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You searched for: 2014 (Year of publication)
Showing 51 - 100 of 668
Showing 51 - 100 of 668
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140047 | 2/2 | Homo sapiens | Cell culture supernatant | (d)(U)C Filtration |
Gonzalez E | 2014 | 44% | |
Study summaryFull title
All authors
Gonzalez E, Piva M, Rodriguez-Suarez E, Gil D, Royo F, Elortza F, Falcon-Perez JM, Vivanco Md
Journal
PLoS One
Abstract
Breast cancer is a leading cause of cancer-associated death worldwide. One of the most important pro (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C + Filtration
Protein markers
EV: CD63/ CD133/ MFGE8/ CD81/ Flotilin1/ Annexin2/ CD13
non-EV: Cell organelle protein Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
60
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD81/ Flotilin1/ MFGE8/ CD13/ CD133/ Annexin2
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
MFGE8/ CD13/ CD133/ Annexin2
Characterization: Particle analysis
NTA
EM
EM-type
cryo EM
Image type
Close-up
|
||||||||
EV140111 | 1/2 | Homo sapiens | Cell culture supernatant | (d)(U)C | Cypryk W | 2014 | 44% | |
Study summaryFull title
All authors
Cypryk W, Ohman T, Eskelinen EL, Matikainen S, Nyman TA
Journal
J Proteome Res
Abstract
Fungal infections (mycoses) are common diseases of varying severity that cause problems, especially (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
295 (pelleting)
Protein markers
EV: Annexin1/ Tubulin/ TSG101/ Alix
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
60
Pelleting: rotor type
SW28
Pelleting: adjusted k-factor
295.0
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ TSG101/ Annexin1/ Tubulin
ELISA
Detected EV-associated proteins
Annexin1/ Tubulin
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM/ immune EM
Proteïns
Annexin1 ; 14-3-3beta
Image type
Close-up, Wide-field
|
||||||||
EV140110 | 2/2 | Mus musculus | Cell culture supernatant | DG (d)(U)C |
Cossetti C | 2014 | 44% | |
Study summaryFull title
All authors
Cossetti C, Iraci N, Mercer TR, Leonardi T, Alpi E, Drago D, Alfaro-Cervello C, Saini HK, Davis MP, Schaeffer J, Vega B, Stefanini M, Zhao C, Muller W, Garcia-Verdugo JM, Mathivanan S, Bachi A, Enright AJ, Mattick JS, Pluchino S
Journal
Mol Cell
Abstract
The idea that stem cell therapies work only via cell replacement is challenged by the observation of (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
DG + (d)(U)C
Adj. k-factor
122.2 (pelleting) / 89.21 (washing)
Protein markers
EV: TSG101/ CD63/ HSP90/ Alix/ Tnfr1/ HSP70/ Beta-actin/ Ago1/ CD9/ Ago2
non-EV: Proteomics
no
EV density (g/ml)
1.13-1.20
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
70
Pelleting: rotor type
70.1Ti
Pelleting: adjusted k-factor
122.2
Wash: Rotor Type
TLA55
Wash: adjusted k-factor
89.21
Density gradient
Density medium
Sucrose
Lowest density fraction
0.32
Highest density fraction
2
Orientation
Top-down
Rotor type
TLA55
Speed (g)
100000
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ CD9/ HSP90/ HSP70/ TSG101/ Ago1/ Ago2/ Tnfr1/ Beta-actin
ELISA
Detected EV-associated proteins
Ago1/ Ago2/ Tnfr1/ Beta-actin
Characterization: Particle analysis
NTA
|
||||||||
EV140108 | 1/1 | Acholeplasma laidlawii | Mycoplasma | DG (d)(U)C Filtration |
Chernov VM | 2014 | 44% | |
Study summaryFull title
All authors
Chernov VM, Mouzykantov AA, Baranova NB, Medvedeva ES, Grygorieva TY, Trushin MV, Vishnyakov IE, Sabantsev AV, Borchsenius SN, Chernova OA
Journal
J Proteomics
Abstract
Mycoplasmas (class Mollicutes), the smallest prokaryotes capable of self-replication, as well as Arc (show more...)
