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You searched for: EV190019 (EV-TRACK ID)
Showing 1 - 4 of 4
Showing 1 - 4 of 4
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV190019 | 1/4 | Mus musculus | C2C12 |
DG (d)(U)C |
Leermakers PA | 2019 | 44% | |
Study summaryFull title
All authors
Leermakers PA, Remels AHV, Zonneveld MI, Rouschop KMA, Schols AMWJ, Gosker HR.
Journal
FASEB J
Abstract
Iron homeostasis is essential for mitochondrial function, and iron deficiency has been associated wi (show more...)
EV-METRIC
44% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: HSP70/ Flotillin1
non-EV: Proteomics
no
EV density (g/ml)
1.12-1.19 g/ml
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)/Mechanism of uptake/transfer
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
C2C12
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Density gradient
Type
Continuous
Lowest density fraction
0.4M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
12.4
Sample volume (mL)
0.2
Orientation
Bottom-up
Rotor type
SW 41 Ti
Speed (g)
188000
Duration (min)
16
Fraction volume (mL)
1 ml
Fraction processing
Centrifugation
Pelleting: volume per fraction
6 ml
Pelleting: duration (min)
65
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
EV-subtype
Distinction between multiple subtypes
sedimentation speed;Other
Used subtypes
16,000g
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ HSP70
Characterization: Lipid analysis
No
EM
EM-type
Cryo-EM
Image type
Wide-field
|
||||||||
EV190019 | 2/4 | Mus musculus | C2C12 |
DG (d)(U)C |
Leermakers PA | 2019 | 44% | |
Study summaryFull title
All authors
Leermakers PA, Remels AHV, Zonneveld MI, Rouschop KMA, Schols AMWJ, Gosker HR.
Journal
FASEB J
Abstract
Iron homeostasis is essential for mitochondrial function, and iron deficiency has been associated wi (show more...)
EV-METRIC
44% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: HSP70/ Flotillin1
non-EV: Proteomics
no
EV density (g/ml)
1.12-1.19 g/ml
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)/Mechanism of uptake/transfer
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
C2C12
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Density gradient
Type
Continuous
Lowest density fraction
0.4M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
12.4
Sample volume (mL)
0.2
Orientation
Bottom-up
Rotor type
SW 41 Ti
Speed (g)
188000
Duration (min)
16
Fraction volume (mL)
1 ml
Fraction processing
Centrifugation
Pelleting: volume per fraction
6 ml
Pelleting: duration (min)
65
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
EV-subtype
Distinction between multiple subtypes
sedimentation speed;Other
Used subtypes
100,000g
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ HSP70
Characterization: Lipid analysis
No
EM
EM-type
Cryo-EM
Image type
Wide-field
|
||||||||
EV190019 | 3/4 | Mus musculus | C2C12 |
DG (d)(U)C |
Leermakers PA | 2019 | 44% | |
Study summaryFull title
All authors
Leermakers PA, Remels AHV, Zonneveld MI, Rouschop KMA, Schols AMWJ, Gosker HR.
Journal
FASEB J
Abstract
Iron homeostasis is essential for mitochondrial function, and iron deficiency has been associated wi (show more...)
EV-METRIC
44% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Deferiprone/iron chelation
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: HSP70/ Flotillin1
non-EV: Proteomics
no
EV density (g/ml)
1.12-1.19 g/ml
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)/Mechanism of uptake/transfer
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
C2C12
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Density gradient
Type
Continuous
Lowest density fraction
0.4M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
12.4
Sample volume (mL)
0.2
Orientation
Bottom-up
Rotor type
SW 41 Ti
Speed (g)
188000
Duration (min)
16
Fraction volume (mL)
1 ml
Fraction processing
Centrifugation
Pelleting: volume per fraction
6 ml
Pelleting: duration (min)
65
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
EV-subtype
Distinction between multiple subtypes
sedimentation speed;Other
Used subtypes
16,000g
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ HSP70
Characterization: Lipid analysis
No
EM
EM-type
Cryo-EM
Image type
Wide-field
|
||||||||
EV190019 | 4/4 | Mus musculus | C2C12 |
DG (d)(U)C |
Leermakers PA | 2019 | 44% | |
Study summaryFull title
All authors
Leermakers PA, Remels AHV, Zonneveld MI, Rouschop KMA, Schols AMWJ, Gosker HR.
Journal
FASEB J
Abstract
Iron homeostasis is essential for mitochondrial function, and iron deficiency has been associated wi (show more...)
EV-METRIC
44% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Deferiprone/iron chelation
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: HSP70/ Flotillin1
non-EV: Proteomics
no
EV density (g/ml)
1.12-1.19 g/ml
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)/Mechanism of uptake/transfer
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
C2C12
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Density gradient
Type
Continuous
Lowest density fraction
0.4M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
12.4
Sample volume (mL)
0.2
Orientation
Bottom-up
Rotor type
SW 41 Ti
Speed (g)
188000
Duration (min)
16
Fraction volume (mL)
1 ml
Fraction processing
Centrifugation
Pelleting: volume per fraction
6 ml
Pelleting: duration (min)
65
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
EV-subtype
Distinction between multiple subtypes
sedimentation speed;Other
Used subtypes
100,000g
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ HSP70
Characterization: Lipid analysis
No
EM
EM-type
Cryo-EM
Image type
Wide-field
|
||||||||
1 - 4 of 4 |
EV-TRACK ID | EV190019 | |||
---|---|---|---|---|
species | Mus musculus | |||
sample type | Cell culture | |||
cell type | C2C12 | |||
condition | Control condition | Control condition | Deferiprone/iron chelation | Deferiprone/iron chelation |
separation protocol | DG (d)(U)C | DG (d)(U)C | DG (d)(U)C | DG (d)(U)C |
EV subtype | 16 000g | 100 000g | 16 000g | 100 000g |
Exp. nr. | 1 | 2 | 3 | 4 |
EV-METRIC % | 44 | 44 | 44 | 44 |