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You searched for: EV180004 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV180004 | 1/1 | Homo sapiens | Blood plasma |
(d)(U)C qEV |
Picciolini S | 2018 | 37% | |
Study summaryFull title
All authors
Picciolini S, Gualerzi A, Vanna R, Sguassero A, Gramatica F, Bedoni M, Masserini M, Morasso C
Journal
Anal Chem
Abstract
The use of exosomes for diagnostic and disease monitoring purposes is becoming particularly appealin (show more...)
EV-METRIC
37% (70th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
qEV Protein markers
EV: CD81/ ephrinB/ CD171/ PLP1/ Flotillin-1/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
No
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
59.77
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD81, Flotillin-1
Other 1
Surface plasmon resonance imaging
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
150
EV concentration
Yes
Particle yield
1500000000
EM
EM-type
Transmission-EM
Image type
Wide-field
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1 - 1 of 1 |
EV-TRACK ID | EV180004 |
---|---|
species | Homo sapiens |
sample type | Blood plasma |
condition | Control condition |
separation protocol | (d)(U)C qEV |
Exp. nr. | 1 |
EV-METRIC % | 37 |