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You searched for: EV190100 (EV-TRACK ID)
Showing 1 - 10 of 10
Showing 1 - 10 of 10
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV190100 | 1/10 | Homo sapiens | CNE1 | ExoQuick | Chaoliang Liao | 2020 | 50% | |
Study summaryFull title
All authors
Chaoliang Liao, Qin Zhou, Zhibao Zhang, Xia Wu, Zhuan Zhou, Bo Li, Jinwu Peng, Liangfang Shen, Dan Li, Xiangjian Luo, Lifang Yang
Journal
J Pharm Sci
Abstract
Increasing evidence indicates that extracellular vesicles (EVs) play an important role in cancer cel (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: HSP70/ CD63
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
CNE1
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ HSP70
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
21.04-255.6
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
80-100
|
||||||||
EV190100 | 2/10 | Homo sapiens | CNE1-LMP1 | ExoQuick | Chaoliang Liao | 2020 | 50% | |
Study summaryFull title
All authors
Chaoliang Liao, Qin Zhou, Zhibao Zhang, Xia Wu, Zhuan Zhou, Bo Li, Jinwu Peng, Liangfang Shen, Dan Li, Xiangjian Luo, Lifang Yang
Journal
J Pharm Sci
Abstract
Increasing evidence indicates that extracellular vesicles (EVs) play an important role in cancer cel (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: HSP70/ CD63
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
CNE1-LMP1
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ HSP70
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
80-100
|
||||||||
EV190100 | 6/10 | Homo sapiens | HKI | ExoQuick | Chaoliang Liao | 2020 | 50% | |
Study summaryFull title
All authors
Chaoliang Liao, Qin Zhou, Zhibao Zhang, Xia Wu, Zhuan Zhou, Bo Li, Jinwu Peng, Liangfang Shen, Dan Li, Xiangjian Luo, Lifang Yang
Journal
J Pharm Sci
Abstract
Increasing evidence indicates that extracellular vesicles (EVs) play an important role in cancer cel (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: HSP70/ CD63
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HKI
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ HSP70
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
24.3-199.2
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
80-100
|
||||||||
EV190100 | 8/10 | Homo sapiens | C666-1 | ExoQuick | Chaoliang Liao | 2020 | 50% | |
Study summaryFull title
All authors
Chaoliang Liao, Qin Zhou, Zhibao Zhang, Xia Wu, Zhuan Zhou, Bo Li, Jinwu Peng, Liangfang Shen, Dan Li, Xiangjian Luo, Lifang Yang
Journal
J Pharm Sci
Abstract
Increasing evidence indicates that extracellular vesicles (EVs) play an important role in cancer cel (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: HSP70/ CD63
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
C666-1
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ HSP70
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
13.54-225.9
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
80-100
|
||||||||
EV190100 | 3/10 | Homo sapiens | CNE1-LMP1 | ExoQuick | Chaoliang Liao | 2020 | 0% | |
Study summaryFull title
All authors
Chaoliang Liao, Qin Zhou, Zhibao Zhang, Xia Wu, Zhuan Zhou, Bo Li, Jinwu Peng, Liangfang Shen, Dan Li, Xiangjian Luo, Lifang Yang
Journal
J Pharm Sci
Abstract
Increasing evidence indicates that extracellular vesicles (EVs) play an important role in cancer cel (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
transfected siLMP1
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
CNE1-LMP1
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
|
||||||||
EV190100 | 4/10 | Homo sapiens | CNE1-LMP1 | ExoQuick | Chaoliang Liao | 2020 | 0% | |
Study summaryFull title
All authors
Chaoliang Liao, Qin Zhou, Zhibao Zhang, Xia Wu, Zhuan Zhou, Bo Li, Jinwu Peng, Liangfang Shen, Dan Li, Xiangjian Luo, Lifang Yang
Journal
J Pharm Sci
Abstract
Increasing evidence indicates that extracellular vesicles (EVs) play an important role in cancer cel (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
transfected siSDC2
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
CNE1-LMP1
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
|
||||||||
EV190100 | 5/10 | Homo sapiens | CNE1-LMP1 | ExoQuick | Chaoliang Liao | 2020 | 0% | |
Study summaryFull title
All authors
Chaoliang Liao, Qin Zhou, Zhibao Zhang, Xia Wu, Zhuan Zhou, Bo Li, Jinwu Peng, Liangfang Shen, Dan Li, Xiangjian Luo, Lifang Yang
Journal
J Pharm Sci
Abstract
Increasing evidence indicates that extracellular vesicles (EVs) play an important role in cancer cel (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
transfected siSYTL4
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
CNE1-LMP1
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
|
||||||||
EV190100 | 7/10 | Homo sapiens | HKI | ExoQuick | Chaoliang Liao | 2020 | 0% | |
Study summaryFull title
All authors
Chaoliang Liao, Qin Zhou, Zhibao Zhang, Xia Wu, Zhuan Zhou, Bo Li, Jinwu Peng, Liangfang Shen, Dan Li, Xiangjian Luo, Lifang Yang
Journal
J Pharm Sci
Abstract
Increasing evidence indicates that extracellular vesicles (EVs) play an important role in cancer cel (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
transfected LMP1
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HKI
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
|
||||||||
EV190100 | 9/10 | Homo sapiens | C666-1 | ExoQuick | Chaoliang Liao | 2020 | 0% | |
Study summaryFull title
All authors
Chaoliang Liao, Qin Zhou, Zhibao Zhang, Xia Wu, Zhuan Zhou, Bo Li, Jinwu Peng, Liangfang Shen, Dan Li, Xiangjian Luo, Lifang Yang
Journal
J Pharm Sci
Abstract
Increasing evidence indicates that extracellular vesicles (EVs) play an important role in cancer cel (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
transfected siLMP1
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
C666-1
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
|
||||||||
EV190100 | 10/10 | Homo sapiens | C666-1 | ExoQuick | Chaoliang Liao | 2020 | 0% | |
Study summaryFull title
All authors
Chaoliang Liao, Qin Zhou, Zhibao Zhang, Xia Wu, Zhuan Zhou, Bo Li, Jinwu Peng, Liangfang Shen, Dan Li, Xiangjian Luo, Lifang Yang
Journal
J Pharm Sci
Abstract
Increasing evidence indicates that extracellular vesicles (EVs) play an important role in cancer cel (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
transfected siSDC2
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
C666-1
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
|
||||||||
1 - 10 of 10 |
EV-TRACK ID | EV190100 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
species | Homo sapiens | |||||||||
sample type | Cell culture | |||||||||
cell type | CNE1 | CNE1-LMP1 | HKI | C666-1 | CNE1-LMP1 | CNE1-LMP1 | CNE1-LMP1 | HKI | C666-1 | C666-1 |
condition | Control condition | Control condition | Control condition | Control condition | transfected siLMP1 | transfected siSDC2 | transfected siSYTL4 | transfected LMP1 | transfected siLMP1 | transfected siSDC2 |
separation protocol | ExoQuick | ExoQuick | ExoQuick | ExoQuick | ExoQuick | ExoQuick | ExoQuick | ExoQuick | ExoQuick | ExoQuick |
Exp. nr. | 1 | 2 | 6 | 8 | 3 | 4 | 5 | 7 | 9 | 10 |
EV-METRIC % | 50 | 50 | 50 | 50 | 0 | 0 | 0 | 0 | 0 | 0 |