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You searched for: EV110046 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV110046 | 2/2 | Homo sapiens | NAY |
(d)(U)C DG Filtration |
Koumangoye RB | 2011 | 22% | |
Study summaryFull title
All authors
Koumangoye RB, Sakwe AM, Goodwin JS, Patel T, Ochieng J
Journal
PLoS One
Abstract
Exosomes are nano-vesicles secreted by a wide range of mammalian cell types. These vesicles are abun (show more...)
EV-METRIC
22% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV: HSP90/ CD63/ LAMP1
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
10
Highest density fraction
60
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ HSP90/ LAMP1
ELISA
Antibody details provided?
No
Detected EV-associated proteins
LAMP1
|
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EV110046 | 1/2 | Homo sapiens | Serum | (d)(U)C | Koumangoye RB | 2011 | 0% | |
Study summaryFull title
All authors
Koumangoye RB, Sakwe AM, Goodwin JS, Patel T, Ochieng J
Journal
PLoS One
Abstract
Exosomes are nano-vesicles secreted by a wide range of mammalian cell types. These vesicles are abun (show more...)
EV-METRIC
0% (median: 13% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
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1 - 2 of 2 |
EV-TRACK ID | EV110046 | |
---|---|---|
species | Homo sapiens | |
sample type | Cell culture | Serum |
cell type | NAY | NA |
medium | EV Depleted | |
condition | NAY | NAY |
separation protocol | (d)(U)C DG Filtration | (d)(U)C |
Exp. nr. | 2 | 1 |
EV-METRIC % | 22 | 0 |