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You searched for: EV140029 (EV-TRACK ID)
Showing 1 - 12 of 12
Showing 1 - 12 of 12
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140029 | 1/12 | Homo sapiens | NAY | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
475.5 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
475.5
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 2/12 | Homo sapiens | NAY | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
234.2 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
234.2
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 3/12 | Homo sapiens | NAY | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
156.9 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
156.9
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 4/12 | Homo sapiens | NAY | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
117.9 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
117.9
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 5/12 | Homo sapiens | NAY | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
93.96 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
93.96
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 6/12 | Homo sapiens | NAY | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
78.45 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
78.45
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 7/12 | Homo sapiens | NAY | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
475.5 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
475.5
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 8/12 | Homo sapiens | NAY | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
234.2 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
234.2
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 9/12 | Homo sapiens | NAY | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
156.9 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
156.9
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 10/12 | Homo sapiens | NAY | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
117.9 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
117.9
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 11/12 | Homo sapiens | NAY | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
93.96 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Equal to or above 150,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
93.96
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 12/12 | Homo sapiens | NAY | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
78.45 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Equal to or above 150,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
78.45
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
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EV-TRACK ID | EV140029 | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
species | Homo sapiens | |||||||||||
sample type | Cell culture | |||||||||||
cell type | NAY | |||||||||||
condition | NAY | |||||||||||
separation protocol | (d)(U)C | (d)(U)C | (d)(U)C | (d)(U)C | (d)(U)C | (d)(U)C | (d)(U)C | (d)(U)C | (d)(U)C | (d)(U)C | (d)(U)C | (d)(U)C |
Exp. nr. | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
EV-METRIC % | 44 | 44 | 44 | 44 | 44 | 44 | 44 | 44 | 44 | 44 | 44 | 44 |