EV-METRIC
44% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Mycoplasma
Cell Name
DNF
Sample origin
DNF
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
DG + (d)(U)C + Filtration
Adj. k-factor
95.8 (pelleting)
Protein markers
EV: HSP20
non-EV: Proteomics
yes
Show all info
Study aim
Omics
Sample
Species
Acholeplasma laidlawii
Sample Type
Mycoplasma
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
60
Pelleting: rotor type
MLA80
Pelleting: adjusted k-factor
95.8
Density gradient
Density medium
Iodixanol
Lowest density fraction
10
Highest density fraction
40
Orientation
Top-down
Speed (g)
100000
Filtration steps
0.2µm > x > 0.1µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
HSP20
ELISA
Detected EV-associated proteins
HSP20
Characterization: Particle analysis
EM
EM-type
transmission EM/ immune EM/ scanning EM/ atomic force EM
Proteïns
HSP20
Image type
Close-up, Wide-field
|
||||||||
EV140238 | 3/3 | Mus musculus | Cell culture supernatant | DG (d)(U)C |
Yuyama K | 2014 | 44% | |
Study summaryFull title
All authors
Yuyama K, Sun H, Sakai S, Mitsutake S, Okada M, Tahara H, Furukawa J, Fujitani N, Shinohara Y, Igarashi Y
Journal
J Biol Chem
Abstract
Elevated levels of amyloid-? peptide (A?) in the human brain are linked to the pathogenesis of Alzhe (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C
Protein markers
EV: Alix/ GM1/ Flotilin1/ Actin/ Beta-amyloid
non-EV: Cell organelle protein Proteomics
no
EV density (g/ml)
1.12-1.16
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
60
Density gradient
Density medium
Sucrose
Lowest density fraction
0.25
Highest density fraction
2.2999999999999998
Orientation
Top-down
Speed (g)
100000
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ Flotilin1/ GM1/ Beta-amyloid/ Actin
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
GM1/ Beta-amyloid/ Actin
Characterization: Particle analysis
|
||||||||
EV140044 | 2/2 | Rattus norvegicus/rattus | Cell culture supernatant | (d)(U)C Filtration |
Wang X | 2014 | 44% | |
Study summaryFull title
All authors
Wang X, Huang W, Liu G, Cai W, Millard RW, Wang Y, Chang J, Peng T, Fan GC
Journal
J Mol Cell Cardiol
Abstract
Exosomes, nano-vesicles naturally released from living cells, have been well recognized to play crit (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C + Filtration
Protein markers
EV: CD81/ AChE/ CD63
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Rattus norvegicus/rattus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD81/ AChE
ELISA
Detected EV-associated proteins
AChE
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140043 | 1/3 | Homo sapiens | Cell culture supernatant | (d)(U)C Filtration |
Vargas A | 2014 | 44% | |
Study summaryFull title
All authors
Vargas A, Zhou S, Éthier-Chiasson M, Flipo D, Lafond J, Gilbert C, Barbeau B
Journal
FASEB J
Abstract
Exosomes are extracellular vesicles that mediate intercellular communication and are involved in sev (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C + Filtration
Protein markers
EV: TSG101/ AChE/ CD63
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
60
Wash: volume per pellet (ml)
10
Filtration steps
0.45µm > x > 0.22µm,
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ TSG101/ AChE
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
AChE
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140041 | 1/1 | Homo sapiens | Cell culture supernatant | DG (d)(U)C |
Toda Y | 2014 | 44% | |
Study summaryFull title
All authors
Toda Y, Takata K, Nakagawa Y, Kawakami H, Fujioka S, Kobayashi K, Hattori Y, Kitamura Y, Akaji K, Ashihara E
Journal
Biochem Biophys Res Commun
Abstract
Exosomes, the natural vehicles of various biological molecules, have been examined in several resear (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C
Protein markers
EV: TSG101/ CD63
non-EV: Proteomics
no
EV density (g/ml)
1.160
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Density gradient
Only used for validation of main results
1
Density medium
Sucrose
