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You searched for: EV210027 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210027 | 1/2 | Homo sapiens | Primary adipose-derived stem cells |
(d)(U)C Filtration |
Han, Yu-di | 2018 | 34% | |
Study summaryFull title
All authors
Yu-di Han, Yun Bai, Xin-Long Yan, Jing Ren, Quan Zeng, Xiao-Dong Li, Xue-Tao Pei, Yan Han
Journal
Biochem Biophys Res Commun
Abstract
Background: Adipose-derived stromal cells (ADSCs)-derived exosomes (ADSC-Exos) account for the proan (show more...)
EV-METRIC
34% (78th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: TSG101/ CD63/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Primary adipose-derived stem cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
not specified
Wash: time (min)
60
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
110000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ TSG101
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Detected EV-associated proteins
CD9/ CD63/ TSG101
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
98
EV concentration
Yes
Particle yield
No NA
EM
EM-type
Transmission electron microscopy
Image type
Wide-field
|
||||||||
EV210027 | 2/2 | Homo sapiens | Primary adipose-derived stem cells |
(d)(U)C Filtration |
Han, Yu-di | 2018 | 15% | |
Study summaryFull title
All authors
Yu-di Han, Yun Bai, Xin-Long Yan, Jing Ren, Quan Zeng, Xiao-Dong Li, Xue-Tao Pei, Yan Han
Journal
Biochem Biophys Res Commun
Abstract
Background: Adipose-derived stromal cells (ADSCs)-derived exosomes (ADSC-Exos) account for the proan (show more...)
EV-METRIC
15% (53rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Hypoxia
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: TSG101/ CD63/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Primary adipose-derived stem cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
not specified
Wash: time (min)
60
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
110000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
87
EV concentration
Yes
EM
EM-type
Transmission electron microscopy
Image type
Wide-field
|
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1 - 2 of 2 |
EV-TRACK ID | EV210027 | |
---|---|---|
species | Homo sapiens | |
sample type | Cell culture | |
cell type | Primary adipose-derived stem cells | |
condition | Control condition | Hypoxia |
separation protocol | dUC Filtration | dUC Filtration |
Exp. nr. | 1 | 2 |
EV-METRIC % | 34 | 15 |