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You searched for: EV190077 (EV-TRACK ID)
Showing 1 - 4 of 4
Showing 1 - 4 of 4
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV190077 | 1/4 | Bos taurus | Milk |
DG (d)(U)C milk acidification casein removal SEC |
Andrea Ridolfi | 2020 | 75% | |
Study summaryFull title
All authors
Andrea Ridolfi, Marco Brucale, Costanza Montis, Lucrezia Caselli, Lucia Paolini, Anne Borup, Anders T Boysen, Francesca Loria, Martijn J C van Herwijnen, Marije Kleinjan, Peter Nejsum, Natasa Zarovni, Marca H M Wauben, Debora Berti, Paolo Bergese, Francesco Valle
Journal
Anal Chem
Abstract
The mechanical properties of extracellular vesicles (EVs) are known to influence their biological fu (show more...)
EV-METRIC
75% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Milk
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C milk acidification casein removal SEC Protein markers
EV: TSG101/ CD63/ Flotillin1/ CD9/ MFGE8
non-EV: beta-lactoglobulin/ Casein Proteomics
no
EV density (g/ml)
1.06-1.19
Show all info
Study aim
Other/Biophysical characterization
Sample
Species
Bos taurus
Sample Type
Milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
No
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
16
Lowest density fraction
10%
Highest density fraction
60%
Total gradient volume, incl. sample (mL)
12.45
Sample volume (mL)
6.5
Orientation
Top-down
Rotor type
SW 40 Ti
Speed (g)
197000
Duration (min)
960
Fraction volume (mL)
0.5
Fraction processing
Size-exclusion chromatography
Size-exclusion chromatography
Total column volume (mL)
15
Sample volume/column (mL)
2
Resin type
Sephadex G-100
Other
Name other separation method
milk acidification
Other
Name other separation method
casein removal
Characterization: Protein analysis
Protein Concentration Method
Other;Colorimetric Nanoplasmonic Assay
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Flotillin1/ CD9/ CD63/ MFGE8/ TSG101
Detected contaminants
beta-lactoglobulin
Not detected contaminants
Casein
Characterization: Lipid analysis
No
EM
EM-type
Atomic force-EM
Image type
Wide-field
Report size (nm)
40-160
|
||||||||
EV190077 | 3/4 | Ascaris suum | Ascaris suum supernatant |
(d)(U)C UF |
Andrea Ridolfi | 2020 | 29% | |
Study summaryFull title
All authors
Andrea Ridolfi, Marco Brucale, Costanza Montis, Lucrezia Caselli, Lucia Paolini, Anne Borup, Anders T Boysen, Francesca Loria, Martijn J C van Herwijnen, Marije Kleinjan, Peter Nejsum, Natasa Zarovni, Marca H M Wauben, Debora Berti, Paolo Bergese, Francesco Valle
Journal
Anal Chem
Abstract
The mechanical properties of extracellular vesicles (EVs) are known to influence their biological fu (show more...)
EV-METRIC
29% (83rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Ascaris suum supernatant
Sample origin
Mycoplasma-contaminated
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
UF Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Other/Biophysical characterization
Sample
Species
Ascaris suum
Sample Type
Ascaris suum supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 50
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
9
Wash: time (min)
70
Wash: Rotor Type
Type 50
Wash: speed (g)
110000
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Regenerated cellulose
Protein Concentration Method
Other;Colorimetric Nanoplasmonic Assay
Other 1
There are no specific Ascaris suum EV protein markers identified yet;Kan je dit hier verwijderen en in comments plaatsen? En comments ook toevoegen aan de print?
Detected EV-associated proteins
Not detected EV-associated proteins
Detected contaminants
Not detected contaminants
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
166
EM
EM-type
Atomic force-EM
Image type
Wide-field
Report size (nm)
60-240
|
||||||||
EV190077 | 2/4 | Ascaris suum | Ascaris suum supernatant |
(d)(U)C qEV UF |
Andrea Ridolfi | 2020 | 17% | |
Study summaryFull title
All authors
Andrea Ridolfi, Marco Brucale, Costanza Montis, Lucrezia Caselli, Lucia Paolini, Anne Borup, Anders T Boysen, Francesca Loria, Martijn J C van Herwijnen, Marije Kleinjan, Peter Nejsum, Natasa Zarovni, Marca H M Wauben, Debora Berti, Paolo Bergese, Francesco Valle
Journal
Anal Chem
Abstract
The mechanical properties of extracellular vesicles (EVs) are known to influence their biological fu (show more...)
EV-METRIC
17% (33rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Ascaris suum supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
qEV UF Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Other/Biophysical characterization
Sample
Species
Ascaris suum
Sample Type
Ascaris suum supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Regenerated cellulose
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
None
Protein Concentration Method
Other;Colorimetric Nanoplasmonic Assay
Other 1
There are no specific Ascaris suum EV protein markers identified yet;Kan je dit hier verwijderen en in comments plaatsen? En comments ook toevoegen aan de print?
Detected EV-associated proteins
Not detected EV-associated proteins
Detected contaminants
Not detected contaminants
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
165
EM
EM-type
Atomic force-EM
Image type
Wide-field
Report size (nm)
60-240
|
||||||||
EV190077 | 4/4 | Ascaris suum | Ascaris suum supernatant |
(d)(U)C qEV UF |
Andrea Ridolfi | 2020 | 17% | |
Study summaryFull title
All authors
Andrea Ridolfi, Marco Brucale, Costanza Montis, Lucrezia Caselli, Lucia Paolini, Anne Borup, Anders T Boysen, Francesca Loria, Martijn J C van Herwijnen, Marije Kleinjan, Peter Nejsum, Natasa Zarovni, Marca H M Wauben, Debora Berti, Paolo Bergese, Francesco Valle
Journal
Anal Chem
Abstract
The mechanical properties of extracellular vesicles (EVs) are known to influence their biological fu (show more...)
EV-METRIC
17% (33rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Ascaris suum supernatant
Sample origin
Mycoplasma-contaminated
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
qEV UF Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Other/Biophysical characterization
Sample
Species
Ascaris suum
Sample Type
Ascaris suum supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Regenerated cellulose
Commercial kit
qEV
Other
Name other separation method
qEV
Protein Concentration Method
Other;Colorimetric Nanoplasmonic Assay
Other 1
There are no specific Ascaris suum EV protein markers identified yet
Detected EV-associated proteins
Not detected EV-associated proteins
Detected contaminants
Not detected contaminants
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
157
EM
EM-type
Atomic force-EM
Image type
Wide-field
Report size (nm)
60-240
|
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1 - 4 of 4 |
EV-TRACK ID | EV190077 | |||
---|---|---|---|---|
species | Bos taurus | Ascaris suum | Ascaris suum | Ascaris suum |
sample type | Milk | Ascaris suum supernatant | Ascaris suum supernatant | Ascaris suum supernatant |
condition | Control condition | Mycoplasma-contaminated | Control condition | Mycoplasma-contaminated |
separation protocol | DG (d)(U)C milk acidification casein removal SEC | (d)(U)C UF | (d)(U)C qEV UF | (d)(U)C qEV UF |
Exp. nr. | 1 | 3 | 2 | 4 |
EV-METRIC % | 75 | 29 | 17 | 17 |