Lowest density fraction
0.25
Highest density fraction
2.5
Orientation
Bottom-up
Speed (g)
100000
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ TSG101
Characterization: Particle analysis
DLS
EM
EM-type
atomic force EM
Image type
Close-up, Wide-field
|
||||||||
EV140099 | 1/1 | Homo sapiens | Cell culture supernatant | (d)(U)C | Sinha A | 2014 | 44% | |
Study summaryFull title
All authors
Sinha A, Ignatchenko V, Ignatchenko A, Mejia-Guerrero S, Kislinger T
Journal
Biochem Biophys Res Commun
Abstract
Molecular communication between cancer cells and its stromal microenvironment is a key factor for ca (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
101.8 (pelleting) / 101.8 (washing)
Protein markers
EV: CD81/ Flotilin1
non-EV: Aconitase 2/ Cell organelle protein/ GAPDH/ Calreticulin/ VDAC1/ Beta-actin Proteomics
yes
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Pelleting: rotor type
70.1Ti
Pelleting: adjusted k-factor
101.8
Wash: volume per pellet (ml)
9
Wash: Rotor Type
70.1Ti
Wash: adjusted k-factor
101.8
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81/ Flotilin1
Detected contaminants
Cell organelle protein/ "VDAC1/ Calreticulin/ Aconitase 2/ GAPDH/ Beta-actin"
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140011 | 1/2 | Homo sapiens | BALF | (d)(U)C Filtration |
Rodríguez M | 2014 | 44% | |
Study summaryFull title
All authors
Rodríguez M, Silva J, López-Alfonso A, López-Muñiz MB, Peña C, Domínguez G, García JM, López-Gónzalez A, Méndez M, Provencio M, García V, Bonilla F
Journal
Genes Chromosomes Cancer
Abstract
Tumor-derived exosomes mediate tumorigenesis by facilitating tumor growth, metastasis, development o (show more...)
EV-METRIC
44% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
BALF
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C + Filtration
Protein markers
EV: CD63
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
BALF
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV140011 | 2/2 | Homo sapiens | Blood plasma | (d)(U)C Filtration |
Rodríguez M | 2014 | 44% | |
Study summaryFull title
All authors
Rodríguez M, Silva J, López-Alfonso A, López-Muñiz MB, Peña C, Domínguez G, García JM, López-Gónzalez A, Méndez M, Provencio M, García V, Bonilla F
Journal
Genes Chromosomes Cancer
Abstract
Tumor-derived exosomes mediate tumorigenesis by facilitating tumor growth, metastasis, development o (show more...)
EV-METRIC
44% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C + Filtration
Protein markers
EV: CD63
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV140092 | 1/2 | Homo sapiens | Cell culture supernatant | DG (d)(U)C |
Putz U | 2014 | 44% | |
Study summaryFull title
All authors
Putz U, Mah S, Goh CP, Low LH, Howitt J, Tan SS
Journal
Methods
Abstract
PTEN was discovered as a membrane-associated tumor suppressor protein nearly two decades ago, but th (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C
Protein markers
EV: Alix/ TSG101/ Flotillin
non-EV: Cell organelle protein Proteomics
no
EV density (g/ml)
1.140
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
70
Density gradient
Only used for validation of main results
1
Density medium
Sucrose
Lowest density fraction
0.25
Highest density fraction
2.5
Orientation
Bottom-up
Speed (g)
100000
Pelleting-wash: volume per pellet (mL)
10
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ TSG101/ Flotillin
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
Flotillin
Characterization: Particle analysis
EM
EM-type
immune EM
Proteïns
PTEN;Ndfip1
Image type
Close-up
|
||||||||
EV140092 | 2/2 | Homo sapiens | Cell culture supernatant | DG (d)(U)C |
Putz U | 2014 | 44% | |
Study summaryFull title
All authors
Putz U, Mah S, Goh CP, Low LH, Howitt J, Tan SS
Journal
Methods
Abstract
PTEN was discovered as a membrane-associated tumor suppressor protein nearly two decades ago, but th (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C
Protein markers
EV: Alix/ TSG101/ Flotillin
non-EV: Cell organelle protein Proteomics
no
