Search > Results
You searched for: EV210036 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210036 | 1/2 | Homo sapiens | HT-29 |
(d)(U)C UF |
Knol, Jaco C | 2016 | 29% | |
Study summaryFull title
All authors
Jaco C Knol, Inge de Reus, Tim Schelfhorst, Robin Beekhof, Meike de Wit, Sander R Piersma, Thang V Pham, Egbert F Smit, Henk M W Verheul, Connie R Jiménez
Journal
EuPA Open Proteom.
Abstract
Extracellular vesicles (EVs) are cell-secreted membrane vesicles enclosed by a lipid bilayer derived (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Ultrafiltration Protein markers
EV: None
non-EV: None Proteomics
yes
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HT-29
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
SW 40 Ti
Pelleting: speed (g)
114000
Wash: volume per pellet (ml)
13.2
Wash: time (min)
90
Wash: Rotor Type
SW 40 Ti
Wash: speed (g)
114000
Ultra filtration
Cut-off size (kDa)
3
Membrane type
Not specified
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
No
Characterization: Lipid analysis
No
|
||||||||
EV210036 | 2/2 | Homo sapiens | HT-29 |
UF ME kit (New England Peptide) |
Knol, Jaco C | 2016 | 17% | |
Study summaryFull title
All authors
Jaco C Knol, Inge de Reus, Tim Schelfhorst, Robin Beekhof, Meike de Wit, Sander R Piersma, Thang V Pham, Egbert F Smit, Henk M W Verheul, Connie R Jiménez
Journal
EuPA Open Proteom.
Abstract
Extracellular vesicles (EVs) are cell-secreted membrane vesicles enclosed by a lipid bilayer derived (show more...)
EV-METRIC
17% (54th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Ultrafiltration
Commercial method Protein markers
EV: CD81/ HSP70/ CD63
non-EV: None Proteomics
yes
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HT-29
EV-harvesting Medium
Serum free medium
Separation Method
Ultra filtration
Cut-off size (kDa)
3
Membrane type
Not specified
Commercial kit
ME kit (New England Peptide)
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ HSP70/ CD81
Proteomics database
No
Characterization: Lipid analysis
No
|
||||||||
1 - 2 of 2 |
EV-TRACK ID | EV210036 | |
---|---|---|
species | Homo sapiens | |
sample type | Cell culture | |
cell type | HT-29 | |
condition | Control condition | |
separation protocol | dUC Ultrafiltration | Ultrafiltration ME kit (New England Peptide) |
Exp. nr. | 1 | 2 |
EV-METRIC % | 29 | 17 |