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You searched for: EV150046 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV150046 | 2/2 | Mus musculus | Serum |
(d)(U)C ExoQuick Filtration |
Momen-Heravi F | 2015 | 25% | |
Study summaryFull title
All authors
Momen-Heravi F, Saha B, Kodys K, Catalano D, Satishchandran A, Szabo G
Journal
J Transl Med
Abstract
BACKGROUND: It has been well documented that alcohol and its metabolites induce injury and inflammat (show more...)
EV-METRIC
25% (67th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Filtration Protein markers
EV: CD63
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Mus musculus
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Filtration steps
> 0.45 µm, 0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
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EV150046 | 1/2 | Homo sapiens | Blood plasma |
(d)(U)C ExoQuick Filtration |
Momen-Heravi F | 2015 | 0% | |
Study summaryFull title
All authors
Momen-Heravi F, Saha B, Kodys K, Catalano D, Satishchandran A, Szabo G
Journal
J Transl Med
Abstract
BACKGROUND: It has been well documented that alcohol and its metabolites induce injury and inflammat (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Filtration Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Filtration steps
> 0.45 µm, 0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Particle analysis
None
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1 - 2 of 2 |
EV-TRACK ID | EV150046 | |
---|---|---|
species | Mus musculus | Homo sapiens |
sample type | Serum | Blood plasma |
condition | NAY | NAY |
separation protocol | (d)(U)C ExoQuick Filtration | (d)(U)C ExoQuick Filtration |
Exp. nr. | 2 | 1 |
EV-METRIC % | 25 | 0 |