EV density (g/ml)
1.140
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
70
Density gradient
Only used for validation of main results
1
Density medium
Sucrose
Lowest density fraction
0.25
Highest density fraction
2.5
Orientation
Bottom-up
Speed (g)
100000
Pelleting-wash: volume per pellet (mL)
10
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ TSG101/ Flotillin
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
Flotillin
Characterization: Particle analysis
EM
EM-type
immune EM
Proteïns
PTEN;Ndfip1
Image type
Close-up
|
||||||||
EV140131 | 2/3 | Homo sapiens | Platelet supernatant | DG (d)(U)C filter Filtration |
Pienimaeki-Roemer A | 2014 | 44% | |
Study summaryFull title
All authors
Pienimaeki-Roemer A, Kuhlmann K, Böttcher A, Konovalova T, Black A, Orsó E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G
Journal
Transfusion
Abstract
BACKGROUND: Platelets (PLTs) in stored PLT concentrates (PLCs) release PLT extracellular vesicles (P (show more...)
EV-METRIC
44% (80th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Platelet supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
DG + (d)(U)C + filter + Filtration
Adj. k-factor
784.6 (pelleting) / 784.6 (washing)
Protein markers
EV: Alix/ CD63/ CD9
non-EV: Proteomics
yes
EV density (g/ml)
1.12-1.15
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Platelet supernatant
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 10,000 g and 50,000 g
Obtain an EV pellet :
Yes
Pelleting: time(min)
40
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
784.6
Wash: Rotor Type
70Ti
Wash: adjusted k-factor
784.6
Density gradient
Density medium
Iodixanol
Lowest density fraction
10
Highest density fraction
30
Orientation
Top-down
Rotor type
SW41
Speed (g)
100000
Pelleting-wash: volume per pellet (mL)
2
Other
Name other separation method
filter
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ CD9
Characterization: Particle analysis
NTA
|
||||||||
EV140131 | 3/3 | Homo sapiens | Platelet supernatant | DG (d)(U)C filter Filtration |
Pienimaeki-Roemer A | 2014 | 44% | |
Study summaryFull title
All authors
Pienimaeki-Roemer A, Kuhlmann K, Böttcher A, Konovalova T, Black A, Orsó E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G
Journal
Transfusion
Abstract
BACKGROUND: Platelets (PLTs) in stored PLT concentrates (PLCs) release PLT extracellular vesicles (P (show more...)
EV-METRIC
44% (80th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Platelet supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
DG + (d)(U)C + filter + Filtration
Adj. k-factor
130.7 (pelleting) / 130.7 (washing)
Protein markers
EV: Alix/ CD63/ CD9
non-EV: Proteomics
yes
EV density (g/ml)
1.12-1.15
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Platelet supernatant
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
70
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
130.7
Wash: Rotor Type
70Ti
Wash: adjusted k-factor
130.7
Density gradient
Density medium
Iodixanol
Lowest density fraction
10
Highest density fraction
30
Orientation
Top-down
Rotor type
SW41
Speed (g)
100000
Pelleting-wash: volume per pellet (mL)
2
Other
Name other separation method
filter
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ CD9
Characterization: Particle analysis
NTA
|
||||||||
EV140038 | 1/1 | Homo sapiens | Cell culture supernatant | (d)(U)C | Park S | 2014 | 44% | |
Study summaryFull title
All authors
Park S, Ahn ES, Kim Y
Journal
Cell Biol Int
Abstract
The identification of small vesicles released by many cell types as tools of intercellular communica (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
156.9 (pelleting) / 126 (washing)
Protein markers
EV: HSP90/ Flotilin1/ MHC2
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
70
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
156.9
Wash: Rotor Type
90Ti
Wash: adjusted k-factor
126.0
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Flotilin1/ HSP90/ MHC2
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
MHC2
Characterization: Particle analysis
