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You searched for: EV120089 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120089 | 1/1 | Borrelia burgdorferi | Bacteria |
(d)(U)C DG Filtration |
Toledo A | 2012 | 22% | |
Study summaryFull title
All authors
Toledo A, Coleman JL, Kuhlow CJ, Crowley JT, Benach JL
Journal
Infect Immun
Abstract
The agent of Lyme disease, Borrelia burgdorferi, has a number of outer membrane proteins that are di (show more...)
EV-METRIC
22% (71st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bacteria
Sample origin
NAY
Focus vesicles
OMV
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV: OspA/ Enolase
non-EV: DnaK Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Borrelia burgdorferi
Sample Type
Bacteria
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
15
Highest density fraction
40
Orientation
Bottom-up
Speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
"OspA/ Enolase"
Detected contaminants
DnaK
ELISA
Antibody details provided?
No
Detected EV-associated proteins
"OspA/ Enolase"
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EV-TRACK ID | EV120089 |
---|---|
species | Borrelia burgdorferi |
sample type | Bacteria |
condition | NAY |
separation protocol | (d)(U)C DG Filtration |
Exp. nr. | 1 |
EV-METRIC % | 22 |