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You searched for: EV200150 (EV-TRACK ID)
Showing 1 - 6 of 6
Showing 1 - 6 of 6
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200150 | 1/6 | Homo sapiens | Blood plasma | (d)(U)C | Sandrim, V C | 2016 | 0% | |
Study summaryFull title
All authors
V C Sandrim, M R Luizon, A C Palei, J E Tanus-Santos, R C Cavalli
Journal
BJOG: An International Journal of Obstetrics and Gynaecology
Abstract
Objective: To validate and to compare the circulating microRNA (miR) expression profiles between pre (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Healthy pregnant
Focus vesicles
apoptotic body
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
Yes
Pelleting: time(min)
20
Pelleting: rotor type
Not specified
Pelleting: speed (g)
2000
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
|
||||||||
EV200150 | 2/6 | Homo sapiens | Blood plasma | (d)(U)C | Sandrim, V C | 2016 | 0% | |
Study summaryFull title
All authors
V C Sandrim, M R Luizon, A C Palei, J E Tanus-Santos, R C Cavalli
Journal
BJOG: An International Journal of Obstetrics and Gynaecology
Abstract
Objective: To validate and to compare the circulating microRNA (miR) expression profiles between pre (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Healthy pregnant
Focus vesicles
microvesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
20
Pelleting: rotor type
Not specified
Pelleting: speed (g)
16500
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
|
||||||||
EV200150 | 3/6 | Homo sapiens | Blood plasma |
(d)(U)C Filtration |
Sandrim, V C | 2016 | 0% | |
Study summaryFull title
All authors
V C Sandrim, M R Luizon, A C Palei, J E Tanus-Santos, R C Cavalli
Journal
BJOG: An International Journal of Obstetrics and Gynaecology
Abstract
Objective: To validate and to compare the circulating microRNA (miR) expression profiles between pre (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Healthy pregnant
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
20
Pelleting: rotor type
Not specified
Pelleting: speed (g)
16500
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
|
||||||||
EV200150 | 4/6 | Homo sapiens | Blood plasma | (d)(U)C | Sandrim, V C | 2016 | 0% | |
Study summaryFull title
All authors
V C Sandrim, M R Luizon, A C Palei, J E Tanus-Santos, R C Cavalli
Journal
BJOG: An International Journal of Obstetrics and Gynaecology
Abstract
Objective: To validate and to compare the circulating microRNA (miR) expression profiles between pre (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Pre-eclampsia
Focus vesicles
apoptotic body
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
Yes
Pelleting: time(min)
20
Pelleting: rotor type
Not specified
Pelleting: speed (g)
2000
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
|
||||||||
EV200150 | 5/6 | Homo sapiens | Blood plasma | (d)(U)C | Sandrim, V C | 2016 | 0% | |
Study summaryFull title
All authors
V C Sandrim, M R Luizon, A C Palei, J E Tanus-Santos, R C Cavalli
Journal
BJOG: An International Journal of Obstetrics and Gynaecology
Abstract
Objective: To validate and to compare the circulating microRNA (miR) expression profiles between pre (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Pre-eclampsia
Focus vesicles
microvesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
20
Pelleting: rotor type
Not specified
Pelleting: speed (g)
16500
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
|
||||||||
EV200150 | 6/6 | Homo sapiens | Blood plasma |
(d)(U)C Filtration |
Sandrim, V C | 2016 | 0% | |
Study summaryFull title
All authors
V C Sandrim, M R Luizon, A C Palei, J E Tanus-Santos, R C Cavalli
Journal
BJOG: An International Journal of Obstetrics and Gynaecology
Abstract
Objective: To validate and to compare the circulating microRNA (miR) expression profiles between pre (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Pre-eclampsia
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
20
Pelleting: rotor type
Not specified
Pelleting: speed (g)
16500
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
|
||||||||
1 - 6 of 6 |
EV-TRACK ID | EV200150 | |||||
---|---|---|---|---|---|---|
species | Homo sapiens | |||||
sample type | Blood plasma | |||||
condition | Healthy pregnant | Healthy pregnant | Healthy pregnant | Pre-eclampsia | Pre-eclampsia | Pre-eclampsia |
separation protocol | dUC | dUC | dUC Filtration | dUC | dUC | dUC Filtration |
vesicle related term | apoptotic body | microvesicle | exosome | apoptotic body | microvesicle | exosome |
Exp. nr. | 1 | 2 | 3 | 4 | 5 | 6 |
EV-METRIC % | 0 | 0 | 0 | 0 | 0 | 0 |