DLS
EM
EM-type
cryo EM
Image type
Wide-field
|
||||||||
EV140203 | 1/1 | Mus musculus | Cell culture supernatant | DG (d)(U)C Filtration |
Padro CJ | 2014 | 44% | |
Study summaryFull title
All authors
Padro CJ, Shawler TM, Gormley MG, Sanders VM
Journal
J Immunol
Abstract
Soluble CD23 plays a role in the positive regulation of an IgE response. Engagement of the ?2 adrene (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C + Filtration
Adj. k-factor
255.8 (pelleting)
Protein markers
EV: ADAM10/ CD63
non-EV: Proteomics
no
EV density (g/ml)
1.05-1.1
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
60
Pelleting: rotor type
SW41
Pelleting: adjusted k-factor
255.8
Density gradient
Only used for validation of main results
1
Density medium
Iodixanol
Lowest density fraction
1.00g/L
Highest density fraction
1.25g/L
Orientation
Top-down
Speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ ADAM10
ELISA
Detected EV-associated proteins
ADAM10
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV140088 | 1/1 | Homo sapiens | Cell culture supernatant | (d)(U)C Filtration |
Ohshima K | 2014 | 44% | |
Study summaryFull title
All authors
Ohshima K, Kanto K, Hatakeyama K, Ide T, Wakabayashi-Nakao K, Watanabe Y, Sakura N, Terashima M, Yamaguchi K, Mochizuki T
Journal
Proteomics
Abstract
Exosomes are small vesicles secreted from cells that transport their embedded molecules through bidi (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C + Filtration
Adj. k-factor
156.9 (pelleting) / 93.46 (washing)
Protein markers
EV: Tubulin/ Alix/ Beta-actin/ GAPDH/ Syntenin
non-EV: Proteomics
yes
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
156.9
Wash: volume per pellet (ml)
6
Wash: Rotor Type
100Ti
Wash: adjusted k-factor
93.46
Filtration steps
0.2µm > x > 0.1µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ Syntenin/ Beta-actin/ GAPDH/ Tubulin
ELISA
Detected EV-associated proteins
Beta-actin/ GAPDH/ Tubulin
Characterization: Particle analysis
|
||||||||
EV140119 | 2/3 | Homo sapiens | Cell culture supernatant | DG (d)(U)C Filtration |
Liu Z | 2014 | 44% | |
Study summaryFull title
All authors
Liu Z, Zhang X, Yu Q, He JJ
Journal
Biochem Biophys Res Commun
Abstract
Hepatitis C virus (HCV) infects its target cells in the form of cell-free viruses and through cell-c (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C + Filtration
Adj. k-factor
55.47 (pelleting)
Protein markers
EV: AChE/ CD63/ HSP70
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
SW55
Pelleting: adjusted k-factor
55.47
Density gradient
Only used for validation of main results
1
Density medium
Iodixanol
Lowest density fraction
6
Highest density fraction
24
Orientation
Top-down
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ HSP70/ AChE
ELISA
Detected EV-associated proteins
AChE
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV140119 | 3/3 | Homo sapiens | Cell culture supernatant | DG (d)(U)C Filtration |
Liu Z | 2014 | 44% | |
Study summaryFull title
All authors
Liu Z, Zhang X, Yu Q, He JJ
Journal
Biochem Biophys Res Commun
Abstract
Hepatitis C virus (HCV) infects its target cells in the form of cell-free viruses and through cell-c (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C + Filtration
Adj. k-factor
195.3 (pelleting)
Protein markers
EV: AChE/ CD63/ HSP70
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
SW28
Pelleting: adjusted k-factor
195.3
Density gradient
Only used for validation of main results
1
Density medium
Iodixanol
Lowest density fraction
6
Highest density fraction
24
Orientation
Top-down
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ HSP70/ AChE
ELISA
Detected EV-associated proteins
AChE
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV140031 | 1/2 | Homo sapiens | Urine | (d)(U)C | Kosanovi? M | 2014 | 44% | |
Study summaryFull title
All authors
Kosanovic M, Jankovic M
Journal
Biotechniques
Abstract
Urine is a readily available source of relatively large quantities of extracellular vesicles (EVs). (show more...)
