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You searched for: EV120008 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120008 | 2/2 | Mus musculus | NAY |
(d)(U)C DG UF |
Montecalvo A | 2012 | 63% | |
Study summaryFull title
All authors
Montecalvo A, Larregina AT, Shufesky WJ, Stolz DB, Sullivan ML, Karlsson JM, Baty CJ, Gibson GA, Erdos G, Wang Z, Milosevic J, Tkacheva OA, Divito SJ, Jordan R, Lyons-Weiler J, Watkins SC, Morelli AE
Journal
Blood
Abstract
Dendritic cells (DCs) are the most potent APCs. Whereas immature DCs down-regulate T-cell responses (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG UF Protein markers
EV: CD54/ CD81/ CD86/ MHC2/ CD9/ MHC1
non-EV: Proteomics
no
EV density (g/ml)
1.166-1.199
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
No
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Bottom-up
Speed (g)
200000
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ CD9/ MHC1/ MHC2/ CD86/ CD54
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD81/ CD9/ MHC1/ MHC2/ CD86/ CD54
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120008 | 1/2 | Mus musculus | NAY |
(d)(U)C DC UF |
Montecalvo A | 2012 | 38% | |
Study summaryFull title
All authors
Montecalvo A, Larregina AT, Shufesky WJ, Stolz DB, Sullivan ML, Karlsson JM, Baty CJ, Gibson GA, Erdos G, Wang Z, Milosevic J, Tkacheva OA, Divito SJ, Jordan R, Lyons-Weiler J, Watkins SC, Morelli AE
Journal
Blood
Abstract
Dendritic cells (DCs) are the most potent APCs. Whereas immature DCs down-regulate T-cell responses (show more...)
EV-METRIC
38% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DC UF Protein markers
EV: CD54/ CD81/ CD86/ MHC2/ CD9/ MHC1
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
No
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ CD9/ MHC1/ MHC2/ CD86/ CD54
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD81/ CD9/ MHC1/ MHC2/ CD86/ CD54
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
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1 - 2 of 2 |
EV-TRACK ID | EV120008 | |
---|---|---|
species | Mus musculus | |
sample type | Cell culture | |
cell type | NAY | |
condition | NAY | |
separation protocol | (d)(U)C DG UF | (d)(U)C DC UF |
Exp. nr. | 2 | 1 |
EV-METRIC % | 63 | 38 |