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You searched for: EV140101 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140101 | 1/1 | Gallus gallus | Bile |
(d)(U)C DG Filtration |
Wang Y | 2014 | 71% | |
Study summaryFull title
All authors
Wang Y, Wang G, Wang Z, Zhang H, Zhang L, Cheng Z
Journal
Biochem Cell Biol
Abstract
Exosomes, which are small membrane vesicles of endocytic origin, carry lipids, RNA/miRNAs, and prote (show more...)
EV-METRIC
71% (91st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bile
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Adj. k-factor
235.7 (pelleting) / 235.7 (washing)
Protein markers
EV:
non-EV: Proteomics
yes
EV density (g/ml)
1.13-1.19
Show all info
Study aim
Function
Sample
Species
Gallus gallus
Sample Type
Bile
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
P28S
Pelleting: adjusted k-factor
235.7
Wash: Rotor Type
P28S
Wash: adjusted k-factor
235.7
Density gradient
Lowest density fraction
8
Highest density fraction
45
Orientation
Top-down
Rotor type
P28S
Speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
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1 - 1 of 1 |
EV-TRACK ID | EV140101 |
---|---|
species | Gallus gallus |
sample type | Bile |
condition | NAY |
separation protocol | (d)(U)C DG Filtration |
Exp. nr. | 1 |
EV-METRIC % | 71 |