EV-METRIC
44% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes / extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
157.1 (pelleting)
Protein markers
EV: CD63/ Galectin3
non-EV: Tamm-Horsfall glycoprotein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Pelleting: rotor type
50.2Ti
Pelleting: adjusted k-factor
157.1
Wash: volume per pellet (ml)
6
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ Galectin3
Detected contaminants
Tamm-Horsfall glycoprotein
ELISA
Detected EV-associated proteins
CD63/ Galectin3
Detected contaminants
Tamm-Horsfall glycoprotein
Characterization: Particle analysis
|
||||||||
EV140031 | 2/2 | Homo sapiens | Urine | (d)(U)C Filtration |
Kosanovi? M | 2014 | 44% | |
Study summaryFull title
All authors
Kosanovic M, Jankovic M
Journal
Biotechniques
Abstract
Urine is a readily available source of relatively large quantities of extracellular vesicles (EVs). (show more...)
EV-METRIC
44% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes / extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C + Filtration
Adj. k-factor
157.1 (pelleting)
Protein markers
EV: CD63/ Galectin3
non-EV: Tamm-Horsfall glycoprotein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Pelleting: rotor type
50.2Ti
Pelleting: adjusted k-factor
157.1
Wash: volume per pellet (ml)
6
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ Galectin3
Detected contaminants
Tamm-Horsfall glycoprotein
ELISA
Detected EV-associated proteins
CD63/ Galectin3
Detected contaminants
Tamm-Horsfall glycoprotein
Characterization: Particle analysis
EM
EM-type
transmission EM/ scanning EM
Image type
Wide-field
|
||||||||
EV140083 | 1/1 | Homo sapiens | Cell culture supernatant | DG (d)(U)C Filtration |
Koch R | 2014 | 44% | |
Study summaryFull title
All authors
Koch R, Demant M, Aung T, Diering N, Cicholas A, Chapuy B, Wenzel D, Lahmann M, Güntsch A, Kiecke C, Becker S, Hupfeld T, Venkataramani V, Ziepert M, Opitz L, Klapper W, Trümper L, Wulf GG
Journal
Blood
Abstract
Tumors are composed of phenotypically heterogeneous cell populations. The nongenomic mechanisms unde (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C + Filtration
Adj. k-factor
213.3 (pelleting)
Protein markers
EV: Flotillin2
non-EV: Proteomics
no
EV density (g/ml)
1.160
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
240
Pelleting: rotor type
32Ti
Pelleting: adjusted k-factor
213.3
Density gradient
Only used for validation of main results
1
Density medium
Sucrose
Lowest density fraction
0.25
Highest density fraction
2.25
Orientation
Top-down
Rotor type
SW32
Speed (g)
110000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Flotillin2
ELISA
Detected EV-associated proteins
Flotillin2
Characterization: Particle analysis
EM
EM-type
immune EM
Proteïns
Wnt3a
Image type
Close-up, Wide-field
|
||||||||
EV140179 | 1/1 | Homo sapiens | Cell culture supernatant | DG (d)(U)C |
Klinker MW | 2014 | 44% | |
Study summaryFull title
All authors
Klinker MW, Lizzio V, Reed TJ, Fox DA, Lundy SK
Journal
Front Immunol
Abstract
Immune suppression mediated by exosomes is an emerging concept with potentially immense utility for (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C
Protein markers
EV: Beta-actin/ MHC2/ FasL
non-EV: FasL Proteomics
no
EV density (g/ml)
1.160
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
60-240
Density gradient
Only used for validation of main results
1
Density medium
Iodixanol
Lowest density fraction
1.03g/l
Highest density fraction
1.27g/l
Orientation
Top-down
Speed (g)
100000
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
MHC2/ FasL/ MHC2/ Beta-actin
Detected contaminants
FasL
ELISA
Detected EV-associated proteins
MHC2/ FasL/ MHC2/ Beta-actin
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
Particle analysis: flow cytometry
|
||||||||
EV140008 | 1/2 | Homo sapiens | Cell culture supernatant | (d)(U)C Filtration |
Kahlert C | 2014 | 44% | |
Study summaryFull title
All authors
Kahlert C, Melo SA, Protopopov A, Tang J, Seth S, Koch M, Zhang J, Weitz J, Chin L, Futreal A, Kalluri R
Journal
J Biol Chem
Abstract
Exosomes are small vesicles (50-150 nm) of endocytic origin that are released by many different cell (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C + Filtration
Protein markers
EV: TSG101/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Wash: volume per pellet (ml)
30
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD9/ TSG101
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140008 | 2/2 | Homo sapiens | Serum | (d)(U)C Filtration |
Kahlert C | 2014 | 44% | |
Study summaryFull title
All authors
