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You searched for: EV200192 (EV-TRACK ID)
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Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200192 | 1/2 | Exophiala dermatitidis | EXF-10123 |
(d)(U)C Filtration UF DG |
Lavrin, Teja | 2020 | 58% | |
Study summaryFull title
All authors
Teja Lavrin, Tilen Konte, Rok Kostanjšek, Simona Sitar, Kristina Sepčič, Sonja Prpar Mihevc, Ema Žagar, Vera Župunski, Metka Lenassi, Boris Rogelj, Nina Gunde Cimerman
Journal
Cells
Abstract
The neurotropic and extremophilic black yeast Exophiala dermatitidis (Herpotrichellaceae) inhabits d (show more...)
EV-METRIC
58% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Ultrafiltration Density gradient Protein markers
EV: None
non-EV: None Proteomics
no
EV density (g/ml)
1.09–1.32
Show all info
Study aim
Function
Sample
Species
Exophiala dermatitidis
Sample Type
Cell culture supernatant
EV-producing cells
EXF-10123
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
MLA-50
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
70
Wash: Rotor Type
TLA-55
Wash: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
20
Highest density fraction
60
Total gradient volume, incl. sample (mL)
4.8
Sample volume (mL)
0.4
Orientation
Not specified
Rotor type
Not specified
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
0.4
Fraction processing
Precipitation
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Not specified
Other
Name other separation method
Filtration
Other
Name other separation method
Ultrafiltration
Other
Name other separation method
Density gradient
Characterization: Protein analysis
None
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Hardware adjustment
EM
EM-type
Transmission-EM
Image type
Close-up
Report type
Mean
Report size
90
EV-concentration
Yes
|
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EV200192 | 2/2 | Exophiala dermatitidis | EXF-10123 |
(d)(U)C Filtration UF DG |
Lavrin, Teja | 2020 | 58% | |
Study summaryFull title
All authors
Teja Lavrin, Tilen Konte, Rok Kostanjšek, Simona Sitar, Kristina Sepčič, Sonja Prpar Mihevc, Ema Žagar, Vera Župunski, Metka Lenassi, Boris Rogelj, Nina Gunde Cimerman
Journal
Cells
Abstract
The neurotropic and extremophilic black yeast Exophiala dermatitidis (Herpotrichellaceae) inhabits d (show more...)
EV-METRIC
58% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Melanin inhibitor
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Ultrafiltration Density gradient Protein markers
EV: None
non-EV: None Proteomics
no
EV density (g/ml)
1.09–1.32
Show all info
Study aim
Function
Sample
Species
Exophiala dermatitidis
Sample Type
Cell culture supernatant
EV-producing cells
EXF-10123
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
MLA-50
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
70
Wash: Rotor Type
TLA-55
Wash: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
20
Highest density fraction
60
Total gradient volume, incl. sample (mL)
4.8
Sample volume (mL)
0.4
Orientation
Not specified
Rotor type
Not specified
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
0.4
Fraction processing
Precipitation
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Not specified
Other
Name other separation method
Filtration
Other
Name other separation method
Ultrafiltration
Other
Name other separation method
Density gradient
Characterization: Protein analysis
None
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Hardware adjustment
EM
EM-type
Transmission-EM
Image type
Close-up
Report type
Mean
Report size
75
EV-concentration
Yes
|
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1 - 2 of 2 |
EV-TRACK ID | EV200192 | |
---|---|---|
species | Exophiala dermatitidis | |
sample type | Cell culture | |
cell type | EXF-10123 | |
condition | Control condition | Melanin inhibitor |
separation protocol | dUC Filtration Ultrafiltration Density gradient | dUC Filtration Ultrafiltration Density gradient |
Exp. nr. | 1 | 2 |
EV-METRIC % | 58 | 58 |