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You searched for: EV190036 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV190036 | 1/3 | Homo sapiens | OX1-19 |
Filtration qEV |
Hicks, David | 2020 | 63% | |
Study summaryFull title
All authors
David A Hicks, Alys C Jones, Nicola J Corbett, Kate Fisher, Stuart M Pickering-Brown, Mark P Ashe, Nigel M Hooper
Journal
Neurochem Res.
Abstract
Healthy brain function is mediated by several complementary signalling pathways, many of which are d (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
qEV Protein markers
EV: TSG101/ CD9
non-EV: Mitofilin/ Grp78 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
OX1-19
EV-harvesting Medium
Serum free medium
Separation Method
Filtration steps
0.22µm or 0.2µm
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ TSG101
Not detected contaminants
Mitofilin/ Grp78
Proteomics database
No
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing
Database
No
Proteinase treatment
No
RNAse treatment
Yes
Moment of RNAse treatment
After
RNAse type
RNase A
RNAse concentration
0.05
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
SEC fraction-dependent
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV190036 | 2/3 | Homo sapiens | OX1-19 |
Filtration qEV |
Hicks, David | 2020 | 63% | |
Study summaryFull title
All authors
David A Hicks, Alys C Jones, Nicola J Corbett, Kate Fisher, Stuart M Pickering-Brown, Mark P Ashe, Nigel M Hooper
Journal
Neurochem Res.
Abstract
Healthy brain function is mediated by several complementary signalling pathways, many of which are d (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
qEV Protein markers
EV: TSG101/ CD9
non-EV: Mitofilin/ Grp78 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
OX1-19
EV-harvesting Medium
Serum free medium
Separation Method
Filtration steps
0.22µm or 0.2µm
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ TSG101
Not detected contaminants
Grp78/ Mitofilin
Proteomics database
No
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing
Database
No
Proteinase treatment
No
RNAse treatment
Yes
Moment of RNAse treatment
After
RNAse type
RNase A
RNAse concentration
0.05
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
SEC fraction-dependent
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV190036 | 3/3 | Homo sapiens | OX1-19 |
Filtration qEV |
Hicks, David | 2020 | 63% | |
Study summaryFull title
All authors
David A Hicks, Alys C Jones, Nicola J Corbett, Kate Fisher, Stuart M Pickering-Brown, Mark P Ashe, Nigel M Hooper
Journal
Neurochem Res.
Abstract
Healthy brain function is mediated by several complementary signalling pathways, many of which are d (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
qEV Protein markers
EV: TSG101/ CD9
non-EV: Mitofilin/ Grp78 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
OX1-19
EV-harvesting Medium
Serum free medium
Separation Method
Filtration steps
0.22µm or 0.2µm
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ TSG101
Not detected contaminants
Mitofilin/ Grp78
Proteomics database
No
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing
Database
No
Proteinase treatment
No
RNAse treatment
Yes
Moment of RNAse treatment
After
RNAse type
RNase A
RNAse concentration
0.05
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
SEC fraction-dependent
EM
EM-type
Transmission-EM
Image type
Wide-field
|
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1 - 3 of 3 |
EV-TRACK ID | EV190036 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Cell culture | ||
cell type | OX1-19 | ||
condition | Control condition | ||
separation protocol | Filtration qEV | Filtration qEV | Filtration qEV |
Exp. nr. | 1 | 2 | 3 |
EV-METRIC % | 63 | 63 | 63 |