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You searched for: EV120038 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120038 | 2/2 | Mus musculus | Brain tissue |
(d)(U)C DG Filtration |
Perez-Gonzalez R | 2012 | 56% | |
Study summaryFull title
All authors
Perez-Gonzalez R, Gauthier SA, Kumar A, Levy E
Journal
J Biol Chem
Abstract
In vitro studies have shown that neuronal cell cultures secrete exosomes containing amyloid-? precur (show more...)
EV-METRIC
56% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Brain tissue
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV: NISCASTRIN/ BACE1/ Beta-amyloid/ APP/ Flotilin1/ ADAM10/ AChE
non-EV: Proteomics
no
EV density (g/ml)
1.07-1.17
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Mus musculus
Sample Type
Brain tissue
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Wash: volume per pellet (ml)
60
Density gradient
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Bottom-up
Speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotilin1/ ADAM10/ BACE1/ NISCASTRIN/ APP/ Beta-amyloid/ AChE
ELISA
Antibody details provided?
No
Detected EV-associated proteins
ADAM10/ BACE1/ NISCASTRIN/ APP/ Beta-amyloid/ AChE
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
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EV120038 | 1/2 | Homo sapiens | Brain tissue |
(d)(U)C Filtration |
Perez-Gonzalez R | 2012 | 29% | |
Study summaryFull title
All authors
Perez-Gonzalez R, Gauthier SA, Kumar A, Levy E
Journal
J Biol Chem
Abstract
In vitro studies have shown that neuronal cell cultures secrete exosomes containing amyloid-? precur (show more...)
EV-METRIC
29% (29th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Brain tissue
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Homo sapiens
Sample Type
Brain tissue
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Wash: volume per pellet (ml)
60
Filtration steps
0.22µm or 0.2µm
Characterization: Particle analysis
EM
EM-type
immune EM
EM protein
Flotillin; Tsg101;APP;Beta-amyloid
Image type
Close-up, Wide-field
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1 - 2 of 2 |
EV-TRACK ID | EV120038 | |
---|---|---|
species | Mus musculus | Homo sapiens |
sample type | Brain tissue | Brain tissue |
condition | NAY | NAY |
separation protocol | (d)(U)C DG Filtration | (d)(U)C Filtration |
Exp. nr. | 2 | 1 |
EV-METRIC % | 56 | 29 |