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You searched for: EV130032 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130032 | 1/1 | Homo sapiens | NAY |
(d)(U)C DG Filtration UF |
Bernhard OK | 2013 | 38% | |
Study summaryFull title
All authors
Bernhard OK, Greening DW, Barnes TW, Ji H, Simpson RJ
Journal
Biochim Biophys Acta Proteins Proteom
Abstract
Colorectal cancer (CRC), one of the most prevalent cancers in the western world, is treatable if det (show more...)
EV-METRIC
38% (80th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Filtration UF Protein markers
EV: CD9
non-EV: Proteomics
no
EV density (g/ml)
1.110
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Density gradient
Lowest density fraction
5
Highest density fraction
40
Orientation
Top-down
Speed (g)
100000
Filtration steps
0.2µm > x > 0.1µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9
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1 - 1 of 1 |
EV-TRACK ID | EV130032 |
---|---|
species | Homo sapiens |
sample type | Cell culture |
cell type | NAY |
condition | NAY |
separation protocol | (d)(U)C DG Filtration UF |
Exp. nr. | 1 |
EV-METRIC % | 38 |