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You searched for: EV120045 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120045 | 1/1 | Homo sapiens | Urine |
(d)(U)C UF |
Sun AL | 2012 | 25% | |
Study summaryFull title
All authors
Sun AL, Deng JT, Guan GJ, Chen SH, Liu YT, Cheng J, Li ZW, Zhuang XH, Sun FD, Deng HP
Journal
Diab Vasc Dis Res
Abstract
The present study was designed to identify the changes in microvesicle-dipeptidyl peptidase-IV (DPP (show more...)
EV-METRIC
25% (56th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
UF Protein markers
EV: AD-1/ DPP IV
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
No
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
"AD-1/ DPP IV"
ELISA
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
"AD-1/ DPP IV"
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1 - 1 of 1 |
EV-TRACK ID | EV120045 |
---|---|
species | Homo sapiens |
sample type | Urine |
condition | NAY |
separation protocol | (d)(U)C UF |
Exp. nr. | 1 |
EV-METRIC % | 25 |