Search > Results
You searched for: EV120023 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120023 | 1/3 | Homo sapiens | NAY |
(d)(U)C DG Filtration |
Palma J | 2012 | 57% | |
Study summaryFull title
All authors
Palma J, Yaddanapudi SC, Pigati L, Havens MA, Jeong S, Weiner GA, Weimer KM, Stern B, Hastings ML, Duelli DM
Journal
Nucleic Acids Res
Abstract
MicroRNAs (miRNAs) are released from cells in association with proteins or microvesicles. We previou (show more...)
EV-METRIC
57% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Particles
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV:
non-EV: Proteomics
no
EV density (g/ml)
1.13-1.19
TEM measurements
86
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Lowest density fraction
0.25
Highest density fraction
2.5
Orientation
Bottom-up
Speed (g)
100000
Filtration steps
0.45µm > x > 0.22µm,
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
Report size (nm)
86
|
||||||||
EV120023 | 2/3 | Homo sapiens | NAY |
(d)(U)C DG Filtration |
Palma J | 2012 | 43% | |
Study summaryFull title
All authors
Palma J, Yaddanapudi SC, Pigati L, Havens MA, Jeong S, Weiner GA, Weimer KM, Stern B, Hastings ML, Duelli DM
Journal
Nucleic Acids Res
Abstract
MicroRNAs (miRNAs) are released from cells in association with proteins or microvesicles. We previou (show more...)
EV-METRIC
43% (81st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Particles
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV:
non-EV: Proteomics
no
EV density (g/ml)
1.13-1.19
TEM measurements
58-72
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Lowest density fraction
0.25
Highest density fraction
2.5
Orientation
Bottom-up
Speed (g)
100000
Filtration steps
0.45µm > x > 0.22µm,
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
Report size (nm)
58-72
|
||||||||
EV120023 | 3/3 | Homo sapiens | Milk |
(d)(U)C DG Filtration |
Palma J | 2012 | 43% | |
Study summaryFull title
All authors
Palma J, Yaddanapudi SC, Pigati L, Havens MA, Jeong S, Weiner GA, Weimer KM, Stern B, Hastings ML, Duelli DM
Journal
Nucleic Acids Res
Abstract
MicroRNAs (miRNAs) are released from cells in association with proteins or microvesicles. We previou (show more...)
EV-METRIC
43% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Milk
Sample origin
NAY
Focus vesicles
Particles
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV:
non-EV: Proteomics
no
EV density (g/ml)
1.15;1.25
TEM measurements
130-180
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Lowest density fraction
0.25
Highest density fraction
2.5
Orientation
Bottom-up
Speed (g)
100000
Filtration steps
0.45µm > x > 0.22µm,
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
Report size (nm)
130-180
|
||||||||
1 - 3 of 3 |
EV-TRACK ID | EV120023 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Cell culture | Cell culture | Milk |
cell type | NAY | NAY | NA |
medium | serum free | serum free | |
condition | NAY | NAY | NAY |
separation protocol | (d)(U)C DG Filtration | (d)(U)C DG Filtration | (d)(U)C DG Filtration |
Exp. nr. | 1 | 2 | 3 |
EV-METRIC % | 57 | 43 | 43 |