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You searched for: EV140031 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140031 | 1/2 | Homo sapiens | Urine |
(d)(U)C UF |
Kosanovi? M | 2014 | 44% | |
Study summaryFull title
All authors
Kosanovic M, Jankovic M
Journal
Biotechniques
Abstract
Urine is a readily available source of relatively large quantities of extracellular vesicles (EVs). (show more...)
EV-METRIC
44% (80th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes / extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
UF Adj. k-factor
157.1 (pelleting)
Protein markers
EV: CD63/ Galectin3
non-EV: Tamm-Horsfall glycoprotein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
50.2Ti
Pelleting: adjusted k-factor
157.1
Wash: volume per pellet (ml)
6
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD63/ Galectin3
Detected contaminants
Tamm-Horsfall glycoprotein
ELISA
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD63/ Galectin3
Detected contaminants
Tamm-Horsfall glycoprotein
|
||||||||
EV140031 | 2/2 | Homo sapiens | Urine |
(d)(U)C Filtration UF |
Kosanovi? M | 2014 | 44% | |
Study summaryFull title
All authors
Kosanovic M, Jankovic M
Journal
Biotechniques
Abstract
Urine is a readily available source of relatively large quantities of extracellular vesicles (EVs). (show more...)
EV-METRIC
44% (80th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes / extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Adj. k-factor
157.1 (pelleting)
Protein markers
EV: CD63/ Galectin3
non-EV: Tamm-Horsfall glycoprotein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
50.2Ti
Pelleting: adjusted k-factor
157.1
Wash: volume per pellet (ml)
6
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD63/ Galectin3
Detected contaminants
Tamm-Horsfall glycoprotein
ELISA
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD63/ Galectin3
Detected contaminants
Tamm-Horsfall glycoprotein
Characterization: Particle analysis
EM
EM-type
transmission EM/ scanning EM
Image type
Wide-field
Report size (nm)
Not reported
|
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1 - 2 of 2 |
EV-TRACK ID | EV140031 | |
---|---|---|
species | Homo sapiens | |
sample type | Urine | |
condition | NAY | |
separation protocol | (d)(U)C UF | (d)(U)C Filtration UF |
Exp. nr. | 1 | 2 |
EV-METRIC % | 44 | 44 |