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You searched for: EV120108 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120108 | 1/2 | Mesocricetus auratus | NAY |
(d)(U)C DC |
Wik L | 2012 | 25% | |
Study summaryFull title
All authors
Wik L, Klingeborn M, Willander H, Linne T
Journal
Prion
Abstract
The cellular prion protein (PrP (C) ) is attached to the cell membrane via its glycosylphosphatidyli (show more...)
EV-METRIC
25% (64th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
DC Protein markers
EV: TSG101/ Actin
non-EV: Proteomics
no
Show all info
Study aim
Other/The shedding and release mechanism of prion proteins
Sample
Species
Mesocricetus auratus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TSG101/ Actin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Actin
Characterization: Particle analysis
EM
EM-type
immune EM/ scanning EM
EM protein
PrP; BLV-gp51
Image type
Close-up, Wide-field
Report size (nm)
Not reported
|
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EV120108 | 2/2 | Mesocricetus auratus | NAY |
(d)(U)C DC |
Wik L | 2012 | 0% | |
Study summaryFull title
All authors
Wik L, Klingeborn M, Willander H, Linne T
Journal
Prion
Abstract
The cellular prion protein (PrP (C) ) is attached to the cell membrane via its glycosylphosphatidyli (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
DC Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Other/The shedding and release mechanism of prion proteins
Sample
Species
Mesocricetus auratus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
No
Characterization: Particle analysis
None
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1 - 2 of 2 |
EV-TRACK ID | EV120108 | |
---|---|---|
species | Mesocricetus auratus | |
sample type | Cell culture | |
cell type | NAY | |
condition | NAY | |
separation protocol | (d)(U)C DC | (d)(U)C DC |
Exp. nr. | 1 | 2 |
EV-METRIC % | 25 | 0 |