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You searched for: EV190046 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV190046 | 1/2 | Homo sapiens | THP-1 | (d)(U)C | Deville, Sarah | 2021 | 56% | |
Study summaryFull title
All authors
Sarah Deville, Pascale Berckmans, Rebekka Van Hoof, Ivo Lambrichts, Anna Salvati, Inge Nelissen
Journal
PLoS One
Abstract
Extracellular vesicles (EVs) are of interest for a wide variety of biomedical applications. A major (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
LPS-stimulated
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: / TSG101/ Flotillin1/ CD9
non-EV: Cytochrome C Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
THP-1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ CD9/ TSG101
Not detected contaminants
Cytochrome C
Flow cytometry
Type of Flow cytometry
Calibration bead size
Antibody details provided?
No
Detected EV-associated proteins
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
182
EV concentration
Yes
Particle analysis: flow cytometry
Flow cytometer type
BD Influx
Hardware adjustment
A BD Influx flow cytometer equipped with a high power 488-nm laser (200 mW) and a small-particle detector for high sensitivity forward scatter detection was used for analysis. The device utilizes a highly sensitive fluorescence trigger to measure the EVs.
Calibration bead size
0.1
EV concentration
Yes
|
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EV190046 | 2/2 | Homo sapiens | THP-1 | exoEasy (Qiagen) | Deville, Sarah | 2021 | 50% | |
Study summaryFull title
All authors
Sarah Deville, Pascale Berckmans, Rebekka Van Hoof, Ivo Lambrichts, Anna Salvati, Inge Nelissen
Journal
PLoS One
Abstract
Extracellular vesicles (EVs) are of interest for a wide variety of biomedical applications. A major (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
LPS-stimulated
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
exoEasy (Qiagen)
Protein markers
EV: / TSG101/ Flotillin1/ CD9
non-EV: Cytochrome C/ Cytochrome c Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
THP-1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
Commercial kit
Other;exoEasy (Qiagen)
Other
Name other separation method
exoEasy (Qiagen)
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ CD9/ TSG101
Not detected contaminants
Cytochrome C
Flow cytometry
Type of Flow cytometry
Calibration bead size
Antibody details provided?
No
Detected EV-associated proteins
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
210
EV concentration
Yes
Particle analysis: flow cytometry
Flow cytometer type
BD Influx
Hardware adjustment
A BD Influx flow cytometer equipped with a high power 488-nm laser (200 mW) and a small-particle detector for high sensitivity forward scatter detection was used for analysis. The device utilizes a highly sensitive fluorescence trigger to measure the EVs.
Calibration bead size
0.1
Report type
Not Reported
EV concentration
Yes
|
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1 - 2 of 2 |
EV-TRACK ID | EV190046 | |
---|---|---|
species | Homo sapiens | |
sample type | Cell culture | |
cell type | THP-1 | |
condition | LPS-stimulated | |
separation protocol | (d)(U)C | exoEasy (Qiagen) |
Exp. nr. | 1 | 2 |
EV-METRIC % | 56 | 50 |