Search > Results
You searched for: EV190037 (EV-TRACK ID)
Showing 1 - 1 of 1
Showing 1 - 1 of 1
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV190037 | 1/1 | Homo sapiens | Blood plasma |
Filtration qEV |
Laetitia S. Gaspar | 2020 | 75% | |
Study summaryFull title
All authors
Laetitia S. Gaspar, Magda M. Santana, Carina Henriques, Maria M. Pinto, Teresa M. Ribeiro-Rodrigues, Henrique Girão, Rui Jorge Nobre, Luís Pereira de Almeida
Journal
Methods & Clinical Development
Abstract
Extracellular vesicles (EVs) are membranous structures that protect RNAs from damage when circulatin (show more...)
EV-METRIC
75% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
qEV Protein markers
EV: TSG101/ Analysed only by WB and Dot Blot. We used ELISA to analyse the presence of contaminants/ CD63/ CD81/ GAPDH/ Anxa5/ Alix/ ICAM/ Flotillin1/ LAMP2/ EpCAM
non-EV: Calnexin/ Albumin/ GM130/ ApoB100/ ApoA1 Proteomics
no
Show all info
Study aim
Biomarker/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Filtration steps
> 0.45 µm,
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ LAMP2/ CD81
Not detected EV-associated proteins
GAPDH
Not detected contaminants
Calnexin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Analysed only by WB and Dot Blot. We used ELISA to analyse the presence of contaminants
Detected contaminants
ApoA1/ ApoB100/ Albumin
Other 1
Dot Blot Array
Detected EV-associated proteins
Flotillin1/ CD63/ EpCAM/ ICAM/ Anxa5/ CD81/ TSG101
Not detected EV-associated proteins
Alix
Not detected contaminants
GM130
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;Capillary electrophoresis (e.g. Bioanalyzer)
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
89.04
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
1 - 1 of 1 |
EV-TRACK ID | EV190037 |
---|---|
species | Homo sapiens |
sample type | Blood plasma |
condition | Control condition |
separation protocol | Filtration qEV |
Exp. nr. | 1 |
EV-METRIC % | 75 |