Search > Results
|Details||EV-TRACK code||Experiment nr.||Species||Sample type||Separation protocol||First author||Year||EV-METRIC|
Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine.
Oeyen E, Van Mol K, Baggerman G, Willems H, Boonen K, Rolfo C, Pauwels P, Jacobs A, Schildermans K, Cho WC, Mertens I.
J Extracell Vesicles
Extracellular vesicles (EVs) have a great potential in clinical applications. However, their isolati (show more...)Extracellular vesicles (EVs) have a great potential in clinical applications. However, their isolation from different bodily fluids and their characterisation are currently not optimal or standardised. Here, we report the results of examining the performance of ultrafiltration combined with size exclusion chromatography (UF-SEC) to isolate EVs from urine. The results reveal that UF-SEC is an efficient method and provides high purity. Furthermore, we introduce asymmetrical-flow field-flow fractionation coupled with a UV detector and multi-angle light-scattering detector (AF4/UV-MALS) as a characterisation method and compare it with current methods. We demonstrate that AF4/UV-MALS is a straightforward and reproducible method for determining size, amount and purity of isolated urinary EVs. (hide)
62% (92nd percentile of all experiments on the same sample type)
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
Separation method: density gradient, at least as validation of results attributed to EVs
Separation method: reporting of obtained EV density
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
Protein analysis: antibody clone/reference number and dilution
Protein analysis: lysis buffer composition
Show all info
Identification of content (omics approaches), Technical analysis comparing/optimizing EV-related methods
Cut-off size (kDa)
Characterization: Protein analysis
Protein Concentration Method
Antibody details provided?
Lysis buffer provided?
Detected EV-associated proteins
Characterization: Lipid analysis
Characterization: Particle analysis
Reported size (nm)
1.80E+09 particles/ml start sample
Report size (nm)
Other particle analysis name(1)
Asymmetrical flow field-flow fractionation
|1 - 1 of 1|