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You searched for: EV170070 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV170070 | 1/1 | Homo sapiens | CEPH/UTAH family PEDIGREE 1463 peripheral blood B lymphocyte |
(d)(U)C Filtration |
Tsang EK | 2017 | 33% | |
Study summaryFull title
All authors
Tsang EK, Abell NS, Li X, Anaya V, Karczewski KJ, Knowles DA, Sierra RG, Smith KS, Montgomery SB.
Journal
G3 (Bethesda)
Abstract
Exosomes are small extracellular vesicles that carry heterogeneous cargo, including RNA, between cel (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Epstein-Barr virus-transformed
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: HSP70
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
CEPH/UTAH family PEDIGREE 1463 peripheral blood B lymphocyte
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
120000
Wash: time (min)
70
Wash: Rotor Type
TLA-100.3
Wash: speed (g)
120000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
HSP70
Not detected contaminants
Calnexin
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
107
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
100
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1 - 1 of 1 |
EV-TRACK ID | EV170070 |
---|---|
species | Homo sapiens |
sample type | Cell culture |
cell type | CEPH/UTAH family PEDIGREE 1463 peripheral blood B lymphocyte |
condition | Epstein-Barr virus-transformed |
separation protocol | (d)(U)C Filtration |
Exp. nr. | 1 |
EV-METRIC % | 33 |