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You searched for: EV140117 (EV-TRACK ID)
Showing 1 - 8 of 8
Showing 1 - 8 of 8
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140117 | 2/8 | Homo sapiens | Blood plasma | (d)(U)C | He M | 2014 | 44% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
44% (77th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD81/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD63/ CD81/ CD9
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140117 | 3/8 | Homo sapiens | Blood plasma |
IAF Microfluidics |
He M | 2014 | 33% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
33% (66th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF
Microfluidics Protein markers
EV:
non-EV: Proteomics
no
TEM measurements
50-100
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
CD81
Other
Name other separation method
Microfluidics
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
Report size (nm)
50-100
|
||||||||
EV140117 | 4/8 | Homo sapiens | Blood plasma |
IAF Microfluidics |
He M | 2014 | 33% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
33% (66th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF
Microfluidics Protein markers
EV:
non-EV: Proteomics
no
TEM measurements
25-100
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
CD9
Other
Name other separation method
Microfluidics
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
Report size (nm)
25-100
|
||||||||
EV140117 | 8/8 | Homo sapiens | Blood plasma |
IAF Microfluidics |
He M | 2014 | 33% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
33% (66th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF
Microfluidics Protein markers
EV:
non-EV: Proteomics
no
TEM measurements
50-100
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
α-IGF-1R
Other
Name other separation method
Microfluidics
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
Report size (nm)
50-100
|
||||||||
EV140117 | 1/8 | Homo sapiens | NAY | (d)(U)C | He M | 2014 | 22% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
22% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD81/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD63/ CD81/ CD9
Characterization: Particle analysis
None
|
||||||||
EV140117 | 6/8 | Homo sapiens | Blood plasma |
IAF Microfluidics |
He M | 2014 | 17% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
17% (46th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF
Microfluidics Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
CA125
Other
Name other separation method
Microfluidics
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140117 | 7/8 | Homo sapiens | Blood plasma |
IAF Microfluidics |
He M | 2014 | 17% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
17% (46th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF
Microfluidics Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
EpCAM
Other
Name other separation method
Microfluidics
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140117 | 5/8 | Homo sapiens | Blood plasma |
IAF Microfluidics |
He M | 2014 | 0% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF
Microfluidics Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
CD63
Other
Name other separation method
Microfluidics
Characterization: Particle analysis
None
|
||||||||
1 - 8 of 8 |
EV-TRACK ID | EV140117 | |||||||
---|---|---|---|---|---|---|---|---|
species | Homo sapiens | |||||||
sample type | Blood plasma | Blood plasma | Blood plasma | Blood plasma | Cell culture | Blood plasma | Blood plasma | Blood plasma |
cell type | NA | NA | NA | NA | NAY | NA | NA | NA |
condition | NAY | NAY | NAY | NAY | NAY | NAY | NAY | NAY |
separation protocol | (d)(U)C | IAF Microfluidics | IAF Microfluidics | IAF Microfluidics | (d)(U)C | IAF Microfluidics | IAF Microfluidics | IAF Microfluidics |
Exp. nr. | 2 | 3 | 4 | 8 | 1 | 6 | 7 | 5 |
EV-METRIC % | 44 | 33 | 33 | 33 | 22 | 17 | 17 | 0 |