Kahlert C, Melo SA, Protopopov A, Tang J, Seth S, Koch M, Zhang J, Weitz J, Chin L, Futreal A, Kalluri R
Journal
J Biol Chem
Abstract
Exosomes are small vesicles (50-150 nm) of endocytic origin that are released by many different cell (show more...)
EV-METRIC
44% (88th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C + Filtration
Protein markers
EV: TSG101/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
Differential ultracentrifugation
centrifugation steps
Equal to or above 150,000 g
Obtain an EV pellet :
Yes
Pelleting: time(min)
960
Wash: volume per pellet (ml)
11
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD9/ TSG101
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140007 | 1/1 | Mus musculus | Cell culture supernatant | DG (d)(U)C |
Ju R | 2014 | 44% | |
Study summaryFull title
All authors
Ju R, Zhuang ZW, Zhang J, Lanahan AA, Kyriakides T, Sessa WC, Simons M
Journal
J Biol Chem
Abstract
Angiopoietin-2 (Ang2) is an extracellular protein and one of the principal ligands of Tie2 receptor (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
Separation protocol
Separation protocol
DG + (d)(U)C
Protein markers
EV: HSP90/ CD63/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Density gradient
Only used for validation of main results
1
Density medium
Iodixanol
Lowest density fraction
5
Highest density fraction
40
Orientation
Bottom-up
Speed (g)
100000
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ HSP90/ Syntenin
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140030 | 2/2 | Mus musculus | Cell culture supernatant | (d)(U)C Microfluidics |
Jo W | 2014 | 44% | |
Study summaryFull title
All authors
Jo W, Jeong D, Kim J, Cho S, Jang SC, Han C, Kang JY, Gho YS, Park J
Journal
Lab Chip
Abstract
Exosomes/microvesicles are known to shuttle biological signals between cells, possibly by transferri (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
Nano(-sized) vesicles
Separation protocol
Separation protocol
(d)(U)C + Microfluidics
Protein markers
EV: Nanog/ Actin/ ICAM1
non-EV: Proteomics
no
Show all info
Study aim
Other/Generation of nanovesicles
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Other
Name other separation method
Microfluidics
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Actin/ Nanog/ ICAM1
ELISA
Detected EV-associated proteins
Actin/ Nanog/ ICAM1
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140006 | 1/1 | Homo sapiens | Cell culture supernatant | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Nawrocki A, Jensen SG, Thorsen K, Whitehead B, Howard KA, Dyrskjøt L, Ørntoft TF, Larsen MR, Ostenfeld MS
Journal
Proteomics
Abstract
Cancer cells secrete soluble factors and various extracellular vesicles, including exosomes, into th (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD63/ CD81/ GAPDH/ Alix/ Syntenin/ Beta-actin/ CD9
non-EV: Cell organelle protein Proteomics
yes
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ CD81/ CD9/ Syntenin/ Beta-actin/ GAPDH
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
Beta-actin/ GAPDH
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140029 | 1/12 | Homo sapiens | Cell culture supernatant | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
475.5 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
475.5
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 2/12 | Homo sapiens | Cell culture supernatant | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
234.2 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
234.2
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 3/12 | Homo sapiens | Cell culture supernatant | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
156.9 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
156.9
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 4/12 | Homo sapiens | Cell culture supernatant | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
117.9 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
117.9
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 5/12 | Homo sapiens | Cell culture supernatant | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
93.96 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Equal to or above 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
93.96
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 6/12 | Homo sapiens | Cell culture supernatant | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
78.45 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Equal to or above 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
78.45
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 7/12 | Homo sapiens | Cell culture supernatant | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
