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You searched for: EV120022 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120022 | 1/2 | Homo sapiens | Placental explant |
(d)(U)C DG |
Kshirsagar SK | 2012 | 56% | |
Study summaryFull title
All authors
Kshirsagar SK, Alam SM, Jasti S, Hodes H, Nauser T, Gilliam M, Billstrand C, Hunt JS, Petroff MG
Journal
Placenta
Abstract
The semiallogenic fetus is tolerated by the maternal immune system through control of innate and ada (show more...)
EV-METRIC
56% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Placental explant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: B7H-1/ B7H-3/ CD63/ LAMP1/ MHC1
non-EV: Cell organelle protein Proteomics
no
EV density (g/ml)
1.15-1.18
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Placental explant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Top-down
Rotor type
TLA100.4
Speed (g)
110000
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ LAMP1/ MHC1/ B7H-1/ B7H-3
Detected contaminants
Cell organelle protein
ELISA
Antibody details provided?
No
Detected EV-associated proteins
LAMP1/ MHC1/ B7H-1/ B7H-3
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
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EV120022 | 2/2 | Homo sapiens | Trophoblasts; PMBC | (d)(U)C | Kshirsagar SK | 2012 | 11% | |
Study summaryFull title
All authors
Kshirsagar SK, Alam SM, Jasti S, Hodes H, Nauser T, Gilliam M, Billstrand C, Hunt JS, Petroff MG
Journal
Placenta
Abstract
The semiallogenic fetus is tolerated by the maternal immune system through control of innate and ada (show more...)
EV-METRIC
11% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Trophoblasts; PMBC
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: B7H-1/ CD63/ LAMP1/ MHC1
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Trophoblasts; PMBC
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ LAMP1/ MHC1/ B7H-1
ELISA
Antibody details provided?
No
Detected EV-associated proteins
LAMP1/ MHC1/ B7H-1
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
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1 - 2 of 2 |
EV-TRACK ID | EV120022 | |
---|---|---|
species | Homo sapiens | |
sample type | Placental explant | Trophoblasts PMBC |
condition | NAY | NAY |
separation protocol | (d)(U)C DG | (d)(U)C |
Exp. nr. | 1 | 2 |
EV-METRIC % | 56 | 11 |