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You searched for: EV130153 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130153 | 2/3 | Homo sapiens | Urine |
(d)(U)C DG |
Oosthuyzen W | 2013 | 33% | |
Study summaryFull title
All authors
Oosthuyzen W, Sime NE, Ivy JR, Turtle EJ, Street JM, Pound J, Bath LE, Webb DJ, Gregory CD, Bailey MA, Dear JW
Journal
J Physiol
Abstract
Exosomes are vesicles that are released from the kidney into urine. They contain protein and RNA fro (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: TSG101/ Flotilin1/ CD24
non-EV: Proteomics
no
EV density (g/ml)
1.12-1.16
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Top-down
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotilin1/ TSG101/ CD24
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD24
|
||||||||
EV130153 | 1/3 | Homo sapiens | Urine | (d)(U)C | Oosthuyzen W | 2013 | 0% | |
Study summaryFull title
All authors
Oosthuyzen W, Sime NE, Ivy JR, Turtle EJ, Street JM, Pound J, Bath LE, Webb DJ, Gregory CD, Bailey MA, Dear JW
Journal
J Physiol
Abstract
Exosomes are vesicles that are released from the kidney into urine. They contain protein and RNA fro (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Characterization: Particle analysis
NTA
|
||||||||
EV130153 | 3/3 | Homo sapiens | Urine |
(d)(U)C ExoQuick |
Oosthuyzen W | 2013 | 0% | |
Study summaryFull title
All authors
Oosthuyzen W, Sime NE, Ivy JR, Turtle EJ, Street JM, Pound J, Bath LE, Webb DJ, Gregory CD, Bailey MA, Dear JW
Journal
J Physiol
Abstract
Exosomes are vesicles that are released from the kidney into urine. They contain protein and RNA fro (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
No
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Particle analysis
NTA
|
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1 - 3 of 3 |
EV-TRACK ID | EV130153 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Urine | ||
condition | NAY | ||
separation protocol | (d)(U)C DG | (d)(U)C | (d)(U)C ExoQuick |
Exp. nr. | 2 | 1 | 3 |
EV-METRIC % | 33 | 0 | 0 |