475.5 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
475.5
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 8/12 | Homo sapiens | Cell culture supernatant | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
234.2 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
234.2
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 9/12 | Homo sapiens | Cell culture supernatant | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
156.9 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Between 50,000 g and 100,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
156.9
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 10/12 | Homo sapiens | Cell culture supernatant | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
117.9 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Between 50,000 g and 100,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
117.9
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 11/12 | Homo sapiens | Cell culture supernatant | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
93.96 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Equal to or above 150,000 g Between 50,000 g and 100,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
93.96
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140029 | 12/12 | Homo sapiens | Cell culture supernatant | (d)(U)C | Jeppesen DK | 2014 | 44% | |
Study summaryFull title
All authors
Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA, Ostenfeld MS
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ i (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
78.45 (pelleting)
Protein markers
EV: CD81/ TSG101/ Syntenin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Differential ultracentrifugation
centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Equal to or above 150,000 g Between 50,000 g and 100,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
78.45
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81/ Syntenin/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
|
||||||||
EV140027 | 1/1 | Homo sapiens | Cell culture supernatant | (d)(U)C | Inder KL | 2014 | 44% | |
Study summaryFull title
All authors
Inder KL, Ruelcke JE, Petelin L, Moon H, Choi E, Rae J, Blumenthal A, Hutmacher D, Saunders NA, Stow JL, Parton RG, Hill MM
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Tumour-derived extracellular vesicles (EVs) play a role in tumour progression; however, (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Cell Name
DNF
Sample origin
DNF
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD63/ CD98/ EphA2/ Cofilin/ Caveolin1/ 4F2
non-EV: Cell organelle protein Proteomics
no
TEM measurements
86.9+-4.7
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ Caveolin1/ Cofilin/ EphA2/ 4F2/ CD98
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
Caveolin1/ Cofilin/ EphA2/ 4F2/ CD98
Characterization: Particle analysis
EM
EM-type
transmission EM/ immune EM
Proteïns
4F2
Image type
Close-up, Wide-field
|
||||||||
EV140123 | 2/2 | Homo sapiens | Blood plasma | DG (d)(U)C Filtration |
Hong CS | 2014 | 44% | |
Study summaryFull title
All authors
Hong CS, Muller L, Whiteside TL, Boyiadzis M
Journal
Front Immunol
Abstract
PURPOSE: Exosomes isolated from the plasma of newly diagnosed acute myeloid leukemia (AML) patients (show more...)
EV-METRIC
44% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C + Filtration
Protein markers
EV: CD81
non-EV: Proteomics
no
EV density (g/ml)
1.16-1.19
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Density gradient
Density medium
Sucrose
Lowest density fraction
0.25
Highest density fraction
2.5
Orientation
Top-down
Speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
NTA
Particle analysis: flow cytometry
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV140117 | 2/8 | Homo sapiens | Blood plasma | (d)(U)C | He M | 2014 | 44% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
44% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Cell Name
DNF
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD81/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
120
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD81/ CD9
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140026 | 1/1 | Mus musculus | Cell culture supernatant | DG (d)(U)C Filtration |
Han KY | 2014 | 44% | |
Study summaryFull title
All authors
Han KY, Dugas-Ford J, Seiki M, Chang JH, Azar DT
Journal
Invest Ophthalmol Vis Sci
Abstract
PURPOSE: Matrix metalloproteinase (MMP) 14 has been shown to promote angiogenesis, but the underlyin (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
|