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You searched for: EV190107 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV190107 | 2/3 | Homo sapiens | Jurkat |
DG (d)(U)C |
Martin-Jaular, Lorena | 2021 | 67% | |
Study summaryFull title
All authors
Lorena Martin-Jaular, Nathalie Nevo, Julia P Schessner, Mercedes Tkach, Mabel Jouve, Florent Dingli, Damarys Loew, Kenneth W Witwer, Matias Ostrowski, Georg H H Borner, Clotilde Théry
Journal
EMBO J
Abstract
Cells release diverse types of extracellular vesicles (EVs), which transfer complex signals to surro (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: "cd45/ AChE/ CD63/ CD9/ syntenin-1"
non-EV: Proteomics
no
EV density (g/ml)
1.001-1.097
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Jurkat
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Cell viability (%)
87
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
106750
Wash: volume per pellet (ml)
37
Wash: time (min)
90
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
106750
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
6%
Highest density fraction
18%
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
1
Orientation
Top-down
Rotor type
SW 41 Ti
Speed (g)
200000
Duration (min)
60
Fraction volume (mL)
1 or 2
Fraction processing
Centrifugation
Pelleting: volume per fraction
37
Pelleting: duration (min)
90
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
106750
EV-subtype
Distinction between multiple subtypes
Used subtypes
Characterization: Protein analysis
Protein Concentration Method
"Other;Gel stain free assay"
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
"CD9/ CD63/ syntenin-1/ cd45"
Not detected EV-associated proteins
AChE
Characterization: Lipid analysis
No
|
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EV190107 | 1/3 | Homo sapiens | Jurkat | (d)(U)C | Martin-Jaular, Lorena | 2021 | 56% | |
Study summaryFull title
All authors
Lorena Martin-Jaular, Nathalie Nevo, Julia P Schessner, Mercedes Tkach, Mabel Jouve, Florent Dingli, Damarys Loew, Kenneth W Witwer, Matias Ostrowski, Georg H H Borner, Clotilde Théry
Journal
EMBO J
Abstract
Cells release diverse types of extracellular vesicles (EVs), which transfer complex signals to surro (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: "Cd45/ actin/ CD63/ CD9/ Syntenin-1"
non-EV: "GP96/ AChE" Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Jurkat
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Cell viability (%)
87
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
106750
Wash: volume per pellet (ml)
37
Wash: time (min)
90
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
106750
EV-subtype
Distinction between multiple subtypes
Used subtypes
Characterization: Protein analysis
Protein Concentration Method
"Other;Gel stain free assay"
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
"CD9/ CD63/ Syntenin-1/ actin/ Cd45"
Not detected contaminants
"AChE/ GP96"
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
30-200
|
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EV190107 | 3/3 | Homo sapiens | Jurkat |
IAF IAF qEV |
Martin-Jaular, Lorena | 2021 | 38% | |
Study summaryFull title
All authors
Lorena Martin-Jaular, Nathalie Nevo, Julia P Schessner, Mercedes Tkach, Mabel Jouve, Florent Dingli, Damarys Loew, Kenneth W Witwer, Matias Ostrowski, Georg H H Borner, Clotilde Théry
Journal
EMBO J
Abstract
Cells release diverse types of extracellular vesicles (EVs), which transfer complex signals to surro (show more...)
EV-METRIC
38% (80th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
IAF
Immunoaffinity capture (non-commercial) qEV Protein markers
EV: "MOV10/ CD63/ SPN/ SERINC3"
non-EV: Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Jurkat
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Cell viability (%)
87
Separation Method
Commercial kit
qEV
Immunoaffinity capture
Selected surface protein(s)
SPN
Other
Name other separation method
qEV
EV-subtype
Distinction between multiple subtypes
Used subtypes
Characterization: Protein analysis
Protein Concentration Method
"Other;Gel stain free assay"
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
"CD63/ SERINC3/ SPN/ MOV10"
Other 1
Detected EV-associated proteins
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Not Reported
EV concentration
Yes
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1 - 3 of 3 |
EV-TRACK ID | EV190107 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Cell culture | ||
cell type | Jurkat | ||
condition | Control condition | ||
separation protocol | DG (d)(U)C | (d)(U)C | IAF IAF capture (non-commercial) qEV |
Exp. nr. | 2 | 1 | 3 |
EV-METRIC % | 67 | 56 | 38 |