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You searched for: EV190041 (EV-TRACK ID)
Showing 1 - 9 of 9
Showing 1 - 9 of 9
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV190041 | 1/9 | Homo sapiens | Blood plasma |
(d)(U)C ExoSpin |
Sundar IK | 2019 | 25% | |
Study summaryFull title
All authors
Sundar IK, Li D, Rahman I
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) play a vital role in normal lung physiology to maintain homeostasis in (show more...)
EV-METRIC
25% (56th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Non-smokers
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
ExoSpin Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Pelleting performed
No
Commercial kit
ExoSpin
Other
Name other separation method
ExoSpin
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Rab5b/ TSG101/ Alix/ CD81
Not detected EV-associated proteins
Detected contaminants
Albumin
Not detected contaminants
GRP94/ Calreticulin
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing;Capillary electrophoresis (e.g. Bioanalyzer)
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
|
||||||||
EV190041 | 2/9 | Homo sapiens | Blood plasma |
(d)(U)C ExoSpin |
Sundar IK | 2019 | 25% | |
Study summaryFull title
All authors
Sundar IK, Li D, Rahman I
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) play a vital role in normal lung physiology to maintain homeostasis in (show more...)
EV-METRIC
25% (56th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Smokers
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
ExoSpin Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Pelleting performed
No
Commercial kit
ExoSpin
Other
Name other separation method
ExoSpin
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
Rab5b/ TSG101/ Alix/ CD81
Not detected EV-associated proteins
Detected contaminants
Albumin
Not detected contaminants
GRP94/ Calreticulin
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing;Capillary electrophoresis (e.g. Bioanalyzer)
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
|
||||||||
EV190041 | 3/9 | Homo sapiens | Blood plasma |
(d)(U)C ExoSpin |
Sundar IK | 2019 | 25% | |
Study summaryFull title
All authors
Sundar IK, Li D, Rahman I
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) play a vital role in normal lung physiology to maintain homeostasis in (show more...)
EV-METRIC
25% (56th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
COPD
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
ExoSpin Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Pelleting performed
No
Commercial kit
ExoSpin
Other
Name other separation method
ExoSpin
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
Rab5b/ TSG101/ Alix/ CD81
Not detected EV-associated proteins
Detected contaminants
Albumin
Not detected contaminants
GRP94/ Calreticulin
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing;Capillary electrophoresis (e.g. Bioanalyzer)
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
106.84
EV concentration
Yes
|
||||||||
EV190041 | 7/9 | Homo sapiens | Blood plasma |
(d)(U)C ExoQuick |
Sundar IK | 2019 | 17% | |
Study summaryFull title
All authors
Sundar IK, Li D, Rahman I
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) play a vital role in normal lung physiology to maintain homeostasis in (show more...)
EV-METRIC
17% (46th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Non-smokers
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing;Capillary electrophoresis (e.g. Bioanalyzer)
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV190041 | 8/9 | Homo sapiens | Blood plasma |
(d)(U)C ExoQuick |
Sundar IK | 2019 | 17% | |
Study summaryFull title
All authors
Sundar IK, Li D, Rahman I
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) play a vital role in normal lung physiology to maintain homeostasis in (show more...)
EV-METRIC
17% (46th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Smokers
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing;Capillary electrophoresis (e.g. Bioanalyzer)
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV190041 | 9/9 | Homo sapiens | Blood plasma |
(d)(U)C ExoQuick |
Sundar IK | 2019 | 17% | |
Study summaryFull title
All authors
Sundar IK, Li D, Rahman I
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) play a vital role in normal lung physiology to maintain homeostasis in (show more...)
EV-METRIC
17% (46th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
COPD
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing;Capillary electrophoresis (e.g. Bioanalyzer)
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV190041 | 4/9 | Homo sapiens | Blood plasma |
(d)(U)C Norgen Biotek |
Sundar IK | 2019 | 0% | |
Study summaryFull title
All authors
Sundar IK, Li D, Rahman I
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) play a vital role in normal lung physiology to maintain homeostasis in (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Non-smokers
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Norgen Biotek Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Commercial kit
Norgen Biotek
Other
Name other separation method
Norgen Biotek
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Not detected EV-associated proteins
Detected contaminants
Not detected contaminants
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing;Capillary electrophoresis (e.g. Bioanalyzer)
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
|
||||||||
EV190041 | 5/9 | Homo sapiens | Blood plasma |
(d)(U)C Norgen Biotek |
Sundar IK | 2019 | 0% | |
Study summaryFull title
All authors
Sundar IK, Li D, Rahman I
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) play a vital role in normal lung physiology to maintain homeostasis in (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Smokers
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Norgen Biotek Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Commercial kit
Norgen Biotek
Other
Name other separation method
Norgen Biotek
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Not detected EV-associated proteins
Detected contaminants
Not detected contaminants
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing;Capillary electrophoresis (e.g. Bioanalyzer)
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
|
||||||||
EV190041 | 6/9 | Homo sapiens | Blood plasma |
(d)(U)C Norgen Biotek |
Sundar IK | 2019 | 0% | |
Study summaryFull title
All authors
Sundar IK, Li D, Rahman I
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) play a vital role in normal lung physiology to maintain homeostasis in (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
COPD
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Norgen Biotek Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Commercial kit
Norgen Biotek
Other
Name other separation method
Norgen Biotek
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Not detected EV-associated proteins
Detected contaminants
Not detected contaminants
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing;Capillary electrophoresis (e.g. Bioanalyzer)
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
|
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1 - 9 of 9 |
EV-TRACK ID | EV190041 | ||||||||
---|---|---|---|---|---|---|---|---|---|
species | Homo sapiens | ||||||||
sample type | Blood plasma | ||||||||
condition | Non-smokers | Smokers | COPD | Non-smokers | Smokers | COPD | Non-smokers | Smokers | COPD |
separation protocol | (d)(U)C ExoSpin | (d)(U)C ExoSpin | (d)(U)C ExoSpin | (d)(U)C ExoQuick | (d)(U)C ExoQuick | (d)(U)C ExoQuick | (d)(U)C Norgen Biotek | (d)(U)C Norgen Biotek | (d)(U)C Norgen Biotek |
Exp. nr. | 1 | 2 | 3 | 7 | 8 | 9 | 4 | 5 | 6 |
EV-METRIC % | 25 | 25 | 25 | 17 | 17 | 17 | 0 | 0 | 0 |