
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration DC Protein markers
EV: CD63/ CD81/ CD9/ TSG101
non-EV: GM130/ Calnexin Proteomics
no
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV- related methods
Sample
Species
Homo sapiens
Sample Type
Brain tissue
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Pelleting: rotor type
AH-650
Pelleting: speed (g)
10000
Filtration steps
0.22µm or 0.2µm
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ CD81
Not detected EV-associated proteins
CD9/ TSG101
Not detected contaminants
GM130/ Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200054 | 2/7 | Homo sapiens | Brain tissue |
(d)(U)C Filtration qEV |
Huang, Yiyao | 2020 | 67% | |
Study summaryFull title
All authors
Yiyao Huang, Lesley Cheng, Andrey Turchinovich, Vasiliki Mahairaki, Juan C Troncoso, Olga Pletniková, Norman J Haughey, Laura J Vella, Andrew F Hill, Lei Zheng, Kenneth W Witwer
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) are involved in a wide range of physiological and pathological processe (show more...)
EV-METRIC
67% (81st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Brain tissue
Sample origin
Control condition
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Commercial method Protein markers
EV: CD63/ CD81/ Syntenin
non-EV: GM130/ Calnexin Proteomics
yes
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV- related methods
Sample
Species
Homo sapiens
Sample Type
Brain tissue
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Pelleting: rotor type
AH-650
Pelleting: speed (g)
10000
Filtration steps
0.22µm or 0.2µm
Commercial kit
qEV
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ CD81
Not detected EV-associated proteins
Syntenin
Not detected contaminants
GM130/ Calnexin
Proteomics database
No
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Yes
Reported size (nm)
40-145
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200054 | 3/7 | Homo sapiens | Brain tissue |
(d)(U)C Filtration |
Huang, Yiyao | 2020 | 67% | |
Study summaryFull title
All authors
Yiyao Huang, Lesley Cheng, Andrey Turchinovich, Vasiliki Mahairaki, Juan C Troncoso, Olga Pletniková, Norman J Haughey, Laura J Vella, Andrew F Hill, Lei Zheng, Kenneth W Witwer
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) are involved in a wide range of physiological and pathological processe (show more...)
EV-METRIC
67% (81st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Brain tissue
Sample origin
Control condition
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: CD63/ CD81/ Syntenin
non-EV: GM130/ Calnexin Proteomics
no
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV- related methods
Sample
Species
Homo sapiens
Sample Type
Brain tissue
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Pelleting: rotor type
AH-650
Pelleting: speed (g)
10000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ CD81/ Syntenin
Detected contaminants
Calnexin
Not detected contaminants
GM130
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200054 | 4/7 | Mus musculus | Brain tissue |
(d)(U)C Filtration DC |
Huang, Yiyao | 2020 | 67% | |
Study summaryFull title
All authors
Yiyao Huang, Lesley Cheng, Andrey Turchinovich, Vasiliki Mahairaki, Juan C Troncoso, Olga Pletniková, Norman J Haughey, Laura J Vella, Andrew F Hill, Lei Zheng, Kenneth W Witwer
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) are involved in a wide range of physiological and pathological processe (show more...)
EV-METRIC
67% (81st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Brain tissue
Sample origin
Control condition
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration DC Protein markers
EV: CD9/ TSG101/ CD81/ Syntenin
non-EV: GM130/ Calnexin Proteomics
no
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV- related methods
Sample
Species
Mus musculus
Sample Type
Brain tissue
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Pelleting: rotor type
AH-650
Pelleting: speed (g)
10000
Filtration steps
0.22µm or 0.2µm
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ TSG101
Not detected EV-associated proteins
CD81/ Syntenin
Detected contaminants
GM130/ Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200054 | 5/7 | Mus musculus | Brain tissue |
(d)(U)C Filtration qEV |
Huang, Yiyao | 2020 | 56% | |
Study summaryFull title
All authors
Yiyao Huang, Lesley Cheng, Andrey Turchinovich, Vasiliki Mahairaki, Juan C Troncoso, Olga Pletniková, Norman J Haughey, Laura J Vella, Andrew F Hill, Lei Zheng, Kenneth W Witwer
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) are involved in a wide range of physiological and pathological processe (show more...)
EV-METRIC
56% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Brain tissue
Sample origin
Control condition
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Commercial method Protein markers
EV: Rab27a/ TSG101
non-EV: GM130/ Calnexin Proteomics
no
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV- related methods
Sample
Species
Mus musculus
Sample Type
Brain tissue
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Pelleting: rotor type
AH-650
Pelleting: speed (g)
10000
Filtration steps
0.22µm or 0.2µm
Commercial kit
qEV
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Rab27a
Not detected EV-associated proteins
TSG101
Detected contaminants
GM130/ Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200054 | 6/7 | Mus musculus | Brain tissue |
(d)(U)C Filtration qEV UF |
Huang, Yiyao | 2020 | 44% | |
Study summaryFull title
All authors
Yiyao Huang, Lesley Cheng, Andrey Turchinovich, Vasiliki Mahairaki, Juan C Troncoso, Olga Pletniková, Norman J Haughey, Laura J Vella, Andrew F Hill, Lei Zheng, Kenneth W Witwer
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) are involved in a wide range of physiological and pathological processe (show more...)
EV-METRIC
44% (51st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Brain tissue
Sample origin
Control condition
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Commercial method UF Protein markers
EV: CD9/ TSG101
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV- related methods
Sample
Species
Mus musculus
Sample Type
Brain tissue
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Pelleting: rotor type
AH-650
Pelleting: speed (g)
10000
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Commercial kit
qEV
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ TSG101
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
1 - 6 of 6 |
EV-TRACK ID | EV200054 | |||||
---|---|---|---|---|---|---|
species | Homo sapiens | Homo sapiens | Homo sapiens | Mus musculus | Mus musculus | Mus musculus |
sample type | Brain tissue | Brain tissue | Brain tissue | Brain tissue | Brain tissue | Brain tissue |
condition | Control condition | Control condition | Control condition | Control condition | Control condition | Control condition |
separation protocol | (d)(U)C Filtration DC | (d)(U)C Filtration qEV | (d)(U)C Filtration | (d)(U)C Filtration DC | (d)(U)C Filtration qEV | (d)(U)C Filtration qEV UF |
Exp. nr. | 1 | 2 | 3 | 4 | 5 | 6 |
EV-METRIC % | 67 | 67 | 67 | 67 | 56 | 44 |