EV-TRACK

Search > Results

You searched for: EV210032 (EV-TRACK ID)

Showing 1 - 1 of 1

Results list Study Tree

Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV210032	1/2	Homo sapiens	Blood plasma	(d)(U)C	Linares, Romain	2015	29%
Study summary Full title High-speed centrifugation induces aggregation of extracellular vesicles All authors Romain Linares, Sisareuth Tan, Céline Gounou, Nicolas Arraud, Alain R Brisson Journal J Extracell Vesicles Abstract Plasma and other body fluids contain cell-derived extracellular vesicles (EVs), which participate in (show more...)Plasma and other body fluids contain cell-derived extracellular vesicles (EVs), which participate in physiopathological processes and have potential biomedical applications. In order to isolate, concentrate and purify EVs, high-speed centrifugation is often used. We show here, using electron microscopy, receptor-specific gold labelling and flow cytometry, that high-speed centrifugation induces the formation of EV aggregates composed of a mixture of EVs of various phenotypes and morphologies. The presence of aggregates made of EVs of different phenotypes may lead to erroneous interpretation concerning the existence of EVs harbouring surface antigens from different cell origins. (hide) EV-METRIC 29% (61st percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Blood plasma Sample origin Control condition Focus vesicles extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (Differential) (ultra)centrifugation Protein markers EV: Annexin A5/ CD235a/ CD41 non-EV: None Proteomics no Show all info Study aim Technical analysis comparing/optimizing EV-related methods Sample Species Homo sapiens Sample Type Blood plasma Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 800 g and 10,000 g Pelleting performed No Characterization: Protein analysis Protein Concentration Method Not determined Flow cytometry Type of Flow cytometry Gallios (Beckman Coulter) Calibration bead size 0.4 Antibody details provided? No Detected EV-associated proteins Annexin A5/ CD235a/ CD41 Characterization: Lipid analysis No Characterization: Particle analysis EM EM-type Immuno-EM/ Cryo-EM EM protein Annexin A5; CD235a; CD41 Image type Close-up, Wide-field EV concentration Yes

				1 - 1 of 1

EV-TRACK ID	EV210032
species	Homo sapiens
sample type	Blood plasma
condition	Control condition
separation protocol	dUC
Exp. nr.	1
EV-METRIC %	29