EV-TRACK

Search > Results

You searched for: EV210014 (EV-TRACK ID)

Showing 1 - 2 of 2

Results list Study Tree

Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV210014	1/2	Homo sapiens	HMEC-1	dUC	Ceroi, Adam	2016	13%
Study summary Full title The anti-inflammatory effects of platelet-derived microparticles in human plasmacytoid dendritic cells involve liver X receptor activation All authors Adam Ceroi, Fanny Angelot Delettre, Charline Marotel, Thierry Gauthier, Afag Asgarova, Sabéha Biichlé, Anne Duperrier, Guillaume Mourey, Sylvain Perruche, Laurent Lagrost, David Masson, Philippe Saas Journal Haematologica Abstract NA (show more...)NA (hide) EV-METRIC 13% (34th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Cell culture supernatant Sample origin Control condition Focus vesicles microparticle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture dUC Protein markers EV: None non-EV: None Proteomics no Show all info Study aim Function/Mechanism of uptake/transfer Sample Species Homo sapiens Sample Type Cell culture supernatant EV-producing cells HMEC-1 EV-harvesting Medium Serum-containing medium Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed Yes Pelleting: time(min) 90 Pelleting: rotor type Not specified Pelleting: speed (g) 15000 Other Name other separation method dUC Characterization: Protein analysis Protein Concentration Method Not determined Flow cytometry Type of Flow cytometry Navios (Beckman Coulter) Calibration bead size 6 Antibody details provided? No Detected EV-associated proteins CD41/ CD31/ CD235 Characterization: Lipid analysis Yes Characterization: Particle analysis Particle analysis: flow cytometry Flow cytometer type Navios (Beckman Coulter) Calibration bead size 6 Report type Not Reported EV concentration Yes

	EV210014	2/2	Homo sapiens	platelet concentrate	dUC	Ceroi, Adam	2016	13%
Study summary Full title The anti-inflammatory effects of platelet-derived microparticles in human plasmacytoid dendritic cells involve liver X receptor activation All authors Adam Ceroi, Fanny Angelot Delettre, Charline Marotel, Thierry Gauthier, Afag Asgarova, Sabéha Biichlé, Anne Duperrier, Guillaume Mourey, Sylvain Perruche, Laurent Lagrost, David Masson, Philippe Saas Journal Haematologica Abstract NA (show more...)NA (hide) EV-METRIC 13% (50th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type platelet concentrate Sample origin Control condition Focus vesicles microparticle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture dUC Protein markers EV: None non-EV: None Proteomics no Show all info Study aim Function/Mechanism of uptake/transfer Sample Species Homo sapiens Sample Type platelet concentrate Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed Yes Pelleting: time(min) 90 Pelleting: rotor type Not specified Pelleting: speed (g) 15000 Other Name other separation method dUC Characterization: Protein analysis Protein Concentration Method Not determined Flow cytometry Type of Flow cytometry Navios (Beckman Coulter) Calibration bead size 6 Antibody details provided? No Detected EV-associated proteins CD41/ CD31/ CD235 Characterization: Lipid analysis Yes Characterization: Particle analysis Particle analysis: flow cytometry Flow cytometer type Navios (Beckman Coulter) Calibration bead size 6 Report type Not Reported EV concentration Yes

				1 - 2 of 2

EV-TRACK ID	EV210014
species	Homo sapiens
sample type	Cell culture	platelet concentrate
cell type	HMEC-1	NA
medium	Serum-containing medium	NA
condition	Control condition	Control condition
separation protocol	dUC	dUC
Exp. nr.	1	2
EV-METRIC %	13	13