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You searched for: EV200033 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200033 | 2/2 | Indoor dust | Indoor dust |
DG (d)(U)C Dissolving indoor dust in PBS Filtration |
Dinh, Nhung Thi Hong | 2020 | 71% | |
Study summaryFull title
All authors
Nhung Thi Hong Dinh, Jaewook Lee, Jaemin Lee, Sang Soo Kim, Gyeongyun Go, Seoyoon Bae, Ye In Jun, Yae Jin Yoon, Tae-Young Roh, Yong Song Gho
Journal
J Extracell Vesicles
Abstract
Indoor pollutants are important problems to public health. Among indoor pollutants, indoor dust cont (show more...)
EV-METRIC
71% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Indoor dust
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C Dissolving indoor dust in PBS Filtration Protein markers
EV: None
non-EV: None Proteomics
no
EV density (g/ml)
N/A
Show all info
Study aim
Function
Sample
Species
Indoor dust
Sample Type
Indoor dust
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
180
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
150000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
3
Lowest density fraction
10%
Highest density fraction
50%
Total gradient volume, incl. sample (mL)
5.25
Sample volume (mL)
2.5
Orientation
Bottom-up
Rotor type
SW 55 Ti
Speed (g)
200000
Duration (min)
120
Fraction volume (mL)
0.5
Fraction processing
None
Filtration steps
0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Other
Name other separation method
Dissolving indoor dust in PBS
Characterization: Protein analysis
None
Protein Concentration Method
Bradford
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
129.6
NTA
Report type
Not Reported
EV concentration
Yes
Particle yield
as number of particle per gram of starting sample;Yes, other: 1.10E+11
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
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EV200033 | 1/2 | Pseudomonas aeruginosa | PAO1 |
DG (d)(U)C UF Filtration |
Dinh, Nhung Thi Hong | 2020 | 57% | |
Study summaryFull title
All authors
Nhung Thi Hong Dinh, Jaewook Lee, Jaemin Lee, Sang Soo Kim, Gyeongyun Go, Seoyoon Bae, Ye In Jun, Yae Jin Yoon, Tae-Young Roh, Yong Song Gho
Journal
J Extracell Vesicles
Abstract
Indoor pollutants are important problems to public health. Among indoor pollutants, indoor dust cont (show more...)
EV-METRIC
57% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C UF Filtration Protein markers
EV: None
non-EV: None Proteomics
no
EV density (g/ml)
N/A
Show all info
Study aim
Function
Sample
Species
Pseudomonas aeruginosa
Sample Type
Cell culture supernatant
EV-producing cells
PAO1
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
180
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
150000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
3
Lowest density fraction
10%
Highest density fraction
50%
Total gradient volume, incl. sample (mL)
5.25
Sample volume (mL)
2.5
Orientation
Bottom-up
Rotor type
SW 55 Ti
Speed (g)
200000
Duration (min)
120
Fraction volume (mL)
0.5
Fraction processing
None
Filtration steps
0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polysulfone;Other
Characterization: Protein analysis
None
Protein Concentration Method
Bradford
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
81.9
EM
EM-type
Transmission-EM
Image type
Wide-field
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1 - 2 of 2 |
EV-TRACK ID | EV200033 | |
---|---|---|
species | Indoor dust | Pseudomonas aeruginosa |
sample type | Indoor dust | Cell culture |
cell type | NA | PAO1 |
medium | NA | Serum free medium |
condition | Control condition | Control condition |
separation protocol | DG (d)(U)C Dissolving indoor dust in PBS Filtration | DG (d)(U)C UF Filtration |
Exp. nr. | 2 | 1 |
EV-METRIC % | 71 | 57 |