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You searched for: EV190099 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV190099 | 1/3 | Mus musculus | Serum |
Filtration qEV |
Anastasi, Federica | 2020 | 50% | |
Study summaryFull title
All authors
Federica Anastasi, Francesco Greco, Marialaura Dilillo, Eleonora Vannini, Valentina Cappello, Laura Baroncelli, Mario Costa, Mauro Gemmi, Matteo Caleo & Liam A. McDonnell
Journal
Sci Rep
Abstract
Longitudinal analysis of disease models enables the molecular changes due to disease progression or (show more...)
EV-METRIC
50% (89th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Control condition
Focus vesicles
small extracellular vesicles
Separation protocol
Separation protocol
Filtration
qEV Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
New methodological development/Biomarker/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Serum
Separation Method
Filtration steps
0.22µm or 0.2µm
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
microBCA
Proteomics database
No
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
45
|
||||||||
EV190099 | 2/3 | Mus musculus | Serum |
Filtration Total Exosome Isolation |
Anastasi, Federica | 2020 | 50% | |
Study summaryFull title
All authors
Federica Anastasi, Francesco Greco, Marialaura Dilillo, Eleonora Vannini, Valentina Cappello, Laura Baroncelli, Mario Costa, Mauro Gemmi, Matteo Caleo & Liam A. McDonnell
Journal
Sci Rep
Abstract
Longitudinal analysis of disease models enables the molecular changes due to disease progression or (show more...)
EV-METRIC
50% (89th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Control condition
Focus vesicles
small extracellular vesicles
Separation protocol
Separation protocol
Filtration
Total Exosome Isolation Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
New methodological development/Biomarker/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Serum
Separation Method
Filtration steps
0.22µm or 0.2µm
Commercial kit
Total Exosome Isolation
Other
Name other separation method
Total Exosome Isolation
Characterization: Protein analysis
Protein Concentration Method
microBCA
Proteomics database
No
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
37
|
||||||||
EV190099 | 3/3 | Mus musculus | Serum |
Filtration qEV |
Anastasi, Federica | 2020 | 17% | |
Study summaryFull title
All authors
Federica Anastasi, Francesco Greco, Marialaura Dilillo, Eleonora Vannini, Valentina Cappello, Laura Baroncelli, Mario Costa, Mauro Gemmi, Matteo Caleo & Liam A. McDonnell
Journal
Sci Rep
Abstract
Longitudinal analysis of disease models enables the molecular changes due to disease progression or (show more...)
EV-METRIC
17% (62nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Glioblastoma multiforme
Focus vesicles
small extracellular vesicles
Separation protocol
Separation protocol
Filtration
qEV Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
New methodological development/Biomarker/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Serum
Separation Method
Filtration steps
0.22µm or 0.2µm
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
microBCA
Proteomics database
No
Characterization: Lipid analysis
No
|
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1 - 3 of 3 |
EV-TRACK ID | EV190099 | ||
---|---|---|---|
species | Mus musculus | ||
sample type | Serum | ||
condition | Control condition | Control condition | Glioblastoma multiforme |
separation protocol | Filtration qEV | Filtration Total Exosome Isolation | Filtration qEV |
Exp. nr. | 1 | 2 | 3 |
EV-METRIC % | 50 | 50 | 17 |